Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 293,940 C→T 34.0% intergenic (+57/‑471) USA300HOU_0261 → / → ispD2 zinc (Zn2+)‑binding dehydrogenase/2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730293,9400CT34.0% 95.2 / 53.0 79intergenic (+57/‑471)USA300HOU_0261/ispD2zinc (Zn2+)‑binding dehydrogenase/2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (22/29);  new base T (10/17);  total (33/46)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAA  >  CP000730/293800‑294080
                                                                                                                                            |                                                                                                                                            
ccGTTAGAAAAAGGCCCGGAGACATTTGATAAATTGGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGc                                                                                                                                              <  2:732514/141‑1 (MQ=255)
ccGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGc                                                                                                                                              >  2:750584/1‑141 (MQ=255)
 cGTTAGAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGTGAATGCCg                                                                                                                                            <  2:155919/141‑1 (MQ=255)
  gTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTGATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCg                                                                                                                                            <  2:266766/141‑1 (MQ=255)
   ttAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGt                                                                                                                                           <  2:675419/141‑1 (MQ=255)
      gAAAAAGTCCCGGATACATTGGATGAATTAGTTAACAAGAAAGAACGATTTGATAAAGCCATGTTTCAGTTATATTAGTCTGCACCCTTGAGGACGAAAACGGTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTg                                                                                                                                        <  2:90891/141‑1 (MQ=255)
      gAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTg                                                                                                                                        <  2:70920/141‑1 (MQ=255)
               ccGGAGACATTTGATTAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGCGGACAAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGa                                                                                                                               <  1:785839/141‑1 (MQ=255)
               ccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAGAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTCATAGCTATGAAAGTGCGAATGACGTCTGGTCTATAGa                                                                                                                               >  1:590346/1‑141 (MQ=255)
               ccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATGGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGa                                                                                                                               >  1:132001/1‑141 (MQ=255)
               ccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGa                                                                                                                               >  1:3824/1‑141 (MQ=255)
               ccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGa                                                                                                                               >  1:235278/1‑141 (MQ=255)
                cGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAATACGAAGGCCGTCTGGTCTATAGAt                                                                                                                              >  2:840117/1‑141 (MQ=255)
                   agaCATTTGATAAATTAGTGAACAAGAAAGAACGATTGGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCTAATGCCGTCTGGTCTATAGATACt                                                                                                                           <  1:533428/141‑1 (MQ=255)
                     aCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTAt                                                                                                                         <  2:293252/141‑1 (MQ=255)
                      cATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATc                                                                                                                        <  2:514975/141‑1 (MQ=255)
                      cATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCGATAgtatctata                                                                                                                        <  2:120706/141‑9 (MQ=255)
                                     ttAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCtt                                                                                                         >  2:711342/1‑141 (MQ=255)
                                      tAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCatcatc                                                                                                        >  1:99664/1‑141 (MQ=255)
                                         cAAGAAAGACAGACTGGATAAAGTCATGTTTACGATTGATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAATTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGaa                                                                                                     <  2:744284/141‑1 (MQ=255)
                                         aaagaaagaACGATTTTATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGaa                                                                                                     <  2:57790/140‑1 (MQ=255)
                                           agaaagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAata                                                                                                   <  1:312796/141‑1 (MQ=255)
                                           agaaagaaCGATTTGATAAAGTCATGTTTAAGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCTAATGTTGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAata                                                                                                   <  1:72328/141‑1 (MQ=255)
                                           agaaagaaCGATTCGATAAAGTCATGTGTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTTGTATAGTTATCGCTAGGAAAGTGCTAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTCCGAata                                                                                                   <  2:462681/141‑1 (MQ=255)
                                             aaagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGACtata                                                                                                 >  1:410986/1‑141 (MQ=255)
                                             aaagaaCGATTTGATAAAGTCATGTTAACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAAtata                                                                                                 >  2:502454/1‑141 (MQ=255)
                                              aagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGAATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCGAAAGCTCCTATCGAAATAATTCATCTTCGAAAATAc                                                                                                >  2:109242/1‑141 (MQ=255)
                                               agaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACg                                                                                               <  1:120866/141‑1 (MQ=255)
                                                 aaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGtt                                                                                             >  1:247616/1‑141 (MQ=255)
                                                 aaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTAGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGtt                                                                                             >  2:763807/1‑141 (MQ=255)
                                                    gATTTGATCAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTAGAGAGACTATCGAAATAATTCATCTTCGAATATACGTTGaa                                                                                          >  2:694423/1‑140 (MQ=255)
                                                    gATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGAt                                                                                          >  2:131842/1‑141 (MQ=255)
                                                     aTTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATa                                                                                         >  1:81798/1‑141 (MQ=255)
                                                          aTAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAg                                                                                    <  1:636808/141‑1 (MQ=255)
                                                            aaaGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGcc                                                                                  <  1:507610/141‑1 (MQ=255)
                                                             aaGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCg                                                                                 <  1:750786/141‑1 (MQ=255)
                                                             aaGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCg                                                                                 <  2:630065/141‑1 (MQ=255)
                                                                 cATGTTAACGATTTATGTGTATGCACCTTTGAGGACGAAAACGCGGGTATAGTTATAGCTATGAAAGTGCGAGTGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGttt                                                                             <  2:78263/141‑1 (MQ=255)
                                                                      ttACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTGTCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtg                                                                        >  2:706233/1‑141 (MQ=255)
                                                                       tACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgt                                                                       >  1:70353/1‑141 (MQ=255)
                                                                        aCGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtg                                                                      >  1:105238/1‑141 (MQ=255)
                                                                         cGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtgt                                                                     >  2:582815/1‑141 (MQ=255)
                                                                           aTTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGa                                                                   <  1:761682/141‑1 (MQ=255)
                                                                            tttATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGGTGATAAATAGCCGGTTTACTTGTGTGaa                                                                  <  1:284757/141‑1 (MQ=255)
                                                                             ttattaGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGaaa                                                                 <  2:731507/141‑1 (MQ=255)
                                                                              tattaGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAat                                                                <  2:268003/141‑1 (MQ=255)
                                                                               actatgaTGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAata                                                               <  2:409917/135‑1 (MQ=255)
                                                                                ttaGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAatat                                                              >  2:682058/1‑141 (MQ=255)
                                                                                 taGTATGCACCTTTGAGGCCGAAAACGCTGGTATAGTTATCGATCTGCGCGTCGGTCTGTTTAAACATCGATAGATACTATCGATTTTACGTGTCTGCTAATATACGTTGATAAATAGCCGGTTTACTTGTGTTAAATATg                                                             <  2:590346/141‑1 (MQ=255)
                                                                                    tATGCACCTTTGAGGACGAAATCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATATGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCtt                                                          <  2:484461/141‑1 (MQ=255)
                                                                                        cACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGa                                                      <  2:782134/141‑1 (MQ=255)
                                                                                           cTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATAATCTTGTGAATc                                                   >  1:270812/1‑141 (MQ=255)
                                                                                            tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATAGCTTGATTAAGAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCg                                                  >  1:646203/1‑141 (MQ=255)
                                                                                            tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCg                                                  >  2:276987/1‑141 (MQ=255)
                                                                                            tttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATATCGGCTGTTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCTCGATTACTTGTGTGAAATATGCTTGTGAATCg                                                  >  2:12716/1‑141 (MQ=255)
                                                                                            tttGAGGACGAAAACGCGGGTATAGTTATAGCTATGAAAGTGCGAAGGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTGTACTTGTGTGAAATATGCTTGTTaagtg                                                  >  2:731076/1‑138 (MQ=255)
                                                                                             ttGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCgg                                                 <  2:46495/141‑1 (MQ=255)
                                                                                              tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                                >  2:479673/1‑141 (MQ=255)
                                                                                                aGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttg                                              >  1:282061/1‑141 (MQ=255)
                                                                                                 ggACGAAATCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGTTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttgt                                             <  1:291566/141‑1 (MQ=255)
                                                                                                  gaagAAAACGCTGGGATAGTTATAGCTATGAAAGTGCGAATGCCGGCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttgtt                                            <  1:25692/138‑1 (MQ=255)
                                                                                                  gACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGTATCGgttgtt                                            >  2:204995/1‑141 (MQ=255)
                                                                                                  gACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTAGAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttgtt                                            <  1:192884/141‑1 (MQ=255)
                                                                                                   aCGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGACTGTCGTCTGGTCTGTAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGttt                                           <  1:64202/141‑1 (MQ=255)
                                                                                                   aCGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGttt                                           <  1:440238/141‑1 (MQ=255)
                                                                                                   aCGAAAACGCTGGTATAGTTATAGCTATGAAAGTCCGAATGTCGTCAACTCTATGGATACTATCGAACTAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGttt                                           <  1:64209/141‑1 (MQ=255)
                                                                                                    cGAAAACGCTTGTATAGTTATAGCTAGTAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGtttt                                          <  2:834796/141‑1 (MQ=255)
                                                                                                     gAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTg                                         <  1:306693/141‑1 (MQ=255)
                                                                                                        aaCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAt                                      >  1:664751/1‑141 (MQ=255)
                                                                                                         aCGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCAtt                                     <  2:282061/141‑1 (MQ=255)
                                                                                                                   aGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGCGTGAAATATGCTTGTGAACCGGTTCTTTTCCATTTTGTATACtt                           <  1:458995/141‑1 (MQ=255)
                                                                                                                       ataGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTaaaa                       <  2:492783/141‑1 (MQ=255)
                                                                                                                             aTGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATgagag                 >  1:348540/1‑140 (MQ=255)
                                                                                                                             aTGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTCGTGTGAAGTATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGAt                 >  1:318222/1‑141 (MQ=255)
                                                                                                                              tGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATg                >  1:472870/1‑141 (MQ=255)
                                                                                                                              tGAAAGTGCGAATGCCGTCTGGTCGATCGATTCTATCGAAAGAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATg                <  1:26358/141‑1 (MQ=255)
                                                                                                                                  aGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTATACTTGTGAGAAATATGCTTGTGAATCGGTTATTTTGCATTTTGTATACTTAAAATGAGATGGCaa            >  2:682023/1‑141 (MQ=255)
                                                                                                                                       gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATAttt       <  1:192462/141‑1 (MQ=255)
                                                                                                                                       gAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGACTATCCGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATAttt       <  1:23513/141‑1 (MQ=255)
                                                                                                                                         aTGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGa     <  1:682058/141‑1 (MQ=255)
                                                                                                                                          tGCCGTCTGGTTTATAAATACGCTTGAATTATTTCATCTTCGAATATACGTTAAAAGATTGTCGGTTTACGTTTGCGAAATGTGATTGTGAATCGGTGTTTTTGCATTTTGTATACTTTATATGAGATGTCAATATTTGAt    <  2:330151/141‑1 (MQ=255)
                                                                                                                                            ccGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATTTACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGATTCGGTTGTTTTGCATTTTGTTTATTTAAAATGAGATGGGAATATTTGAtgt  >  2:456305/1‑139 (MQ=255)
                                                                                                                                            ccGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATaa  >  1:410459/1‑141 (MQ=255)
                                                                                                                                            ccGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATaa  >  1:390094/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAA  >  CP000730/293800‑294080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: