Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,0400GA100.0% 224.0 / ‑6.7 73A215T (GCT→ACT) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (36/34);  minor base C (0/1);  total (36/37)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092900‑2093185
                                                                                                                                            |                                                                                                                                                 
ttCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGct                                                                                                                                           <  2:1054471/149‑1 (MQ=255)
   gCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGCGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTg                                                                                                                                        >  1:419889/1‑149 (MQ=255)
       ccTGCCGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCCCTGGCgg                                                                                                                                    >  1:404562/1‑149 (MQ=255)
         tgctgGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCgggg                                                                                                                                  >  1:198476/1‑149 (MQ=255)
              gcgTTGACCCGCGGTACGGCGATTGTGGTTGCCGATGAAGCCTATCTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCg                                                                                                                             <  2:807644/147‑1 (MQ=255)
                gTTAACCCGCGGTAAGGCGCTTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGTTGGCTGGCTGGCGGAATATAGGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                           >  1:1058253/1‑149 (MQ=255)
                gTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGtt                                                                                                                           <  2:437834/149‑1 (MQ=255)
                  tAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTCTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAATCTTTTGCTCTGGCGGGGCTTCGTTGc                                                                                                                         <  2:530195/149‑1 (MQ=255)
                    aCCGGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                       <  2:180061/149‑1 (MQ=255)
                     cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCCTCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACACACACTGTCGAAAACTTTTGCCCTGGCGGGGCTGCGTTGCggg                                                                                                                      >  2:1357273/1‑148 (MQ=255)
                     cccGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATACCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGa                                                                                                                      <  2:807623/149‑1 (MQ=255)
                       cgcgGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCTGCAGGCATCGCTAGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGAtt                                                                                                                    <  2:999378/149‑1 (MQ=255)
                              aGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTg                                                                                                             >  1:505226/1‑149 (MQ=255)
                               ggtggttGTGGTTGCCGACGAATCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCCCCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTgg                                                                                                            <  2:792673/144‑1 (MQ=255)
                                  gATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTATGGCGGGGCTTCGTTGCGGATTTACGCTGGCaa                                                                                                         <  1:934646/149‑1 (MQ=255)
                                        ggTTGCCGATGAAGCCTATCGCGAGTTTTGCCCGCAGGCATCGT‑‑‑TGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGt                                                                                               <  2:600873/149‑1 (MQ=255)
                                          ttGCCGATGTCGCCTATATCGAGTGTTGCCCGCAGGCATCGCTGGCTGGTTGGCTGGCGGAATATCCGCACCTGGCTAGTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 <  2:692278/149‑1 (MQ=255)
                                          ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGGTGGCTGGCGGAAGATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagca                                                                                                 >  1:784972/1‑147 (MQ=255)
                                          ttGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaagaa                                                                                                 >  1:1037264/1‑149 (MQ=255)
                                           tGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGaagaag                                                                                                <  1:128102/149‑1 (MQ=255)
                                              cGATGAAGCCTATATCGAGTTTTACCCGCAGGCATCGCTGGCTGGCTGGCTGGAGGAATATCGGGACCTGGCTATTTTACGCACACTGTCGGAAACTTTTGCTCTGGCGGGGCTCCGTTGCGGTTTGACCCTGGCAAAGGAAGAAGtca                                                                                             >  1:47860/1‑149 (MQ=255)
                                                aTGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGtcatc                                                                                           >  2:1233809/1‑149 (MQ=255)
                                                  gAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCaa                                                                                         <  1:1000376/149‑1 (MQ=255)
                                                   aaGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGTAGAAGTCATCAAc                                                                                        <  1:994636/149‑1 (MQ=255)
                                                       cTATCTCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                    <  2:896811/149‑1 (MQ=255)
                                                       cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCCAACGAAGAAGTCATCAACctgc                                                                                    >  2:591109/1‑149 (MQ=255)
                                                       cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                    <  2:1290145/149‑1 (MQ=255)
                                                       cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACctgc                                                                                    <  2:38072/149‑1 (MQ=255)
                                                       cTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGCGGCATCGTTGCGGGTTTACGCTGCCGAACGCAGAGGTCACCAcccccc                                                                                    >  2:77610/1‑146 (MQ=255)
                                                            tCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGCAGATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGGGGAGTTACGCTGGCAACCGAAGCAGTGATCACCCTGCtgagg                                                                               >  2:1357084/1‑147 (MQ=255)
                                                              gAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGaa                                                                             >  1:1180631/1‑149 (MQ=255)
                                                                gTTTGGCCCGGGGGAATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCGTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAg                                                                           <  1:186319/149‑1 (MQ=255)
                                                                 ttttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGt                                                                          >  2:1248197/1‑149 (MQ=255)
                                                                   ttGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGa                                                                        <  1:1274628/149‑1 (MQ=255)
                                                                    tGCCCTCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGAt                                                                       <  2:335403/149‑1 (MQ=255)
                                                                     gCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATc                                                                      <  2:247193/149‑1 (MQ=255)
                                                                      cccGCAGGCCTCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCg                                                                     <  1:188967/149‑1 (MQ=255)
                                                                      cccGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCCAACGAAGACGTCATCAACCCGCCGATGAAAGCGATCg                                                                     >  2:200595/1‑149 (MQ=255)
                                                                           aGGCATCGCCGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTGTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGccccc                                                                <  1:484042/149‑1 (MQ=255)
                                                                             gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGTGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGGCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                              >  2:1360171/1‑149 (MQ=255)
                                                                             gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCtc                                                              >  2:720445/1‑148 (MQ=255)
                                                                             gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTa                                                              <  2:758092/149‑1 (MQ=255)
                                                                             gCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCCTCGTTGCGGATTTACGCCGGCAAACGAAGGAGTCATCAACCCGCTGACGCAAGTGCACAcccccca                                                              >  2:503488/1‑147 (MQ=255)
                                                                               ctCGCTGGCTGGCTGGCTGGCGGAATATCCTCCCCTGGCTATTTTCCGCACCCTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTGATCAACCTGCTGATGAAAGTGATCGCCCCCTAcc                                                            <  1:735330/148‑1 (MQ=255)
                                                                               aTCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAcc                                                            >  1:524531/1‑149 (MQ=255)
                                                                                tCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAccc                                                           >  1:1148375/1‑149 (MQ=255)
                                                                                tCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTAccc                                                           <  1:1233809/149‑1 (MQ=255)
                                                                                   ctggctggctggctggCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGct                                                        >  1:1214821/1‑149 (MQ=255)
                                                                                       ctggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGCTACGCGGCTGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCg                                                    >  1:699153/1‑149 (MQ=255)
                                                                                       ctggctggctggcGGAATATCCGCACCGGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCg                                                    <  2:71973/149‑1 (MQ=255)
                                                                                        tggctggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGACAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGa                                                   <  2:167240/149‑1 (MQ=255)
                                                                                            tggctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGcc                                               >  1:373385/1‑149 (MQ=255)
                                                                                             gggtggcGGCATGCCCGCACCTGGCTATTTTTCACACACTGTCGAAAACTTTTTCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCg                                              <  2:426380/146‑1 (MQ=255)
                                                                                              gctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCCCTCGACGCCgg                                             >  1:667508/1‑149 (MQ=255)
                                                                                              gctggcGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCGCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCgg                                             <  2:765594/149‑1 (MQ=255)
                                                                                              gctggcGGAATATCCGCACCTGGCTAATTTACGCACACGGTCGAAAACTTTTGCCCTGACGGGGCTTAGATGCGGAATAACGCTGGCAAACGAAGAAGACATCAACCTACCGATGAAAGGGATCGCCCACTACCACATCTCGACGCCga                                             >  2:470779/1‑148 (MQ=255)
                                                                                                           ccGCACCTGGCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACACTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGc                                >  2:818940/1‑149 (MQ=255)
                                                                                                                 cTGGCTATTTTACGCACACTGTCGAAAACGTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCa                          >  1:1025936/1‑149 (MQ=255)
                                                                                                                   ggCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAgg                        <  1:255250/149‑1 (MQ=255)
                                                                                                                    gCTATTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGc                       >  1:229937/1‑149 (MQ=255)
                                                                                                                      tgttttACGCACACTGTCGAAACCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGt                     <  2:1109611/147‑1 (MQ=255)
                                                                                                                       aTTTTACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGtt                    <  1:1193131/149‑1 (MQ=255)
                                                                                                                          ttgcGCACACGGTCGGAAGCTGTTGCTCTGGGGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGGGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAg                 <  2:268174/146‑1 (MQ=255)
                                                                                                                          ttACTCTCACTGGCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAg                 <  1:262928/149‑1 (MQ=255)
                                                                                                                          ttACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGCAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAg                 >  2:1264745/1‑149 (MQ=255)
                                                                                                                           tACGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGCGTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGc                <  1:112230/149‑1 (MQ=255)
                                                                                                                             cGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCCAACGAAGACGTCACCAACCTGCTGATGAAAGTGACCGCCCCCAACCCGCTCTCGCCGCCGGTTGCCGACATTGCGGCCCAGGCGCTAAGccc              >  2:73850/1‑149 (MQ=255)
                                                                                                                             cGCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCACTAACCGCTCTCGACGCCGGTTGCCGACATTGCGGCCGAGGCGTTTGGccc              >  2:73359/1‑149 (MQ=255)
                                                                                                                              gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCTCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAGGcccg             >  1:211555/1‑148 (MQ=255)
                                                                                                                              gCACACTGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCca             >  1:971251/1‑149 (MQ=255)
                                                                                                                                    tGTCGAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGa       <  2:198476/149‑1 (MQ=255)
                                                                                                                                        gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGGAtcg   >  1:177615/1‑149 (MQ=255)
                                                                                                                                        gAAAACTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATCGCGGCCCAGGCGTTAAGCCCACGCGGAAtcg   >  1:219291/1‑149 (MQ=255)
                                                                                                                                         aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  2:723370/1‑149 (MQ=255)
                                                                                                                                         aaaaCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAAtcgt  >  2:862159/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGT  >  NC_000913/2092900‑2093185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: