Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,742 A→T 7.3% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7420AT 7.3% 290.4 / 12.5 109intergenic (‑109/+20)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (56/40);  new base T (6/2);  total (64/45)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

Only 100 of 109 total aligned reads displayed.

TTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGT  >  NC_000913/2980595‑2980889
                                                                                                                                                    |                                                                                                                                                   
ttCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATGTCTATTAACGAAAAAAGGGCCGGATGTACAg                                                                                                                                                    >  1:979534/1‑149 (MQ=255)
   cctgCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCACCGGCCAAGTGCCCAATCTCTAGTAACGAAAAAAGGGCCGGATGAACAGcac                                                                                                                                                 >  2:926593/4‑149 (MQ=255)
   ccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATCAGGAACGAGACAAACGCCTCAACGGCCAGGTGCCCAATCTCTATTACCGAAAAAAGGGCCGGATGTCCGGcac                                                                                                                                                 >  2:480747/1‑149 (MQ=255)
   ccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAAGAAGGAACGGGACAAACGCCTCACCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGgac                                                                                                                                                 >  1:191499/1‑149 (MQ=255)
   ccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCCGATGTGTGTTACCGCAACAAGGATGCATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCGCTATTAACGAAAAAAGGGCCGGATGTACAGcac                                                                                                                                                 >  2:1212565/1‑149 (MQ=255)
    cAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGACCGGGTCTAACAGCCTAATGGCCAAGCTGCCAGTCGCTATTAACGAAAAAATGGCCGGATGTACAGcaca                                                                                                                                                >  2:1027984/1‑149 (MQ=255)
    cAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                                                                                >  2:1190221/1‑149 (MQ=255)
       cTCATTCCTCCCAGCAACCCCATGGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATcc                                                                                                                                             <  1:319970/149‑1 (MQ=255)
         cATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCgg                                                                                                                                           <  1:1169822/149‑1 (MQ=255)
         cATTCCCCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCgg                                                                                                                                           <  1:435708/149‑1 (MQ=255)
           ttCCTCCCAGCAAACCAATTGTTTTCATTCCTTTCTCCTGTTGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGGCCAGTCTTTATTAACGAAAAAAGGGGCGGATGTACTGCACATCTGGcc                                                                                                                                         >  2:64089/1‑149 (MQ=255)
           ttCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGACGTAACGCCCATCCGGcc                                                                                                                                         >  2:292488/1‑149 (MQ=255)
                 ccAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaa                                                                                                                                   >  2:760951/1‑149 (MQ=255)
                  cAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGACTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCCCATCCGGCCCGTGaaa                                                                                                                                  >  1:1196170/1‑149 (MQ=255)
                   aGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACGTGGCTGAATAGGGAACGAGACCAACGCCTCAACGGCGGAGTGCCCAGTCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                 <  1:784152/149‑1 (MQ=255)
                   aGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                 >  1:968148/1‑149 (MQ=255)
                   aGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                 <  1:380909/149‑1 (MQ=255)
                         ccAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACg                                                                                                                           >  1:375778/1‑149 (MQ=255)
                           aaTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAGGGAACGAGACAAACGCATCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGACATCAGACGcc                                                                                                                         >  1:369584/1‑149 (MQ=255)
                            aTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGAGGTACAGCACATCCGGCCCGGGAAATCAGACGCCg                                                                                                                        >  2:696319/1‑149 (MQ=255)
                                  ttCACTCCTCTGTCCTGATGTTTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAttt                                                                                                                  <  1:1081975/149‑1 (MQ=255)
                                   tCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtc                                                                                                                 <  1:1297138/149‑1 (MQ=255)
                                    cATGCCTCTCTCCTGATGTGCGTTACCGCAACCAGTCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtct                                                                                                                <  1:1196961/149‑1 (MQ=255)
                                     aTTCCTCTCTCCTGATGTGAGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtctc                                                                                                               >  1:1057967/1‑149 (MQ=255)
                                      ttCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCa                                                                                                              <  1:1233712/149‑1 (MQ=255)
                                          tctctcCTGATGTGTGTTACCGCATCAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACtt                                                                                                          >  1:1082060/1‑149 (MQ=255)
                                             ctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtctc                                                                                                       <  1:363205/149‑1 (MQ=255)
                                               ccTGATGTTGGTTACCGCAACAAGGCGGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCAAATAACGAAAAAAAGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGc                                                                                                     <  2:137784/149‑1 (MQ=255)
                                                  gATGTGTGTTACCGCAACAAGGCTGAATAAGGAAGGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTCTTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCCTGAAATCAGACGCCGATATTTCTCAACTTCCCGCCTg                                                                                                  >  2:488721/1‑149 (MQ=255)
                                                   aTGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGc                                                                                                 >  2:190842/1‑149 (MQ=255)
                                                    tgtgtgTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                                                                                <  2:1014385/149‑1 (MQ=255)
                                                       gttttACCGCAACAAGGCTGAATAAGGAACGATACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGCTGCACCGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATc                                                                                             <  1:1135363/146‑1 (MQ=255)
                                                        tgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCGTCa                                                                                            >  1:429055/1‑149 (MQ=255)
                                                         gtTGCCGCAACAAGGCTGAATAAGGAACGAGACAAACGGCCCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGCATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                           <  2:1039204/149‑1 (MQ=255)
                                                         gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                           <  2:167461/149‑1 (MQ=255)
                                                         gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                           >  1:950346/1‑149 (MQ=255)
                                                         gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                           >  1:695792/1‑149 (MQ=255)
                                                         gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                           >  1:1121172/1‑149 (MQ=255)
                                                           aaCCGCAACAAGGCTAAATAATTAACGTGACAAACGCCTCACCCGCCCAGTGACCAATCTCTATGCACGAAAAAAGGGCCGGATGTACTGCTCATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGtt                                                                                         <  2:475652/148‑1 (MQ=255)
                                                                         tGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGt                                                                           <  1:591692/149‑1 (MQ=255)
                                                                          gAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                                                                          >  2:1232506/1‑149 (MQ=255)
                                                                          gAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTCCTCACCTTCTCGCCTGCCATCAATTTGCGTTCGATATGtt                                                                          >  1:534691/1‑149 (MQ=255)
                                                                            aTAAGGTACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCACTAATAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAACAAGACGCCGAGAGTTCACAACTCCTCACCCGCCGACAGTTTACGTTCGATATGTTcc                                                                        >  2:912906/1‑149 (MQ=255)
                                                                            aTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCGATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCTCCTGCCATCAGTTTGCGTTCGATATGTTcc                                                                        <  1:760951/149‑1 (MQ=255)
                                                                             tAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCa                                                                       >  2:541823/1‑149 (MQ=255)
                                                                              aaGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATAGGTTccga                                                                      >  1:589113/1‑147 (MQ=255)
                                                                                ggAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCCGCCATCAGTTTGCGTTCGATATGTTCCAGCg                                                                    >  1:1196977/1‑149 (MQ=255)
                                                                                 gAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGt                                                                   <  2:1207030/149‑1 (MQ=255)
                                                                                   aCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTgc                                                                 >  2:1122537/1‑148 (MQ=255)
                                                                                    cGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGAc                                                                >  2:1419907/1‑149 (MQ=255)
                                                                                     gagaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACa                                                               <  2:1057967/149‑1 (MQ=255)
                                                                                      agaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGGTATGTTCCAGCGTGACAt                                                              >  1:859622/1‑149 (MQ=255)
                                                                                       gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCGTGGCATCAGTTTGCGTTCGATCTGTTTCAGCGTgcactt                                                             >  1:364085/1‑144 (MQ=255)
                                                                                       gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt                                                             >  2:223319/1‑149 (MQ=255)
                                                                                       gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt                                                             >  1:913911/1‑149 (MQ=255)
                                                                                          aatcGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCGGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAttttt                                                          <  1:463833/146‑1 (MQ=255)
                                                                                           aaCGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCCCAACTTCTCGCCGTCCACCAGTTTGCGTTCAATATGCTCCAGCGTGACATTTTTg                                                         >  1:156360/1‑149 (MQ=255)
                                                                                             cGCCTCAACGGCCAAGTGCCCAACCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGt                                                       <  1:432792/149‑1 (MQ=255)
                                                                                                 cTCAACGGCCAAGTGCCCCATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCTTTCGATATGTTCCAGCGTGACATTTTTGGTTTc                                                    <  1:933362/149‑1 (MQ=255)
                                                                                                 cTCAACGGCCAAGTGCCCAATATATATTAAGGATAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc                                                    >  1:1442797/1‑149 (MQ=255)
                                                                                                   cAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCg                                                  <  1:258695/149‑1 (MQ=255)
                                                                                                     acagCCAAGTGCCCAAGCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAATTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGa                                                <  2:979534/146‑1 (MQ=255)
                                                                                                     aCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGa                                                >  2:1203145/1‑149 (MQ=255)
                                                                                                      cGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa                                               <  1:1386663/149‑1 (MQ=255)
                                                                                                         ccAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGa                                            <  1:223319/149‑1 (MQ=255)
                                                                                                          cAAGTGCCCAATCTCTATTAACGAAAAACGGGCCGGATGTACAGCACATCAGGCCCGTTAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTATGAATTCAATGGGGTCCAGCGTGACATTTTTGGTTTCCGGAATGAg                                           <  2:966316/149‑1 (MQ=255)
                                                                                                             gTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCa                                        <  1:1190221/149‑1 (MQ=255)
                                                                                                             gTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGGTTCCGGAATGAGCCa                                        >  2:1215395/1‑149 (MQ=255)
                                                                                                             gTGCCCAAGCTCTATTAACGAAAAAAGGGCCGGATGTACCGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCa                                        <  2:927087/149‑1 (MQ=255)
                                                                                                              tGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAg                                       >  2:179712/1‑149 (MQ=255)
                                                                                                                cccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATTTTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGccgcaa                                     >  2:978377/1‑145 (MQ=255)
                                                                                                                   aaTCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGt                                  <  2:913911/149‑1 (MQ=255)
                                                                                                                        ctATTAACGAAAAATGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATCAGCCAGAAAGTAATGc                             <  1:912851/149‑1 (MQ=255)
                                                                                                                         tATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGAAATGAGCCAGACAGTAATGcg                            >  2:211117/1‑148 (MQ=255)
                                                                                                                         tATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCAGGACTGAGCCAGAAAGTAATGcc                            >  2:872230/1‑149 (MQ=255)
                                                                                                                         tATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGGTTCCGGAATGAGCCAGAAAGTAATGcc                            >  1:380916/1‑149 (MQ=255)
                                                                                                                          aTTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCACAACTTCTCGACTGCCATCCGTTTGCGTTCGATATGTTCCACCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAGGTAGTGacc                           >  2:904447/1‑149 (MQ=255)
                                                                                                                              aCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTAAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaa                       >  2:232784/1‑149 (MQ=255)
                                                                                                                               cGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaaa                      <  2:1429088/149‑1 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGGTGTACAGAACATCCGGCCCGTGGAATCAGACGCCGCTATTTCTAAACTTCTCGCCTGCCCTAAGCTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGCAGTAGCAAGAAACAAAGGCCCCCacac                     >  2:91521/1‑149 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAGTCAGACGCCGATATTTCCCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                     <  2:684022/149‑1 (MQ=255)
                                                                                                                                gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAAc                     >  1:178949/1‑149 (MQ=255)
                                                                                                                                  aaaaaGGGTCTGATGTACAACACATCCGGCCCGTGAAATCAGCCGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGc                   <  2:589113/149‑1 (MQ=255)
                                                                                                                                   aaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTCCCATCAGTTTGCGATCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCa                  >  2:1212933/1‑149 (MQ=255)
                                                                                                                                     aaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAAt                <  2:586170/149‑1 (MQ=255)
                                                                                                                                       gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTGGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGCGTGGACCAGAAAGTAATGCCCACAAACGGAATga              >  1:1110399/1‑148 (MQ=255)
                                                                                                                                       gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATCAGCACCGGCGGGACAGTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACGAACGCAATGt              >  1:698803/1‑149 (MQ=255)
                                                                                                                                       gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGGCCACAAACGCAATGt              >  1:751630/1‑149 (MQ=255)
                                                                                                                                         gCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACACACGCatgtttc            >  1:1328736/1‑143 (MQ=255)
                                                                                                                                          ccGTATGTCCAGCACATCCGGCCCGTGAAATCAGACGCCGTTATTTCTCACCTCCTCCCGTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTGGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa           <  2:226546/149‑1 (MQ=255)
                                                                                                                                          ccGGATGTGCTGTACATCCGGCCGGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa           >  1:806076/1‑149 (MQ=255)
                                                                                                                                          ccGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCa           >  2:215494/1‑149 (MQ=255)
                                                                                                                                            ggATGTATAGCTCATCCGCCCCGTGAAAGCAGACGCCGATATTGCTCAGCTTCTCGCCTGCCATCAGTTTGCGTTCGATAGGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgc         <  2:191499/149‑1 (MQ=255)
                                                                                                                                            ggATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgc         <  2:698488/149‑1 (MQ=255)
                                                                                                                                                 tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAgt    <  2:695792/149‑1 (MQ=255)
                                                                                                                                                 tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGATTGCGTTCGATATGGTCCAGCGTGCCATTTTTGGGTTCCGGCATCAGCCGGAAAGGAATGCCCACAAACGCGATGTTCAGGGCAgt    >  1:976181/1‑149 (MQ=255)
                                                                                                                                                  aCAGCACATCCGGCCCGTGAAATCAGCCGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCATCGCAgtg   <  2:968148/149‑1 (MQ=255)
                                                                                                                                                  aCAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAACCGCAATGTTCAGCCCAgtg   >  1:1269734/1‑149 (MQ=255)
                                                                                                                                                  aCAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAAGGTTCAGCGCAggg   >  1:497094/1‑147 (MQ=255)
                                                                                                                                                   cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTCATGCCCACAAACGCAATGTTCAGCGCAgtgt  >  1:1358118/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGT  >  NC_000913/2980595‑2980889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: