Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,358,324 G→A 5.0% C1201Y (TGC→TAC)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,358,3240GA 5.0% 353.6 / 5.0 122C1201Y (TGC→TAC) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (59/55);  new base A (3/3);  total (63/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-01

Only 100 of 123 total aligned reads displayed.

CTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGC  >  NC_000913/3358182‑3358472
                                                                                                                                              |                                                                                                                                                    
cTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGa                                                                                                                                                >  1:150085/1‑149 (MQ=255)
cTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGa                                                                                                                                                >  2:1181584/1‑149 (MQ=255)
 ttGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaa                                                                                                                                               >  1:1147362/1‑149 (MQ=255)
 cgggCGTACCCCGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTTCATCGaa                                                                                                                                               <  2:1411341/147‑1 (MQ=255)
   ggCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaaa                                                                                                                                             <  2:390464/149‑1 (MQ=255)
     cGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaaca                                                                                                                                           >  1:1429731/1‑149 (MQ=255)
      gTAACTCGTCTGGTGCATCTGATTGGTCGAACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa                                                                                                                                          <  1:1448175/149‑1 (MQ=255)
      gTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCTCATCCAGGTAAGGCACTCTACTGCACCGAAaacaa                                                                                                                                          <  2:652089/149‑1 (MQ=255)
       tAACGCGTCTGGTGGATCTGATTGGTCGAACCGGCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGGCTAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaac                                                                                                                                         <  1:371263/149‑1 (MQ=255)
       tAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCCCCGAAaacaac                                                                                                                                         >  1:695599/1‑149 (MQ=255)
       tAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaacaac                                                                                                                                         >  1:110311/1‑149 (MQ=255)
        gactcGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAcc                                                                                                                                        <  2:772603/145‑1 (MQ=255)
         acacGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATACAGGTCCGGAATTCAACTGCACCGAAAACAAccc                                                                                                                                       >  2:1018889/1‑149 (MQ=255)
              tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCAGCGCCAACGAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTGCTGCACCGAAAACAACCCGCGGt                                                                                                                                  >  2:273902/1‑149 (MQ=255)
              tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAACCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAGGGCACTCTACTGCACCGAAGACAACccgccgg                                                                                                                                  >  1:125385/1‑148 (MQ=255)
                       tCTGATTGGTCGCCCCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCCCCTGCGCCGAAAACAACCCCCCGGTTGATAACg                                                                                                                         >  2:389001/1‑149 (MQ=255)
                       tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACg                                                                                                                         <  1:643935/149‑1 (MQ=255)
                        cTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAACCAGCAGAAGCTCGCGCTGTCGAAGCCACTGGAGACTGCCGAACCGCAACCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAAgg                                                                                                                        >  1:114532/1‑149 (MQ=255)
                          gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGCGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                                                                      >  2:1435110/1‑149 (MQ=255)
                          gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                                                                      >  2:356970/1‑149 (MQ=255)
                           aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCct                                                                                                                     >  1:1032653/1‑149 (MQ=255)
                                 cGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGaa                                                                                                               <  1:79510/149‑1 (MQ=255)
                                  gCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAc                                                                                                              <  1:592180/149‑1 (MQ=255)
                                  gCACCGACCTGAGGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTCCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAc                                                                                                              <  2:1038312/149‑1 (MQ=255)
                                    acctgccTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGTGACTGTCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgc                                                                                                            <  1:563173/144‑1 (MQ=255)
                                     ccGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGAACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCCGCTGCAcgcg                                                                                                           >  1:95541/1‑149 (MQ=255)
                                            gctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCCGGCGCTGGCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgc                                                                                                    <  1:395526/149‑1 (MQ=255)
                                             ctgAAAGGGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgct                                                                                                   <  2:1056052/149‑1 (MQ=255)
                                              cgAAAGGGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGTCGCTGTCGAAGCTGCTGGGGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgctg                                                                                                  <  2:695599/148‑1 (MQ=255)
                                               gAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCCCAGTtgatgc                                                                                                 >  1:1276130/1‑149 (MQ=255)
                                                aaaGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGACCCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCa                                                                                                >  2:1246308/1‑149 (MQ=255)
                                                     gCTGGACGGTTGCACCGCCAAACAGCAGAAGCTGGCCCTGCCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAgg                                                                                           <  1:1123626/149‑1 (MQ=255)
                                                      cTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTACACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGc                                                                                          >  1:913619/1‑149 (MQ=255)
                                                      cTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTACACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGc                                                                                          >  1:860273/1‑149 (MQ=255)
                                                       tGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCg                                                                                         <  1:523469/149‑1 (MQ=255)
                                                               ttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGtt                                                                                 <  1:1382687/149‑1 (MQ=255)
                                                                 cACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAACCAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTCCAACAGGCGAAACCGTTTg                                                                               >  1:188003/1‑149 (MQ=255)
                                                                  aCCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATCACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTCGt                                                                              >  1:510933/1‑149 (MQ=255)
                                                                  aCCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGt                                                                              >  1:309394/1‑149 (MQ=255)
                                                                   ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTTCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTc                                                                             >  1:350924/1‑149 (MQ=255)
                                                                   ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTc                                                                             <  2:624657/149‑1 (MQ=255)
                                                                     gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCCCCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACCGGCGAAACCGTTTGTCGa                                                                           >  1:160150/1‑149 (MQ=255)
                                                                     gCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCAGCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGa                                                                           <  1:1018889/149‑1 (MQ=255)
                                                                      ccAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGAt                                                                          <  2:769600/149‑1 (MQ=255)
                                                                      ccAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGAt                                                                          <  1:977637/149‑1 (MQ=255)
                                                                         aaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGGTGCTGCAACAGGCGAAACCGCTTGTCGAAGaa                                                                       >  2:1123337/1‑148 (MQ=255)
                                                                          aCAGCAGATGCGTGCCCTGTCGAGGCGGCTCGAGATTGCCGAACCGCAGCCAGGTAAGGCACTCTACTGCACCGAAAACACCCCGCCGGTTGATAACGGCCTGCCGAACGCGCAGTTGCTGCAAACGGCGCAACCGTTTGTCGATGatc                                                                      <  2:134339/149‑3 (MQ=255)
                                                                          aCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAgc                                                                      <  1:423864/149‑1 (MQ=255)
                                                                              cagAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCa                                                                  <  2:110311/149‑1 (MQ=255)
                                                                                gacgCTGGCGCGGTGGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCaga                                                                <  2:150466/146‑1 (MQ=255)
                                                                                    cTGGCGTTGTCGAAGCTGCTGGAGACTCCCGAACCGCCTCCAGGCAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCaa                                                            <  2:95541/149‑1 (MQ=255)
                                                                                    cTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCaa                                                            >  2:1284293/1‑149 (MQ=255)
                                                                                    cTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCaa                                                            >  1:1264935/1‑149 (MQ=255)
                                                                                            gTCGCAGCTGCTGGAGACTGCCGACCCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCt                                                    <  2:482910/149‑1 (MQ=255)
                                                                                            gTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCGGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCt                                                    <  2:1095494/149‑1 (MQ=255)
                                                                                                gggctgctGGGGATTGCCGAACCGCATCCGGGTAAGGCACTCTACTGCACCGAAAACACCCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACGGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt                                                <  2:604861/147‑1 (MQ=255)
                                                                                                aaGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt                                                <  2:876291/149‑1 (MQ=255)
                                                                                                   ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAAGAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa                                             >  2:781805/1‑149 (MQ=255)
                                                                                                    tgctgGAGGCTGCCGAACAAAGTCCATGTAGGTCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat                                            <  2:661393/149‑1 (MQ=255)
                                                                                                    tgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAAAAGGCGAGGCCGTGTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGat                                            >  1:1357097/1‑149 (MQ=255)
                                                                                                      ctgGGGACTGCCGAACCGCATGCAGGTAAGGCACGCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGatat                                          <  2:828165/149‑1 (MQ=255)
                                                                                                      ctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAGCAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGaaag                                          >  2:285605/1‑146 (MQ=255)
                                                                                                        ggAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACACCCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCACAGCAAAACCTTCTGGTTCGATATTc                                        <  1:754964/149‑1 (MQ=255)
                                                                                                        ggAGACTGCCGAACCGCAGCCAGGGAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCATCGCAAAACCTTCTGGTTCGATATTc                                        <  1:754968/149‑1 (MQ=255)
                                                                                                            aCTGCCGAACCGCATCCAGGTAAGGCACTCTACTACACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCaa                                    <  2:913619/149‑1 (MQ=255)
                                                                                                            aCTGCCGAACCGCATCCAGGTAAGGCACTCTACTACACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCaa                                    <  2:860398/149‑1 (MQ=255)
                                                                                                             cTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAac                                   >  2:1046665/1‑149 (MQ=255)
                                                                                                              tGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGGGCGCCAGGGCAAAACCTTCTGGTTCGATATTGGCAaca                                  >  1:527656/1‑149 (MQ=255)
                                                                                                               gCCGAATCGCATCCAGGTAAGGCACTTCACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAacac                                 <  1:461493/149‑1 (MQ=255)
                                                                                                               gCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAacac                                 >  1:1145528/1‑149 (MQ=255)
                                                                                                                 cGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccg                               >  1:1249281/1‑149 (MQ=255)
                                                                                                                 cGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTGGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccg                               >  1:92654/1‑149 (MQ=255)
                                                                                                                  gAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccga                              <  2:1032653/149‑1 (MQ=255)
                                                                                                                  gAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccga                              >  1:1186055/1‑149 (MQ=255)
                                                                                                                   aaCCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccgac                             >  2:861096/1‑149 (MQ=255)
                                                                                                                   aaCCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccgac                             >  1:298790/1‑149 (MQ=255)
                                                                                                                   aaCCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccgac                             >  2:715253/1‑149 (MQ=255)
                                                                                                                     ccGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCaga                                                                >  2:434539/1‑112 (MQ=255)
                                                                                                                      cGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGt                          >  2:460189/1‑149 (MQ=255)
                                                                                                                        cATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTc                        >  1:1142824/1‑149 (MQ=255)
                                                                                                                            cAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTc                    <  1:13960/149‑1 (MQ=255)
                                                                                                                               gTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGgc                 <  2:145107/149‑1 (MQ=255)
                                                                                                                                 aaGGCACTCTACTGCACCGAAAACACCCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGgcgc               <  2:902567/149‑1 (MQ=255)
                                                                                                                                   ggCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACCGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGt             >  2:838295/1‑149 (MQ=255)
                                                                                                                                   ggCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGt             <  2:298790/149‑1 (MQ=255)
                                                                                                                                   ggCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGAGCCGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTAGCAACACCGACCGGTCTGTCGGCGCGt             >  1:829857/1‑149 (MQ=255)
                                                                                                                                    gCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTc            >  1:442800/1‑149 (MQ=255)
                                                                                                                                      accctACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGc          <  2:578614/146‑1 (MQ=255)
                                                                                                                                      aCTCTACTGCACCGAAAACACCCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGCTATTCGCAACACCGACCGTTCTGTCGGCGCGTCGc          <  2:878862/149‑1 (MQ=255)
                                                                                                                                       ctctACTGCACCGAAAACACCCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCt         <  1:867893/149‑1 (MQ=255)
                                                                                                                                          tACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTGTc      >  1:243483/1‑149 (MQ=255)
                                                                                                                                          tACTGCACCCGAAACAACCCGCCGTTTGCAAACGGCTTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTc      <  2:926700/149‑1 (MQ=255)
                                                                                                                                           aCTGCCCCGAAAACAACCCGCCGTTTGATAACGGCCTGCGGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCa     <  1:1123337/149‑1 (MQ=255)
                                                                                                                                           aCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCa     <  1:1365444/149‑1 (MQ=255)
                                                                                                                                           aCTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCa     <  1:839578/149‑1 (MQ=255)
                                                                                                                                             tGCACCGCAAACAACCCGCCGTTTGATAACGGCCTGCGGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAgg   <  1:492187/149‑1 (MQ=255)
                                                                                                                                             tGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAACCCTTCTGGTTCGATATTCGCAACACCGACCGGTCTGCCGGCGCGTCGCTTTCAgg   >  2:535841/1‑149 (MQ=255)
                                                                                                                                             tGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAgg   >  2:1426257/1‑149 (MQ=255)
                                                                                                                                             tGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAgg   >  1:76195/1‑149 (MQ=255)
                                                                                                                                              gCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc  >  1:1380231/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGC  >  NC_000913/3358182‑3358472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: