Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,206,678 T→A 5.2% L81* (TTA→TAA)  USA300HOU_2079 → FtsW/RodA/SpoVE family cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,206,6780TA 5.2% 237.8 / 2.5 76L81* (TTA→TAA) USA300HOU_2079FtsW/RodA/SpoVE family cell division protein
Reads supporting (aligned to +/- strand):  ref base T (41/31);  new base A (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CTTATTAACTCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCATGAA  >  CP000730/2206539‑2206813
                                                                                                                                           |                                                                                                                                       
cTTATTAACTCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAATCATTATACAtattta                                                                                                                                        <  2:234024/141‑1 (MQ=255)
cTTATTAACTCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACAtattta                                                                                                                                        >  1:516136/1‑141 (MQ=255)
    ttaACTCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACGTTTTAGTTGCAATTTTTGCAGGTCTCAGAATGTTTAATTCACCTAAAAAGATTAAACATTATACATATATATTGt                                                                                                                                    >  2:625846/1‑141 (MQ=255)
    ttaACTCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTGAAAAGATTAACCATTATACATATTTATTGt                                                                                                                                    >  2:530620/1‑141 (MQ=255)
         tCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTc                                                                                                                               >  2:702625/1‑141 (MQ=255)
         tCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTc                                                                                                                               >  2:26438/1‑141 (MQ=255)
         tCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTc                                                                                                                               >  2:368487/1‑141 (MQ=255)
          cGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTACACATTATACATATTTATTGTATTTCt                                                                                                                              <  2:490435/141‑1 (MQ=255)
          cGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCt                                                                                                                              <  1:710787/141‑1 (MQ=255)
          cGGCAATGGGCGGTGGTCAATAAAGTGCTACTTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCt                                                                                                                              <  1:697047/141‑1 (MQ=255)
            gCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATTAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTa                                                                                                                            <  2:333801/141‑1 (MQ=255)
            gCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTa                                                                                                                            >  2:628363/1‑141 (MQ=255)
            gCAATGGGCGGTGGTCAATAAAGTACTAATTTAGGTATCAAACAAATTGTTTATTACATTTTAGGTGCAATTTTTGCAGGTAGCATAATGTTTAATTTAAGTAGAAAACATAAAAATTCTGCATATTTCTTGCATGTCTTa                                                                                                                            >  2:325152/1‑141 (MQ=255)
                 gggCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAAGTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTg                                                                                                                       >  1:403980/1‑141 (MQ=255)
                 gggCGGTGGTCAATACAGTGCCAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCtt                                                                                                                       >  1:436166/1‑140 (MQ=255)
                  ggCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGt                                                                                                                      >  1:119704/1‑141 (MQ=255)
                    cGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCt                                                                                                                    >  1:745558/1‑141 (MQ=255)
                    cGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTAATTGTATTTCTTAATCTGTCt                                                                                                                    >  1:142339/1‑141 (MQ=255)
                    cGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTAATTGTATTTCTTAATCTGTCt                                                                                                                    >  1:740575/1‑141 (MQ=255)
                     ggtggtCCATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCta                                                                                                                   <  2:13276/141‑1 (MQ=255)
                      gtggtCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCtat                                                                                                                  <  1:152372/141‑1 (MQ=255)
                        ggtCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCtatta                                                                                                                <  1:307935/141‑1 (MQ=255)
                         gtCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCtattat                                                                                                               >  1:740918/1‑141 (MQ=255)
                           cAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTAttatta                                                                                                             <  2:693744/141‑1 (MQ=255)
                                   tGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCtt                                                                                                     >  1:287899/1‑141 (MQ=255)
                                       aaTTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTc                                                                                                 <  1:477704/141‑1 (MQ=255)
                                         tttCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGt                                                                                               <  1:176093/141‑1 (MQ=255)
                                         tgggggTACCAGACTAGTTTTCTTTTATATTTTAAGTGCAATTTTTTCACGTTTCATCTTGTTAAAAAAAAAAAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGt                                                                                               <  2:621310/137‑1 (MQ=255)
                                           tCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTa                                                                                             >  1:379501/1‑141 (MQ=255)
                                            cGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCTTCATGTTTATTTTACCTAAAAAGATTAAAAATAAAACATTTATATTGTAATTCTTAATCTGCCTATTTTTAATAGGGTTTTTTGTTAt                                                                                            >  2:163050/1‑141 (MQ=255)
                                            cGGTATCAGACAAATTTTTTATTACATATTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTCTTAATAGGCTTGCTCGTTAt                                                                                            >  2:17585/1‑141 (MQ=255)
                                             ggTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATATGTCTATTGTTAATAGGCTTGCTCGTTAtt                                                                                           >  2:61911/1‑141 (MQ=255)
                                               tATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTCACTCTGTCTATTATTAATAGGCTTGCTCGTTATTcc                                                                                         <  2:737201/141‑1 (MQ=255)
                                                 tCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTg                                                                                       <  1:743785/141‑1 (MQ=255)
                                                 tCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTg                                                                                       >  1:581820/1‑141 (MQ=255)
                                                 tCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTg                                                                                       >  2:678219/1‑141 (MQ=255)
                                                   aGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTTTTCCTGat                                                                                     >  1:256432/1‑140 (MQ=255)
                                                          ttttttATTACATTTTAGGTGCAATTTTTGCAGGTATCATCTTGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTATTAGGCTTGCTCGTTATTCTTGAGTcacttt                                                                              >  2:655670/1‑138 (MQ=255)
                                                          ttttttATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAATAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGATATTCCTGATTcagctt                                                                              >  2:560955/1‑140 (MQ=255)
                                                          ttttttATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcaccta                                                                              <  1:368487/141‑1 (MQ=255)
                                                          ttttttATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcaccta                                                                              <  1:702625/141‑1 (MQ=255)
                                                            ttttATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTAATTGTATTTCTTAATCTGTCTATTATTAATCCGCTTGCTCGTTATTCCTGAGTcacctatt                                                                            <  2:142339/141‑1 (MQ=255)
                                                            ttttATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTAATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctatt                                                                            <  2:740575/141‑1 (MQ=255)
                                                              ttattaCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattac                                                                          <  1:805957/141‑1 (MQ=255)
                                                                attaCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTTTTCCTGAGTcacctattacac                                                                        >  1:674824/1‑141 (MQ=255)
                                                                 ttaCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacacc                                                                       <  1:26438/141‑1 (MQ=255)
                                                                  taCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacacct                                                                      <  2:702885/141‑1 (MQ=255)
                                                                  taCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacacct                                                                      <  2:145665/141‑1 (MQ=255)
                                                                  cccaggttAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacacct                                                                      <  1:392004/135‑1 (MQ=255)
                                                                   aCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacacctt                                                                     >  1:428223/1‑140 (MQ=255)
                                                                   aCATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacaccta                                                                     <  1:668572/141‑1 (MQ=255)
                                                                    cATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTCTTAATAGGCTTGCTCGTTATTCCTTAGCtacctattacacctat                                                                    >  1:7965/1‑141 (MQ=255)
                                                                    cATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTCATTCCTGAGTcacctattacacctat                                                                    <  2:519591/141‑1 (MQ=255)
                                                                     aTTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTcacctattacacctatt                                                                   >  1:817468/1‑141 (MQ=255)
                                                                          aGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAACGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCGTGAGACACCTATTAAACCTATTGTCaa                                                              >  1:804842/1‑141 (MQ=255)
                                                                          aGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGCGTCACCTATTACACCTATTATCaa                                                              >  1:60789/1‑141 (MQ=255)
                                                                           ggTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAAt                                                             <  1:492526/141‑1 (MQ=255)
                                                                                       gCAGGTAACAATATGTTTATTTCACCTAAAAAGATAAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGTTCGTTATTCCTGAGTCACCTATTACACCTATTATTAATGGTGCCAAAAGt                                                 <  1:556581/141‑1 (MQ=255)
                                                                                         aGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTg                                               <  1:170762/141‑1 (MQ=255)
                                                                                           gTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCAATTATCAATGGTACCAAAAGTTGGt                                             >  1:627194/1‑141 (MQ=255)
                                                                                             atcatcATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTac                                           <  1:5980/141‑1 (MQ=255)
                                                                                                atcatTTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACg                                        <  1:187722/141‑1 (MQ=255)
                                                                                                  catGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTACTTAATCTTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACCCGtt                                      >  1:397136/1‑141 (MQ=255)
                                                                                                      tttATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCCATGTTGCCAAAAGTTGGTACACGTTTGGc                                  >  1:323007/1‑141 (MQ=255)
                                                                                                          tttCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTa                              >  2:611505/1‑141 (MQ=255)
                                                                                                            tCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATc                            >  1:2634/1‑141 (MQ=255)
                                                                                                            tCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATc                            <  2:817468/141‑1 (MQ=255)
                                                                                                            tCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAACCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATc                            <  2:726519/141‑1 (MQ=255)
                                                                                                               ccTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACCCGTTTGGCCCTATCAGt                         >  1:713206/1‑141 (MQ=255)
                                                                                                               ccTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGt                         >  2:427904/1‑141 (MQ=255)
                                                                                                                            aaCATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTg            >  2:543430/1‑141 (MQ=255)
                                                                                                                             aCATTATACATATTTATTGTATTTCTTAATCTGTTTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGa           <  2:40276/141‑1 (MQ=255)
                                                                                                                                  ataCATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCa      >  1:75564/1‑141 (MQ=255)
                                                                                                                                  ataCATATTTATTGGATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCa      >  1:457351/1‑141 (MQ=255)
                                                                                                                                     cATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCTGTATTCAGCCATCTGAATTCATGa   <  1:611505/141‑1 (MQ=255)
                                                                                                                                     cATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCATGa   >  2:828031/1‑141 (MQ=255)
                                                                                                                                      atatTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCATGaa  >  1:289204/1‑141 (MQ=255)
                                                                                                                                      atatTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGCTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCATGaa  >  2:68769/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CTTATTAACTCGGCAATGGGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGTGCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATATTTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCTATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAGCCATCTGAATTCATGAA  >  CP000730/2206539‑2206813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: