Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,706,969 | C→T | 6.7% | N17N (AAC→AAT) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,706,969 | 0 | C | T | 6.7% | 166.2 / 2.4 | 60 | N17N (AAC→AAT) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Reads supporting (aligned to +/- strand): ref base C (28/27); new base T (2/2); total (31/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 |
ACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAA > NC_000913/1706826‑1707110 | aCTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCCGAAAAAATAAAATCTGGGATTTCAAcggcgg > 2:1327128/1‑149 (MQ=255) cTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTTCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAAAcgcggc > 2:493617/1‑149 (MQ=255) gtaTCTCGTTGCAACCGGTCGCTGAAACACCTGGCTCCTGGAGATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAGGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGcat < 1:383131/147‑1 (MQ=255) cGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCatccatcc > 2:93019/1‑149 (MQ=255) gCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCCTCCATCCCCCg > 1:428601/1‑149 (MQ=255) aaCCGGTCGCAGAAACACCTGACTCCTGGATATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCCGAAAAAATAAAAACTGGGATTTAACCGGCGGCCTCCATCCACCGga > 1:758753/1‑149 (MQ=255) aaaCACCTGACTCCTGGAAATGGGATCTGAACCCCATTCCCGTGCGTATCATTCCCGTGGAACACCAGGCTTAAGTTATTCTCTGCCTTCAGAAAAAATAAAATCCGGAAATTCCACGGCGGCAGCCACCCACCGGAGATCGAAACCCa > 2:888829/1‑149 (MQ=255) aaaCACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCACCGGCGGCATCCATCCACCGGAGATGAAAACCCa > 1:357213/1‑149 (MQ=255) aaaCACCCTACTCCTCGAAAGCGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCa < 2:655834/149‑1 (MQ=255) aCTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGAGGAAAACCCAGTCCAACgg > 2:898131/1‑149 (MQ=255) cTGGAAATGGGATCTGAACACCATTCCCGCGCGTATCCTTCCCGTGGAACACCATGCTTAAGTTACTCTCTGCATTCAGAAAAAATAAAATCTGGGATGTCAACGGCGGCATCCATCCACCGGAGACGAAAAGCCAGTCGCACGGTaga > 2:621781/1‑147 (MQ=255) cTGCAAAGGGGATCTGAACCCCATTCCGGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTaca < 2:1221625/149‑1 (MQ=255) tGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCGATGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTgc > 2:734343/1‑149 (MQ=255) tGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTAGTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAATGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTgc > 2:734241/1‑149 (MQ=255) aTCTGAACACCATTCCCGTTCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCa < 1:528675/149‑1 (MQ=255) tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGt > 1:1232914/1‑149 (MQ=255) tGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGt > 1:1114279/1‑149 (MQ=255) aaCACCATTCCCGTGCGTATCATTCCCGCGGCACACCATGCCTAAGCTACTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGCTGAAAACCCAGCCCCCCCCTCCCCCCCTCCGCCAGGCAc > 2:165341/1‑149 (MQ=255) aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg < 1:93019/149‑1 (MQ=255) aTTCCCGTGCGTATCATTCCCGTGCAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg > 1:1081520/1‑149 (MQ=255) ttCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGCAAAACTAAAATCTGGGATTTCAACGGAGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCACCCTGgc > 1:300723/1‑149 (MQ=255) ccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCa > 1:1193078/1‑149 (MQ=255) gTGCGTATCATCCCCGTGGAACACCATGCTTCATTTTATACCTTCATTTAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCGGTCCAACGGTACACAACTGCGCCAGGGGTACCTGGCGCAgg < 2:305241/149‑2 (MQ=255) ggggTAGCATCCCCGTGGAACACCATGCTTAAGTTACTCCCTGCCTTCCGAAAAAATAAAATCTGGGAGTGCAACGGCGGCAGCCTGGCTCCGGTTTTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCg < 1:345578/146‑1 (MQ=255) tCCCGTGGAACTCCATGCTTAAGTTATGCTCTGCATTCAGAAAAAATAAAATCGGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAtt < 2:822266/149‑1 (MQ=255) tCCCGTGGAACATCATGCTTAAGTGATTCTCTGCATTCTGAAAAAATAAAAGCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAtt < 2:624997/149‑1 (MQ=255) ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACGCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTCGCGCCCCGTTTTGTTGTTcc > 2:424518/1‑149 (MQ=255) ccGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCCGGCGCAGCGGTTTGTTATTcc > 2:1186385/1‑149 (MQ=255) cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCCCGTTTTGTTATTccc > 2:267030/1‑148 (MQ=255) cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa < 1:1327128/149‑1 (MQ=255) gTGGAACACCATGCTTAAGTTATGCCCTGCATTCAGAAAAAAGAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCAc < 1:206896/149‑1 (MQ=255) cacacCATGCTTAAGTTCTTCTCTGCATTCAGAAAAAATAAAAGCTGGGCTTTCAACGGCGGCATCCATCCACCTGAGATGACAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaa < 2:464622/148‑1 (MQ=255) aaCACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaa > 2:181850/1‑149 (MQ=255) acacCCTGCTTAAGTCATTCTCTGCATTCCGAAAAAAAAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGATATGAAAACCCAGTCCAACGGTACTCCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaaa < 1:506545/149‑1 (MQ=255) cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGc < 1:1028876/149‑1 (MQ=255) gCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCTGCGTTTTGTTATCCCACTGAAACAGCata < 1:916648/149‑1 (MQ=255) tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg < 1:181850/149‑1 (MQ=255) aGTTATCCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgc < 1:583481/149‑1 (MQ=255) gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTGCCCCTGGCGAAGCGTGTTGTTATTCCGCGCACACGGCATATTGgcg > 2:845862/1‑149 (MQ=255) gtATTCTCTGCATTCGGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATCGAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc < 2:1086725/148‑1 (MQ=255) ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAATGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCTTTGAAACAGCATATTGGCGCTGAAg < 1:734241/149‑1 (MQ=255) ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAATGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCCCTGAAACAGCATATTGGCGCTGAAg < 1:734343/149‑1 (MQ=255) ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAg < 2:1180027/149‑1 (MQ=255) ctctGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCACCCCCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAg < 1:757399/149‑1 (MQ=255) ctGCATTCAGAAAAAATATCCGCAGGAAGTCCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGt < 2:300723/149‑1 (MQ=255) gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAACCAGGACAGGGGCGGTGAAggggc > 1:1110430/1‑146 (MQ=255) gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGGAGGTGa > 1:12260/1‑149 (MQ=255) ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt > 1:392211/1‑149 (MQ=255) aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg > 1:327897/1‑149 (MQ=255) aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg > 1:735672/1‑149 (MQ=255) aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTtgtg > 1:541394/1‑149 (MQ=255) aaaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGGGAATGAAACGCAGCCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCCCTGAAACAGCATATGGGCGCTGGAGGTGAGTtgtggg > 1:706222/1‑147 (MQ=255) aaaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGGGCAGCGTCTTGTTCTTCCACTGACCCGGCAGGCTGGCGCTGAAGGGGGGGtgtggg > 2:972791/1‑147 (MQ=255) aaaaaaaaatCTGGGATTTCAACGGCGGCATCCATCCCCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGtt < 1:493617/149‑1 (MQ=255) aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTa < 2:1081520/149‑1 (MQ=255) aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTa > 1:584601/1‑149 (MQ=255) ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGc < 1:1226255/149‑1 (MQ=255) aatCTGGGTGTTCGACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTCCCCCTGGCGCAGCGTTTTGTTAGTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCg < 2:210350/149‑1 (MQ=255) tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAGCAGCAAATTGGCGCGGAAGGTGGGTTTCGGGTTGCCGTCggagc > 2:991513/1‑146 (MQ=255) tGGGATTCCAACGGCGGCATCCATCTACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa < 1:898131/149‑1 (MQ=255) aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATaaa < 2:357213/149‑1 (MQ=255) | ACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAA > NC_000913/1706826‑1707110 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |