breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | 100% | [crl] | [crl] | |
RA | 1,105,093 | T→C | 7.1% | S45P (TCC→CCC) | csgC → | curli assembly protein |
RA | 1,205,173 | A→G | 5.5% | pseudogene (770/1134 nt) | aaaE → | pseudogene, phage terminase protein A family, e14 prophage;Phage or Prophage Related |
RA | 1,286,965 | A→G | 14.4% | intergenic (+439/+101) | narI → / ← rttR | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript |
RA | 1,298,252 | T→C | 6.3% | intergenic (‑131/‑346) | adhE ← / → ychE | fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein |
RA | 1,413,542 | A→T | 100% | Y67N (TAT→AAT) | ydaC ← | DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein |
RA | 1,706,969 | C→T | 6.7% | N17N (AAC→AAT) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
RA | 1,793,897 | Δ1 bp | 100% | coding (213/552 nt) | btuE ← | glutathione peroxidase |
MC JC | 1,978,503 | Δ776 bp | 100% | insB1–insA | insB1, insA | |
RA | 2,093,040 | G→A | 100% | A215T (GCT→ACT) | hisC → | histidinol‑phosphate aminotransferase |
RA | 2,270,600 | T→C | 8.5% | intergenic (+57/‑124) | mepS → / → rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
RA | 2,340,717 | C→T | 5.3% | V1151M (GTG→ATG) | yfaL ← | adhesin |
RA | 2,388,341 | A→G | 5.2% | T28T (ACT→ACC) | yfbN ← | uncharacterized protein |
RA | 2,394,768 | T→C | 13.4% | T93A (ACT→GCT) | nuoL ← | NADH:ubiquinone oxidoreductase, membrane subunit L |
RA | 2,473,882 | T→G | 13.1% | L94R (CTG→CGG) | yfdP → | CPS‑53 (KpLE1) prophage; uncharacterized protein |
RA | 2,501,610 | G→A | 5.6% | L161L (CTG→CTA) | ypdC → | putative DNA‑binding protein |
RA | 2,525,756 | A→G | 6.5% | N211S (AAT→AGT) | yfeN → | putative outer membrane protein |
RA | 2,565,055 | C→G | 5.8% | Q179E (CAG→GAG) | yffS → | CPZ‑55 prophage; uncharacterized protein |
RA | 2,818,143 | T→C | 10.2% | intergenic (‑10/+53) | argZ ← / ← argY | tRNA‑Arg/tRNA‑Arg |
RA | 2,980,742 | A→T | 7.3% | intergenic (‑109/+20) | ygeA ← / ← araE | Asp/Glu_racemase family protein/arabinose transporter |
RA | 3,358,324 | G→A | 5.0% | C1201Y (TGC→TAC) | gltB → | glutamate synthase, large subunit |
RA | 3,436,814 | G→A | 5.8% | R100H (CGC→CAC) | trkA → | NAD‑binding component of TrK potassium transporter |
RA | 3,465,130 | T→C | 6.4% | V17V (GTT→GTC) | gspM → | general secretory pathway component, cryptic |
RA | 3,481,009 | C→A | 7.8% | D51Y (GAT→TAT) | kefG ← | potassium‑efflux system ancillary protein for KefB, glutathione‑regulated |
RA | 4,085,619 | G→T | 7.7% | A68E (GCA→GAA) | fdoG ← | formate dehydrogenase‑O, large subunit |
RA | 4,116,406 | C→T | 7.1% | M272I (ATG→ATA) | glpK ← | glycerol kinase |
RA | 4,117,004 | T→A | 26.2% | D73V (GAT→GTT) | glpK ← | glycerol kinase |
RA | 4,117,050 | T→G | 44.9% | S58R (AGC→CGC) | glpK ← | glycerol kinase |
RA | 4,182,928 | A→T | 12.2% | E562V (GAA→GTA) | rpoB → | RNA polymerase, beta subunit |
RA | 4,187,597 | C→T | 92.0% | P750L (CCG→CTG) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,279,915 | A→G | 6.2% | intergenic (+88/‑64) | ghxP → / → yjcE | guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter |
RA | 4,325,949 | A→G | 6.0% | intergenic (‑207/+451) | yjdN ← / ← yjdM | metalloprotein superfamily protein/zinc‑ribbon family protein |
RA | 4,356,446 | A→T | 7.1% | intergenic (‑34/+25) | dtpC ← / ← cadA | dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible |
RA | 4,356,447 | A→T | 6.4% | intergenic (‑35/+24) | dtpC ← / ← cadA | dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible |
RA | 4,491,466 | G→A | 5.3% | A354V (GCC→GTC) | idnT ← | L‑idonate and D‑gluconate transporter |
RA | 4,544,588 | G→A | 7.2% | G95E (GGA→GAA) | fimC → | periplasmic chaperone |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 224344 | NA (NA) | 3 (0.040) | 3/270 | NT | NA | noncoding (574/1542 nt) | rrsH | 16S ribosomal RNA of rrnH operon |
? | NC_000913 | = 224358 | NA (NA) | noncoding (588/1542 nt) | rrsH | 16S ribosomal RNA of rrnH operon | |||||
* | ? | NC_000913 | 1207790 = | 25 (0.300) | 45 (0.620) | 37/244 | NT | 64.2% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 28 (0.380) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 23 (0.280) | 63 (0.870) | 52/244 | NT | 72.3% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 28 (0.380) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 72 (0.900) | 59/268 | NT | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | = 2729041 | NA (NA) | 6 (0.080) | 6/262 | NT | NA | noncoding (142/2904 nt) | rrlG | 23S ribosomal RNA of rrnG operon |
? | NC_000913 | = 2729045 | NA (NA) | noncoding (138/2904 nt) | rrlG | 23S ribosomal RNA of rrnG operon | |||||
* | ? | NC_000913 | 2729473 = | 53 (0.640) | 4 (0.050) | 3/256 | NT | 7.9% | intergenic (‑31/+141) | gltW/rrsG | tRNA‑Glu/16S ribosomal RNA of rrnG operon |
? | NC_000913 | = 4168362 | 44 (0.580) | intergenic (+163/‑9) | rrsB/gltT | 16S ribosomal RNA of rrnB operon/tRNA‑Glu | |||||
* | ? | NC_000913 | 2729953 = | NA (NA) | 3 (0.040) | 4/264 | NT | NA | noncoding (1203/1542 nt) | rrsG | 16S ribosomal RNA of rrnG operon |
? | NC_000913 | 2729970 = | NA (NA) | noncoding (1186/1542 nt) | rrsG | 16S ribosomal RNA of rrnG operon |