Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,270,600 T→C 8.5% intergenic (+57/‑124) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,270,6000TC 8.5% 186.4 / 6.2 68intergenic (+57/‑124)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (36/25);  new base C (4/2);  total (41/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

CCACCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTT  >  NC_000913/2270452‑2270747
                                                                                                                                                    |                                                                                                                                                   
ccaccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCt                                                                                                                                                     <  2:1181752/149‑1 (MQ=255)
         gtggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg                                                                                                                                            <  1:655476/149‑1 (MQ=255)
         gtggtgTTATTATTTCCAGCATGAATGAACCGGACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg                                                                                                                                            <  2:198801/149‑1 (MQ=255)
          tggtgTCATTATATCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGTGTTCTCAGCCGCAGCTAATAACCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGc                                                                                                                                           <  1:492472/149‑1 (MQ=255)
           ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCt                                                                                                                                          >  2:500207/1‑149 (MQ=255)
                 attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTg                                                                                                                                    >  2:129829/1‑149 (MQ=255)
                 attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGCtttcagt                                                                                                                                    <  2:819394/149‑5 (MQ=255)
                   tattTCCACCTTGAATGAACCGTCCTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGtt                                                                                                                                  <  1:927722/149‑1 (MQ=255)
                      ttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttc                                                                                                                               >  1:608297/1‑149 (MQ=255)
                         cAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCCCTGCTTAGGCATTGCTTTTTTGTTttcatt                                                                                                                            >  2:80975/1‑149 (MQ=255)
                         cAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcatt                                                                                                                            >  1:1452458/1‑149 (MQ=255)
                          aGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcattc                                                                                                                           >  1:266897/1‑149 (MQ=255)
                           gCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcattca                                                                                                                          >  1:785925/1‑149 (MQ=255)
                            cATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTAAATTGCATCGAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTttcattcag                                                                                                                         <  1:1425717/149‑2 (MQ=255)
                                   gaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGGGCTTTTTTGTTTTCATTCATCGGAGaa                                                                                                                  >  2:1147485/1‑149 (MQ=255)
                                     aCCGTACCTGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGaaaa                                                                                                                <  1:394644/149‑1 (MQ=255)
                                            tGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGtt                                                                                                         >  1:622580/1‑149 (MQ=255)
                                             ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGttt                                                                                                        <  2:240606/149‑1 (MQ=255)
                                              gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTGATCAGAGAAAATGATGTTTc                                                                                                       >  1:752518/1‑149 (MQ=255)
                                                agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg                                                                                                     >  1:104662/1‑149 (MQ=255)
                                                 gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgc                                                                                                    <  1:1173426/149‑1 (MQ=255)
                                                    gCGTTACAAGGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACCGAAAGCACTGCTTAGGCAGTGCTTTTCTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTc                                                                                                 <  1:48171/149‑1 (MQ=255)
                                                    gCGTTACAACGCAGCACGCCGTGTTCTCAGCCGCAGCTAATATACCGTTTTCATGCAATCCCCTGGCTTCCCTGACGAGTGAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTc                                                                                                 <  2:522777/149‑1 (MQ=255)
                                                    gCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTc                                                                                                 >  2:927604/1‑149 (MQ=255)
                                                     cGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCt                                                                                                >  2:653481/1‑149 (MQ=255)
                                                        tACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGa                                                                                             <  2:1159331/149‑1 (MQ=255)
                                                        tACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCAATGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGAGGTTTCCGCGTCTTGa                                                                                             >  2:963079/1‑149 (MQ=255)
                                                         aCAACCAAGCACGCCCGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGAt                                                                                            <  2:825255/149‑1 (MQ=255)
                                                           aaCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCGGAGAAAATGTTTTCCGCGTCTTGATCCAg                                                                                        >  2:478628/1‑149 (MQ=255)
                                                           aaCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGTTTTCCGCGTATTGATCCAg                                                                                        >  2:478581/1‑149 (MQ=255)
                                                           aaCGAAGCACGCCGGGTTCTAAGCAGCAGATAATAAACCGTTAGGAAGCAATCCCTTGGCTATCCCTAAGAGCTAACTGAAAGCTCCGCGTACGCCGTGATTTCTTGCTTTTATTCATCAGAGAAAATGATGTTTCCGCTTCTTGCTcc                                                                                          >  2:715356/1‑149 (MQ=255)
                                                            aCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCa                                                                                         <  1:1147059/149‑1 (MQ=255)
                                                             cGAAGCACGCCGGGTTCCCAGCCGCAGCTAACAACCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGGTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAACATGATGTTTCCGCGTCTTGATCCAg                                                                                        <  1:977877/149‑1 (MQ=255)
                                                               aaGCACGCCGGGATCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAgcc                                                                                      >  2:1258211/1‑147 (MQ=255)
                                                                     gCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCTCCTTGATCCAGTCtataat                                                                                >  2:416182/1‑147 (MQ=255)
                                                                     gCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGt                                                                                <  2:1373369/149‑1 (MQ=255)
                                                                       cGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAGTCCCTTGGCTATCCTGACGAGTACACTGAGAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTcg                                                                              >  2:1319992/1‑148 (MQ=255)
                                                                        gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCg                                                                             <  1:1203627/149‑1 (MQ=255)
                                                                              tccgCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAACGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAtt                                                                       <  1:1173643/146‑1 (MQ=255)
                                                                              tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAtt                                                                       <  2:252500/149‑1 (MQ=255)
                                                                              tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGCGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAtt                                                                       >  1:1150269/1‑149 (MQ=255)
                                                                                     cAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGGGAAAATGGTGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt                                                                >  1:36250/1‑149 (MQ=255)
                                                                                              aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGGTGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGGCATTGTTATCTTTTACAtct                                                       >  2:51639/1‑147 (MQ=255)
                                                                                              aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt                                                       >  2:658599/1‑149 (MQ=255)
                                                                                              aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATACAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt                                                       >  1:702066/1‑149 (MQ=255)
                                                                                                ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTAGAGTCCGGGCCTTGTTATCTTTTAAAggttg                                                     >  2:962170/1‑149 (MQ=255)
                                                                                                      ggATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTaa                                               >  1:254293/1‑149 (MQ=255)
                                                                                                       gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTTTTTTTTTTTTTCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAt                                              >  2:1427391/1‑149 (MQ=255)
                                                                                                           taaTCCCTTGGCTATCCTGACGAGTTAACTGAATGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTTCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa                                          <  1:1034255/148‑1 (MQ=255)
                                                                                                             aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg                                        >  1:467491/1‑149 (MQ=255)
                                                                                                             aTCCCTTGGCTATCCTGACGAGTTAACTGAAACCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTACCTTTTAAACGTCGTCGTAATCTCAga                                        >  2:1034255/1‑148 (MQ=255)
                                                                                                                ccTTGGCTATCCTGACGGGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGaaa                                     >  2:856445/1‑149 (MQ=255)
                                                                                                                ccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATCTTGTCGTAATTTCAGGaga                                     >  1:701375/1‑147 (MQ=255)
                                                                                                                  ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTTTTTTTTTTTTTAAGAGAAAAGGGTATTTCCGCGTCTTGATCCAGGCGAGAGTCCGGTCCTTGTTATCGTTTAAATGTGTTCGTAGTTGAAGGAAAta                                   >  2:1354972/1‑148 (MQ=255)
                                                                                                                       tATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTCATCTTTTAAATGTTGTCGTGATTTCAGGAAATTAACgg                              >  1:832754/1‑149 (MQ=255)
                                                                                                                                gAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTAACTTGTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                     <  2:456675/149‑1 (MQ=255)
                                                                                                                                 aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATTGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc                    <  2:893223/149‑1 (MQ=255)
                                                                                                                                 aGTTAACTGAAAGCACTGCTTAGGCAGTGCTAATATCTTGCCACTGATCATACAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTAGTAATTTCAGGAGATTAACGGAATCATGTTc                    <  2:305083/149‑1 (MQ=255)
                                                                                                                                 aGTTAACTGAAAGCACTGCCTAGGCAGTGCTTTTTTGTGTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc                    <  1:552396/149‑1 (MQ=255)
                                                                                                                                  gTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                   <  1:895828/149‑1 (MQ=255)
                                                                                                                                   aaacaaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt                  >  1:1312229/8‑149 (MQ=255)
                                                                                                                                   aaacaaaaaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt                  >  2:388711/8‑149 (MQ=255)
                                                                                                                                      caaaaaaGCACTGCCTAAGCAGTGCTTGTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGACCCAGGCTCTAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcg               >  1:781165/5‑149 (MQ=255)
                                                                                                                                       cTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc              >  1:247206/1‑149 (MQ=255)
                                                                                                                                        tGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgcg             >  2:1315133/1‑149 (MQ=255)
                                                                                                                                        tGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgcg             >  1:105829/1‑149 (MQ=255)
                                                                                                                                          aaaGCACTGCCTAAGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCt           >  2:765465/1‑149 (MQ=255)
                                                                                                                                           aaGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTc          <  2:108571/149‑1 (MQ=255)
                                                                                                                                                   cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt  <  2:105829/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCACCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTT  >  NC_000913/2270452‑2270747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: