BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	61.3%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	61.3%	336.0 / inf	837	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (160/163); new base T (235/273); total (398/439)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 845 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

 gcgtccTTCGGTGAGGGTGCTTGATGTGCATTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTGGATAAACGCATATGCGGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                             <  1:101720/137‑1 (MQ=11)

  cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAt                                                                                                                                            >  1:708302/1‑141 (MQ=32)

   tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                                                                                                                           <  2:750254/141‑1 (MQ=17)

   tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                                                                                                                           >  1:743018/1‑141 (MQ=32)

    tccTTTGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  1:259596/140‑1 (MQ=12)

    aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          <  2:782951/141‑1 (MQ=17)

     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         <  2:375299/141‑1 (MQ=17)

     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                                                                                                                         >  1:98386/1‑141 (MQ=32)

       ttCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                                                                                                                       <  2:113869/141‑1 (MQ=255)

          gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTGCGCAACCAAATATCTAATGTATGTACGTCTGCATCAATGGcc                                                                                                                                    >  2:619896/1‑140 (MQ=12)

          gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCGAATATCTAATGTATGTCCGTCTGCATCAATGGcc                                                                                                                                    >  2:311809/1‑140 (MQ=17)

          gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCa                                                                                                                                    >  2:485050/1‑141 (MQ=32)

               aGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAGTATCTAATGTATGTCGCTCTGTATCAATGGCACGatt                                                                                                                               >  1:782052/1‑140 (MQ=11)

               aGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACAAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGCATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTCTGTCACTCTGTATCAATGGCACGata                                                                                                                               >  1:137907/1‑141 (MQ=11)

                       taTCTGCAACTATCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc                                                                                                                       <  1:330142/140‑1 (MQ=11)

                       gcgcTGTTATTTCCTTTTGAGGTTTCACAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                                                                                                                       <  2:207211/138‑1 (MQ=11)

                        ttCTGCAACTTCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCCTATGCTGAATGATTATGCTATTGCTTACGCGAGCAATTACCTAATGTATGTACCTCTGTATCAATGGCACGATATAAATAGCt                                                                                                                      <  2:707/140‑1 (MQ=11)

                         tCTGTAATTACCTTTTGATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                     <  1:478778/141‑1 (MQ=17)

                                ctACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              <  1:795473/140‑1 (MQ=12)

                                  aCCTTTTGCGGTTTACAAAATTGTTTAATGAGACTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            <  1:414353/141‑1 (MQ=12)

                                  aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            >  1:721524/1‑141 (MQ=32)

                                   ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           >  2:676035/1‑141 (MQ=17)

                                   ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCGCCCTTTTCCtt                                                                                                           >  2:256266/1‑141 (MQ=255)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAATTATCTAATGTATGTACCTCTGTATCAATGGCACGCTATAAATCGCCACATTTTCCttg                                                                                                          >  2:332922/1‑140 (MQ=11)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  1:693093/141‑1 (MQ=32)

                                    cTTTTGAGGGTTACCAATTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  1:119237/141‑1 (MQ=17)

                                     ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                         >  1:748651/1‑141 (MQ=17)

                                      tttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCttttt                                                                                                        >  2:55169/1‑140 (MQ=17)

                                        tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGATTCCGCAACCAAATTTCTGTTGGAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                      <  1:401333/141‑1 (MQ=11)

                                        tGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTCTCTCGTTACTTACGCATCCAAATAAATAATGGGTGACCCTCTGTATCAATGGCACGTTATAAAAAGCGCCATTTTCCTTTTAtt                                                                                                      >  2:716273/1‑141 (MQ=11)

                                         gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCCTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGACCCATTATccatataaat                                                                                                     >  2:118879/1‑133 (MQ=11)

                                          aggcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGACACGATATAAATAGCTCCATTTTCCTTTTAtttt                                                                                                    >  2:268809/5‑141 (MQ=12)

                                           ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   <  1:757978/141‑1 (MQ=17)

                                                tccAAATTCTTTAATGAGTCGTTTGATAGACGTATATGCTGACTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGAtata                                                                                              <  1:610896/140‑1 (MQ=11)

                                                aacAAATTGTTTCATGAGTCGTTTTATAATCGTATATGCTGAATCACTATCTCGTTTCTTTCGCAACCAAATATCGAATGTATGTCCCTCTGTATCCATGTCACGATATAAATAGCTCTATTTTCCTTTTATTTTGAtata                                                                                              <  2:648629/139‑1 (MQ=11)

                                                    aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCATTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTCTCAATGGCACGATATAAATAGCCCCATTTTCCTTGTATCTTGATATACGtc                                                                                          >  2:325467/1‑141 (MQ=11)

                                                          ttAACGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatca                                                                                    >  1:569714/1‑141 (MQ=12)

                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                  >  1:59313/1‑141 (MQ=17)

                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                  <  1:834907/141‑1 (MQ=32)

                                                            aaTGAGACGTGTTAAAAACGCATAGGCTGAACGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                  <  1:21318/141‑1 (MQ=11)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATa                                                                                 <  1:634987/141‑1 (MQ=32)

                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  1:450927/141‑1 (MQ=12)

                                                              ggataCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  2:139082/137‑1 (MQ=12)

                                                               gagaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATACACGTCTCATCAATACg                                                                               <  1:465247/141‑1 (MQ=12)

                                                                    gTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                          <  2:663704/141‑1 (MQ=12)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                          <  1:17830/141‑1 (MQ=17)

                                                                    gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAAGTACGTCTCATCAATACGCCaat                                                                          >  1:349532/1‑139 (MQ=17)

                                                                    gTTGGATAAACGCAGATGCTGAATGATTATCTCGGTTCTCACGATACGAACTATCTAAGGTCTGTCCCTCGGTCTCAATAGCAGGATATAAATAACACTATTTTCCTTCTGTTTTGATATACGACTCATCAATACGCCAtt                                                                          >  2:51806/1‑141 (MQ=2)

                                                                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTAGGTATCATGGATActagttgtt                                                                        >  1:32206/1‑133 (MQ=11)

                                                                          tAACCGCATATGCTGAATGATTATCGCGTTGATCACGAAACCATTTATCGGATGTATGTTCCACTCTATTAATGGTTTGACAAAAATAGGTCCATTTACCTTGTACTTTGTGAGACGTCTCATCAATACTCCATTTGtaat                                                                    <  2:240178/141‑1 (MQ=2)

                                                                          tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                    <  1:771825/141‑1 (MQ=12)

                                                                          tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                    <  2:729035/141‑1 (MQ=17)

                                                                           aaaCGCATATGATGAATAATTATCTCGTGACTTACGCAACCAAATAGCTAATGTATGACCCTCTGCATCAATGGCACGATAGAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                   <  1:88998/141‑1 (MQ=11)

                                                                              cGCATATGGTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                <  1:223482/141‑1 (MQ=255)

                                                                              cGCATATGCTTAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                <  1:280417/141‑1 (MQ=255)

                                                                              cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGc                                                                >  2:154985/1‑141 (MQ=35)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  1:308163/1‑141 (MQ=25)

                                                                                    tGCTGAATGATTATCGCGTAGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          >  1:624757/1‑141 (MQ=255)

                                                                                       tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       <  1:298633/141‑1 (MQ=255)

                                                                                        gAATGATTATCTCGTTGCTGACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                      <  2:478393/141‑1 (MQ=255)

                                                                                         aaTGATTATCTCTTTGCTTACGCAACCAAATATTTAATGTATGCCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                     <  2:56882/141‑1 (MQ=14)

                                                                                         aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATgtt                                                     >  2:403410/1‑139 (MQ=255)

                                                                                         aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTATTATGCt                                                     <  1:526094/141‑1 (MQ=38)

                                                                                           tGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCttt                                                   >  1:552170/1‑141 (MQ=255)

                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTtttt                                                  >  1:494671/1‑141 (MQ=255)

                                                                                            gATTATCTCGAAGCTGGCGAGACGACATATCTACTGTATGTCCATAAGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAGGTACGTCGCATCAATCCGCCATTTGTAATAAGCTCTTTTTTGCgttt                                                  >  2:832062/1‑141 (MQ=2)

                                                                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTAGGTCCATCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATATGCTTTTTTagtattttt                                                 >  2:86803/1‑133 (MQ=11)

                                                                                             aTTAGCTCGTTGCTTACGCACCCAATTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                 <  2:1742/141‑1 (MQ=255)

                                                                                              ttATCTCGTTGCTTAAGCAACCACACACTTATTATATGTCCCTCTGCATCAATGGCACGATATAAATGGACCCATTTTCATTTTATTTTGATGTACGTCTCCTCAATACACCATTCGTGATAAGCttttttctcgtttttt                                                >  2:807409/1‑131 (MQ=2)

                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  2:503537/1‑141 (MQ=255)

                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTAATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  1:161173/1‑141 (MQ=255)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTCTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  1:81154/141‑1 (MQ=255)

                                                                                                 cctcctcggttccGCAACTAAATATTTAATGTTTGTCCCTCCGCATCATTGTCACGAGATAAATAGCTCCATTTTCCTTTTATTTGGATGTACGTCTCATCATTACGTCATTGGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:438289/129‑1 (MQ=14)

                                                                                                  ctcGTTGCTTACGCAACGAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  2:281376/141‑1 (MQ=255)

                                                                                                      ttgcttcgcaaaCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAt                                        <  2:416409/131‑1 (MQ=14)

                                                                                                        gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAACttt                                      >  2:737699/1‑141 (MQ=255)

                                                                                                          ttACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                    <  1:68267/141‑1 (MQ=255)

                                                                                                           tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAACCTTTTTTATGTTTTTTCTTCCAAATTTTAt                                   >  1:511601/1‑141 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:127959/141‑2 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGTTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:649588/141‑2 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  2:686545/141‑2 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACATCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATat                                 >  2:187916/1‑140 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCATCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:245442/141‑2 (MQ=255)

                                                                                                             cGCAACCAAATATCCAATGTAGGTCCCTCTGTAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:378004/141‑2 (MQ=14)

                                                                                                              gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                <  2:637526/141‑3 (MQ=255)

                                                                                                               cAACCAAATATCTTATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACaa                               <  2:129332/141‑1 (MQ=255)

                                                                                                                   cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                           >  1:664464/1‑141 (MQ=255)

                                                                                                                          ttAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  2:54074/140‑1 (MQ=25)

                                                                                                                          cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  1:432054/141‑1 (MQ=255)

                                                                                                                           tAATTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                   <  1:195442/141‑1 (MQ=255)

                                                                                                                             atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                 >  2:299860/1‑141 (MQ=255)

                                                                                                                                tagttCCCTCTGCATCAATGGCACGTTATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt              <  2:167803/137‑1 (MQ=255)

                                                                                                                                 ttttcctgctGCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACATTTGGGGCATATTCTTg             <  2:457263/133‑1 (MQ=11)

                                                                                                                                 ttctCCCTCTGCATCAATGGCACGATATAAATTGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCGAGTGTGATATAAAATTGGGGCATATTCTTg             <  2:348139/138‑1 (MQ=11)

                                                                                                                                 atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg             >  2:389491/1‑141 (MQ=255)

                                                                                                                                   gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa           <  2:769975/141‑1 (MQ=25)

                                                                                                                                    tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc          >  1:361980/1‑141 (MQ=21)

                                                                                                                                     cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCCAATTTGGTATAAAATTGGGGCATATTCTTGAAcc         >  1:821326/1‑141 (MQ=11)

                                                                                                                                      ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc        >  2:596029/1‑141 (MQ=255)

                                                                                                                                        tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGACCCCaa      >  2:226231/1‑141 (MQ=11)

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`