breseq  version 0.32.1  revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,814 Δ1 bp 36.6% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,817 A→T 37.1% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,850 C→T 42.3% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,858 T→A 46.6% intergenic (+89/+19) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,876 C→T 50.6% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase
RA 35,913 C→G 24.5% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase
RA 35,940 G→A 30.5% R204R (CGC→CGT USA300HOU_0026 ← IS431mec transposase
RA 35,970 T→C 60.5% E194E (GAA→GAG USA300HOU_0026 ← IS431mec transposase
RA 35,997 T→C 32.3% T185T (ACA→ACG USA300HOU_0026 ← IS431mec transposase
RA 36,015 C→T 31.0% R179R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,087 A→G 59.9% C155C (TGT→TGC USA300HOU_0026 ← IS431mec transposase
RA 36,119 C→T 33.9% V145I (GTA→ATA)  USA300HOU_0026 ← IS431mec transposase
RA 36,124 G→A 56.7% A143V (GCT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,133 A→G 32.1% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,161 T→C 32.5% I131V (ATT→GTT)  USA300HOU_0026 ← IS431mec transposase
RA 36,174 T→C 30.3% K126K (AAA→AAG USA300HOU_0026 ← IS431mec transposase
RA 36,269 C→T 61.3% A95T (GCA→ACA)  USA300HOU_0026 ← IS431mec transposase
RA 36,315 G→A 59.1% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase
RA 36,378 C→T 87.8% L58L (TTG→TTA USA300HOU_0026 ← IS431mec transposase
RA 36,413 C→T 51.1% V47I (GTC→ATC)  USA300HOU_0026 ← IS431mec transposase
RA 36,444 C→T 46.5% R36R (AGG→AGA USA300HOU_0026 ← IS431mec transposase
RA 36,471 A→G 25.6% S27S (AGT→AGC USA300HOU_0026 ← IS431mec transposase
RA 36,479 T→C 15.9% T25A (ACA→GCA)  USA300HOU_0026 ← IS431mec transposase
RA 36,501 G→A 5.2% A17A (GCC→GCT USA300HOU_0026 ← IS431mec transposase
RA 36,581 C→T 27.1% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 38,412 A→G 7.5% A200A (GCA→GCG ugpQ1 → glycerophosphodiester phosphodiesterase
RA 76,180 C→T 31.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 17.1% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 27.7% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 57.1% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 56.7% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 50.2% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 110,651 C→T 11.2% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 110,660 T→A 12.1% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein
RA 111,512 A→G 22.3% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 21.0% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,560 T→C 11.4% R210R (CGT→CGC USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 117,845 A→T 6.1% E261V (GAA→GTA)  USA300HOU_0115 → possible MFS family major facilitator transporter
RA 293,940 C→T 34.0% intergenic (+57/‑471) USA300HOU_0261 → / → ispD2 zinc (Zn2+)‑binding dehydrogenase/2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
RA 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)
RA 512,643 A→G 48.3% intergenic (+711/‑150) recR → / → rrs1 recombination protein RecR/16S ribosomal RNA
RA 584,121 G→T 100% intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10
RA 707,909 A→T 100% M1M (TTG→ATG) † USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 797,329 G→C 5.0% Q82H (CAG→CAC USA300HOU_0745 → glycine/betaine/carnitine/choline ABC superfamily ATP binding cassette transporter, ABC protein
RA 909,616 C→A 10.4% intergenic (‑539/‑495) ear ← / → USA300HOU_0868 Ear protein/hypothetical protein
RA 973,213 C→A 100% E175* (GAG→TAG)  USA300HOU_0930 ← possible HAD superfamily hydrolase
RA 1,002,200 T→G 100% M173R (ATG→AGG)  mecA2 → competence negative regulator MecA
RA 1,105,994 T→C 100% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,107,008 A→G 4.4% A350A (GCA→GCG pdhD → dihydrolipoyl dehydrogenase
RA 1,196,508 C→A 100% intergenic (+2563/‑1229) ftsQ → / → USA300HOU_1125 cell division protein FtsQ/hypothetical protein
RA 1,391,193 Δ1 bp 7.4% coding (428/1461 nt) alsT → AGCS family alanine or glycine:sodium (Na+) symporter
RA 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,539,250 C→G 100% intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase
RA 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase
RA 1,929,796 T→C 39.6% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 39.8% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 40.0% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 42.7% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 53.3% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,842 Δ1 bp 100% intergenic (‑925/‑243) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 64.8% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 66.3% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 68.9% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,863:1 +T 47.1% intergenic (‑946/‑222) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,864 A→T 21.8% intergenic (‑947/‑221) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 47.3% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865:1 +C 12.1% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 66.7% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 68.0% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 69.8% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 68.0% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 68.1% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 68.2% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 55.4% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 56.0% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 55.6% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 54.5% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 54.0% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 47.8% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 43.4% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,990,921 T→C 6.0% K70K (AAA→AAG glnQ ← glutamate ABC superfamily ATP binding cassette transporter, ABC protein
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  USA300HOU_1861 ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,113,768 T→C 4.0% R79G (AGA→GGA)  USA300HOU_1983 ← hypothetical bacteriophage protein
RA 2,206,678 T→A 5.2% L81* (TTA→TAA)  USA300HOU_2079 → FtsW/RodA/SpoVE family cell division protein
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,337,959 C→T 100% intergenic (‑125/‑119) USA300HOU_2195 ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,343,438 C→T 27.1% intergenic (+114/+250) USA300HOU_2200 → / ← alsD hypothetical protein/acetolactate decarboxylase
RA 2,455,692 Δ1 bp 100% coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,455,698 2 bp→AT 100% coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,470,089 G→A 6.5% G582G (GGC→GGT USA300HOU_2333 ← MFS family major facilitator transporter, multidrug:cation symporter
RA 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein
RA 2,657,572 G→T 10.1% intergenic (‑555/‑127) USA300HOU_2510 ← / → USA300HOU_2511 MarR family transcriptional regulator/possible dioxygenase
RA 2,807,163 C→T 6.6% G487R (GGG→AGG)  USA300HOU_2651 ← hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =68 (0.970)44 (0.910)
+40 bp
32/180 NT 48.4% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA
* ? CP000730 895785 =82 (1.170)5 (0.080) 4/226 NT 7.0% coding (806/822 nt) USA300HOU_0849 ABC superfamily ATP binding cassette transporter, binding protein
?CP000730 = 909740 61 (1.000)intergenic (‑663/‑371) ear/USA300HOU_0868 Ear protein/hypothetical protein
* ? CP000730 2085296 =66 (0.940)5 (0.080) 5/232 NT 8.0% intergenic (‑558/‑400) mapW2/USA300HOU_1944 cell surface protein MapW2/hypothetical protein
?CP000730 = 2128343 56 (0.900)intergenic (+57/+1062) int/USA300HOU_2011 bacteriophage integrase/possible leukocidin subunit
* ? CP000730 = 260086693 (1.330)3 (0.050) 3/238 NT 3.2% intergenic (‑278/+589) USA300HOU_2461/USA300HOU_2462 hypothetical protein/hypothetical protein
?CP000730 = 2600882 95 (1.480)intergenic (‑294/+573) USA300HOU_2461/USA300HOU_2462 hypothetical protein/hypothetical protein