Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,298,252 T→C 6.3% intergenic (‑131/‑346) adhE ← / → ychE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,298,2520TC 6.3% 177.0 / 2.5 63intergenic (‑131/‑346)adhE/ychEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base C (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

TTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCATCGCTTTTCGCCA  >  NC_000913/1298108‑1298399
                                                                                                                                                |                                                                                                                                                   
ctAGTAACAGCCATAATGCTCTCCTCATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTTCTTCCCCTATTAAAATCAAATGCTTACTCGCGCTTGCACTCACTGTGATTTACt                                                                                                                                                 <  1:1068115/148‑1 (MQ=255)
     aaCGGCCATTATGCTCTCCTGGTAATGTTGAGCTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGAGTTACTAAAag                                                                                                                                            <  2:264123/149‑1 (MQ=255)
      aCAGCCATAATGCTCTCCTTATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAaga                                                                                                                                           <  1:389087/149‑1 (MQ=255)
      aCAGCAATAATGCGCACCTGATAAGGTTAATCTGTTTTAGTACTGCATCTGCCAGTTTACGCGCTTATAGTCAGTTCGGTGCTGCTTTCCTTTACCTATGACAATCAAACGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAaga                                                                                                                                           <  2:666410/149‑1 (MQ=255)
           cATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTa                                                                                                                                      <  1:131547/149‑1 (MQ=255)
             tAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTGACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTaaa                                                                                                                                    <  2:780097/149‑1 (MQ=255)
                  ctctCCTGCTCATGTTACACTTTTTTAGTCAATCATCTGCTCTAATACTAGAGTATAGTCAGGGCGGTGATGCTTTGTTTAACCCATGCACATCAAAAGCTTACCCGCGCGCACACTTACTGTGATTTACTAAAAGAGTTTAAACATTa                                                                                                                               <  2:130774/149‑1 (MQ=255)
                  ctctCCTGATCATGGTAAACTTTTTTAGTAAATCATCTGCTCCAATACGAGAGTATAGTCAGTGCGGGGATGATTGGCTTAACCTATTAAAATCAATAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTa                                                                                                                               <  1:302016/149‑1 (MQ=255)
                  ctctCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTa                                                                                                                               >  1:288202/1‑149 (MQ=255)
                     tcCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTagag                                                                                                                            >  2:814973/1‑149 (MQ=255)
                              tGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAGAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtct                                                                                                                   >  1:1090603/1‑149 (MQ=255)
                              tGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtct                                                                                                                   >  2:541233/1‑149 (MQ=255)
                              tGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtct                                                                                                                   >  2:541062/1‑149 (MQ=255)
                               gTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTAtctc                                                                                                                  >  1:838650/1‑149 (MQ=255)
                               gTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGATTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACGCTCACTGGGATTTACTAAAAGAGTTTAAGCATTAGAGGTATTAtctc                                                                                                                  >  2:431069/1‑149 (MQ=255)
                                ttAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGGGTTTAAACATTAGAGTTATTATatct                                                                                                                 >  2:157869/1‑149 (MQ=255)
                                ttAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATctct                                                                                                                 >  1:410119/1‑149 (MQ=255)
                                      ttttttAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCg                                                                                                           <  2:1340809/149‑1 (MQ=255)
                                       tttttGGTATATGATCTGCTCGGATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGt                                                                                                          <  1:1120247/149‑1 (MQ=255)
                                       tttttAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGt                                                                                                          <  2:787808/149‑1 (MQ=255)
                                        ttttAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTc                                                                                                         <  2:831900/149‑1 (MQ=255)
                                         tttAGTAAATCATCTGCTCGAATACGATAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCa                                                                                                        <  2:1279631/149‑1 (MQ=255)
                                         tttAGGAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCa                                                                                                        <  1:721825/149‑1 (MQ=255)
                                              tAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTcc                                                                                                   >  1:645429/1‑149 (MQ=255)
                                              tAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATCTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTcc                                                                                                   >  1:1140/1‑149 (MQ=255)
                                              tAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATCTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTcc                                                                                                   >  1:1166/1‑149 (MQ=255)
                                                aaTCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAg                                                                                                 >  1:963884/1‑149 (MQ=255)
                                                aaTCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAg                                                                                                 <  1:1235673/149‑1 (MQ=255)
                                                    atcTGCTCGAATAGGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTgg                                                                                             >  2:1010873/1‑149 (MQ=255)
                                                    atcTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTgg                                                                                             <  2:103826/149‑1 (MQ=255)
                                                        gatCGAATACGAGAGGAAAGTCAGTACGTTGATGATTTGCTTAACCTAGGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTa                                                                                         >  2:856165/3‑149 (MQ=255)
                                                                aCGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGAt                                                                                 <  1:1015870/149‑1 (MQ=255)
                                                                  gagaGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTa                                                                               >  1:675483/1‑149 (MQ=255)
                                                                           gTCAGTGCGGTGATGATTTGCTTAACCTATGCAAATAAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTCTTATCTCTAATGCGTCACTTCCCGGTGGCGTAAGCAAGTTTACTCACtctg                                                                      >  2:966950/1‑146 (MQ=255)
                                                                               gTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCCCTTCTGGGt                                                                  >  2:212059/1‑149 (MQ=255)
                                                                                tGCGCTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTa                                                                 <  2:1092510/149‑1 (MQ=255)
                                                                                     tgatgaTTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGGTTACTCACTTCTGGGTACTGAt                                                            >  2:465880/1‑149 (MQ=255)
                                                                                       atgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTa                                                          >  1:883059/1‑149 (MQ=255)
                                                                                       atgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGAACTGATTa                                                          >  2:972639/1‑149 (MQ=255)
                                                                                        tgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTAc                                                         <  1:563115/149‑1 (MQ=255)
                                                                                        tgatTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTCCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTAc                                                         >  1:526489/1‑149 (MQ=255)
                                                                                          atTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGt                                                       >  2:1139422/1‑149 (MQ=255)
                                                                                           tttGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTg                                                      <  1:814973/149‑1 (MQ=255)
                                                                                              gCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATc                                                   <  1:17289/149‑1 (MQ=255)
                                                                                                ttAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTCCTGGGTACTGATTACGTGATCca                                                 >  1:826332/1‑149 (MQ=255)
                                                                                                ttAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAAGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTCACTACGTACTCca                                                 >  2:207203/1‑149 (MQ=255)
                                                                                                   aCCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATCTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaat                                              <  2:1140/149‑1 (MQ=255)
                                                                                                   aCCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATCTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaat                                              <  2:1166/149‑1 (MQ=255)
                                                                                                        tGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCcaaatcaaat                                         <  2:1423851/149‑1 (MQ=255)
                                                                                                            aaTCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAAttttt                                     <  2:781857/149‑1 (MQ=255)
                                                                                                               cAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTCATGCGTCACTTCCAGGTAGCGTAACCAAGATTACTCACTTCTGGGTGATGATTACGTGGTCCAAACAAACTTTTTGCa                                  >  2:157864/1‑149 (MQ=255)
                                                                                                                aaaaGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTCCTGGTGCCCGCTTACCTGATCCGAAACAAAATTTTGCaa                                 >  2:1322619/1‑149 (MQ=255)
                                                                                                                 aaaGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTAATTACGTGATCCAAATCAAATTTTTGCaaa                                >  2:134207/1‑149 (MQ=255)
                                                                                                                  aaGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCaaac                               >  2:137728/1‑148 (MQ=255)
                                                                                                                      ttACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGa                           <  1:321146/149‑1 (MQ=255)
                                                                                                                        aCTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGaca                         >  1:390815/1‑149 (MQ=255)
                                                                                                                        aCTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGaca                         >  2:642180/1‑149 (MQ=255)
                                                                                                                        aCTCGCGCTCACACTCAATGTGATTTACCAAAAGCGTTTACACATTAGAATTAATATCTCTAATGAGTCACTTCCATGTGGCGTAAGCAAGATTACGCACTTCTGTGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGaca                         <  2:347178/149‑1 (MQ=255)
                                                                                                                                tCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAg                 <  2:1445301/149‑1 (MQ=255)
                                                                                                                                  acacTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTGTCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCa               >  2:606826/1‑149 (MQ=255)
                                                                                                                                   cactcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTCCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCAt              <  2:526489/149‑1 (MQ=255)
                                                                                                                                    actcactGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCATc             >  1:852674/1‑149 (MQ=255)
                                                                                                                                              aTTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCATCGCTTTTCGcc   <  2:656331/149‑1 (MQ=255)
                                                                                                                                               tttACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCATCGCTTTTCGCCa  <  2:288202/149‑1 (MQ=255)
                                                                                                                                               tttACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCATCGCTTTTCGCCa  >  2:1138602/1‑149 (MQ=255)
                                                                                                                                               tttACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTAGAAAGCTGACACCTTTCAGCATCGCTTTTCGCCa  <  2:675483/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TTAGTAACAGCCATAATGCTCTCCTGATAATGTTAAACTTTTTTAGTAAATCATCTGCTCGAATACGAGAGTATAGTCAGTGCGGTGATGATTTGCTTAACCTATGAAAATCAAAAGCTTACTCGCGCTCACACTCACTGTGATTTACTAAAAGAGTTTAAACATTAGAGTTATTATCTCTAATGCGTCACTTCCAGGTGGCGTAAGCAAGATTACTCACTTCTGGGTACTGATTACGTGATCCAAATCAAATTTTTGCAAAGCTGACACCTTTCAGCATCGCTTTTCGCCA  >  NC_000913/1298108‑1298399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑