Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,340,717	C→T	5.3%	V1151M (GTG→ATG)	yfaL ←	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,340,717	0	C	T	5.3%	252.8 / 3.1	87	V1151M (GTG→ATG)	yfaL	adhesin
Reads supporting (aligned to +/- strand):  ref base C (31/51);  new base T (2/3);  total (33/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAGTGGTAATGAT  >  NC_000913/2340571‑2340863                                                                                                                                                   |                                                                                                                                                   gATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGGATATCATCACCCTGCGCTTGTGCCAcgc                                                                                                                                                  >  1:606531/1‑149 (MQ=255) gATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACAcgc                                                                                                                                                  >  2:51969/1‑149 (MQ=255)  aTAGTGCTGCCATCCTCTTCAATTTCCGTAGAATGGGAATCGTGATAATAATTCAGATCTAATATTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACAcgcg                                                                                                                                                 >  1:947019/1‑149 (MQ=255)  aTAGTGCTGCAATCCTCTTCAATTTCCGTCGACTGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACAcgcg                                                                                                                                                 <  1:748982/149‑1 (MQ=255)  aTAGCGCTGCCATCCTCTTCAATTTCCGGCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGAACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACAcgcg                                                                                                                                                 <  1:973382/149‑1 (MQ=255)     gTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACACCGGCTGGTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGCCACgcgcgc                                                                                                                                              >  1:1427803/1‑149 (MQ=255)       gctgcCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCAc                                                                                                                                            >  1:280629/1‑149 (MQ=255)       gctgcCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCAc                                                                                                                                            <  1:502542/149‑1 (MQ=255)         tgcCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACCGCGGTACGCCATTCGCTGTGTAAACCCAGACGCGTCTGTATCTCATCAACCTGCGATTGTGACACGCGAGCGCgg                                                                                                                                          >  2:41677/1‑149 (MQ=255)         tgcCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACgg                                                                                                                                          <  2:443759/149‑1 (MQ=255)          gcCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCAGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGt                                                                                                                                         <  2:602043/149‑1 (MQ=255)              tCCGCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAgc                                                                                                                                     <  2:463517/149‑1 (MQ=255)               ccTCTTCAATTTCCGTCGAATGGGGAGCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAgcg                                                                                                                                    <  2:484898/149‑1 (MQ=255)                ctctTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAgcgg                                                                                                                                   <  2:171449/149‑1 (MQ=255)                       aTTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTTGTATGACATGAGCAACGGTACACCATTCGCTGTGTAAACCCAGCCGCGTCTGAATACGCTCCCCCTGCGCTTCTGACACGCGCGCACGGTTAGCGGCGGTaac                                                                                                                            >  2:311269/1‑148 (MQ=255)                       aTTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTaaa                                                                                                                            <  1:87625/149‑1 (MQ=255)                        tttCCGTCGAATGGGGATCGTGATAATAATTCAGAGCTCATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTaaaa                                                                                                                           <  2:1098798/149‑1 (MQ=255)                         ttCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAat                                                                                                                          <  1:594485/149‑1 (MQ=255)                         ttCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACACGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAat                                                                                                                          <  2:436242/149‑1 (MQ=255)                           ccGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatca                                                                                                                        <  1:150737/149‑1 (MQ=255)                            cGTCGAATGGGGATCGTGATAATAATTCAGATCTACTGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCCCGGTTAGCGGCGGGaaaaccct                                                                                                                       >  2:421604/1‑145 (MQ=255)                             gacGAAGGGGGATCGTGATGATAATGCAGATCTGATGTTGGTGTGGCATGAACAGCGGTACGCCACCCGCTGTGTCCACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcatc                                                                                                                      <  1:139112/147‑1 (MQ=255)                             gTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcatc                                                                                                                      <  1:927853/149‑1 (MQ=255)                             gTCGAATGGGGATCGTGATAATAATTCAGATCTAATGCTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTCCGATCGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcatc                                                                                                                      <  1:927834/149‑1 (MQ=255)                                gAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCCCGGTTAGCGGCGGTAAAATCATCctg                                                                                                                   >  2:1063154/1‑149 (MQ=255)                                   tGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGCTTGTGACACGCCCGCACGGTTAGCGGCGGTAAAATCATCctgctg                                                                                                                >  2:105459/1‑149 (MQ=255)                                           gTGATAATAATTCAGATCTAATGTTGGTATGACAGGAACAGCGGTACGCCATTCGCTGGGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCt                                                                                                        <  2:128455/149‑1 (MQ=255)                                              ataataATTCAGATCTAATGTGGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGAt                                                                                                     <  2:457915/149‑1 (MQ=255)                                                aataatTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTTCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCCCGCCCTGATaa                                                                                                   >  1:700874/1‑149 (MQ=255)                                                aataatTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATaa                                                                                                   >  1:700943/1‑149 (MQ=255)                                                  taatTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACATGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAAt                                                                                                 <  2:272454/149‑1 (MQ=255)                                                  taatTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACATGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAAt                                                                                                 >  1:597219/1‑149 (MQ=255)                                                  taatTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACATGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAAt                                                                                                 >  1:176378/1‑149 (MQ=255)                                                    atTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACGCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCa                                                                                               >  1:415359/1‑149 (MQ=255)                                                     ttCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCGCGGTTAGCGGCGGTAAAATCATCCTGCTGCCGCCCCTGATAAATCAc                                                                                              >  2:589794/1‑149 (MQ=255)                                                     ttCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGCTAAATCAc                                                                                              >  2:225979/1‑149 (MQ=255)                                                       cAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCAcct                                                                                            <  2:268506/149‑1 (MQ=255)                                                        aGATCTCATGTTGGTATCACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCAcctg                                                                                           <  2:948093/149‑1 (MQ=255)                                                        aGATCTACTGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGAGAAATCAcctg                                                                                           <  1:197622/149‑1 (MQ=255)                                                         gATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCAcctgc                                                                                          >  1:425858/1‑149 (MQ=255)                                                         gATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCAcctgc                                                                                          <  2:725593/149‑1 (MQ=255)                                                          aTCTAATGTTGGTATGACATGAACAGCGGTACGCCATTAGCAGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGGAAAATCATCCTGCTGCACGCCCTGATACATCCactgcg                                                                                         >  1:421558/1‑149 (MQ=255)                                                            cTAATGTTGGTACGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCAcctgcgcc                                                                                       <  2:919918/149‑1 (MQ=255)                                                                tGTTGGTATGACATGAACAGCGGTACGCCATTTGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCTGGGTTAGCGGGGGTAAAATCTTTCTGCGGCACGTGCTGTTAAATCGcctgggcctgcg                                                                                   >  2:235311/1‑149 (MQ=255)                                                                tGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTAAGCAGCGGTAAAATCATCATGCTACACGCCCTGATAAATCAcctgcgcccccg                                                                                   >  2:235375/1‑145 (MQ=255)                                                                ggTTGGCATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCAcctgcgcctgcg                                                                                   <  1:1305035/148‑1 (MQ=255)                                                                 gTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCgg                                                                                  >  2:152767/1‑149 (MQ=255)                                                                    ggTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCCGATAAATCACCTGCGCCTGCGGTTc                                                                               >  2:1190229/1‑149 (MQ=255)                                                                         gACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCATCCGCGTCTGAATATCATCACCCTGTGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATca                                                                          <  2:272159/149‑1 (MQ=255)                                                                         gACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATca                                                                          <  2:842708/149‑1 (MQ=255)                                                                          acaaaaaCAGCGGCACGCCATTCGCTGGGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATcac                                                                         <  2:358336/144‑1 (MQ=255)                                                                          aCATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATcac                                                                         <  1:1127874/149‑1 (MQ=255)                                                                              gAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATcaccaca                                                                     <  1:883225/149‑1 (MQ=255)                                                                                 cAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATcaccacacca                                                                  <  2:871699/149‑1 (MQ=255)                                                                                      gTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGccc                                                             <  2:984164/149‑1 (MQ=255)                                                                                          gCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGt                                                         <  2:1427803/149‑1 (MQ=255)                                                                                            cATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACATGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTaa                                                       <  2:176378/149‑1 (MQ=255)                                                                                            cATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACATGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTaa                                                       <  2:597219/149‑1 (MQ=255)                                                                                            cATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCAACACTCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTaa                                                       <  1:35293/149‑1 (MQ=255)                                                                                             aTTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAAc                                                      <  1:460216/149‑1 (MQ=255)                                                                                             aTTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGAGGCGGTAAAATCATCCTGCTGCACGCCCTGGAAAATCACCGGCGCCTGCGGGTCAATCACCACGCCACACCCCGGtcaa                                                      >  2:1129259/1‑146 (MQ=255)                                                                                              ttCTCTGGGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACCCCCCGGTaaac                                                     >  2:617045/1‑147 (MQ=255)                                                                                              ttCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAAcc                                                     >  1:742799/1‑149 (MQ=255)                                                                                              ttCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAAcc                                                     >  1:593833/1‑149 (MQ=255)                                                                                               tCGCCGTGCAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGTCACTCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCa                                                    <  1:1299401/149‑1 (MQ=255)                                                                                                  cTGTGTAAACCCAGCCGCGTCTGAATATCATAACCCTGCGCATGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCGGCGGTTCAATCACCAAACCACGCCCCGGCAAcccagg                                                 >  2:1205471/1‑145 (MQ=255)                                                                                                   tgtgTATACCCAGCCGCGTCTGAATATCATCACCCTCCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGa                                                <  2:427653/149‑1 (MQ=255)                                                                                                   tgtgTAACCCCAGCCGCGGCTGAATATCACCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGa                                                <  1:191894/149‑1 (MQ=255)                                                                                                      gtAGACCCGGCCGCGTCTGAAGTTCATCACCGTGCGATTGTGGCACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGTTATATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATAc                                             <  2:373131/149‑1 (MQ=255)                                                                                                         aaCCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAACCACCCCACCAACCCCCGGTCACCACTGCTAccac                                          >  1:552219/1‑147 (MQ=255)                                                                                                          aCCCCGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCg                                         <  2:1108163/149‑1 (MQ=255)                                                                                                               gCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTcc                                    <  1:433606/149‑1 (MQ=255)                                                                                                                    gTCTGAATATCATCCCCCTGCGATTGTGACACCCGCGCACGTTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGa                               <  1:1355117/149‑1 (MQ=255)                                                                                                                    gTCTGAATATCATCACCCTGCGATGGTGACCCGCGCGCACTGCTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGa                               <  1:1355121/149‑1 (MQ=255)                                                                                                                     gccgAATAACACCCCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAg                              <  1:163985/146‑1 (MQ=255)                                                                                                                      cTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAgg                             <  1:225979/149‑1 (MQ=255)                                                                                                                        gAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCg                           >  1:1189726/1‑149 (MQ=255)                                                                                                                         aaTATCATCACCCTGCGATTGTGACACGCGCGCCCGGTCAGCGGCGTTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTCACCACTGATACCCCGCCTCCAGCGAGGCGa                          <  1:761572/149‑1 (MQ=255)                                                                                                                         aaTATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGa                          <  2:280629/149‑1 (MQ=255)                                                                                                                         aaTATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGa                          <  1:51969/149‑1 (MQ=255)                                                                                                                         aaTATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGa                          <  2:556029/149‑1 (MQ=255)                                                                                                                             tcatcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCCTCATGATGAATCGCCGGATACATAAAAAATTTCCTCGGTTCAATCACCTCACCACTCCGCGTTAACCACTGATACCCCGCCTCCAGCGAGGCGATAAt                      >  1:1433833/1‑149 (MQ=255)                                                                                                                              catcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCTATAATc                     >  1:779141/1‑149 (MQ=255)                                                                                                                              catcaCCCTGCGAGTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACCCCCCGCCCGGGTAACCACTGATACCCCGCCTCCAGCGCGGCGATAATc                     >  2:1390959/1‑149 (MQ=255)                                                                                                                                    ccTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCcgac               <  2:700874/149‑1 (MQ=255)                                                                                                                                    ccTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCcgac               <  2:700943/149‑1 (MQ=255)                                                                                                                                      tGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCcgccga             >  1:1080459/1‑149 (MQ=255)                                                                                                                                      tGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCcgacga             <  2:425858/149‑1 (MQ=255)                                                                                                                                       gCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAg            >  2:583895/1‑149 (MQ=255)                                                                                                                                           ttGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTTCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAGTGGt        >  2:131723/1‑149 (MQ=255)                                                                                                                                           ttGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAGTGGt        <  1:1361119/149‑1 (MQ=255)                                                                                                                                            tgtgACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAGTGGTa       <  1:152767/149‑1 (MQ=255)                                                                                                                                                 cccgcgcgCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACTCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAGTGGTAATGAt  <  1:978283/147‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCAGATCTAATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCACCTGCGCCTGCGGTTCAATCACCACACCACGCCCCGGTAACCACTGATACCCCGCCTCCAGCGAGGCGATAATCCCCGACGAGTGGTAATGAT  >  NC_000913/2340571‑2340863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑