Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 31.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT31.7% 214.6 / 81.8 113intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (43/32);  new base T (12/23);  total (57/56)
Fisher's exact test for biased strand distribution p-value = 3.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 114 total aligned reads displayed.

GAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76041‑76319
                                                                                                                                           |                                                                                                                                           
gAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCt                                                                                                                                            <  1:463361/141‑1 (MQ=255)
   tttAATATAACTAAGATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCtata                                                                                                                                         <  1:488584/141‑1 (MQ=255)
      aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                      <  1:185622/141‑1 (MQ=255)
               aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                             <  1:551900/141‑1 (MQ=255)
                 tatTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACa                                                                                                                           <  1:775324/141‑1 (MQ=255)
                  atTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaa                                                                                                                          >  2:745794/1‑141 (MQ=255)
                    tgctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaa                                                                                                                        <  1:837587/141‑1 (MQ=255)
                     gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGCATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAGATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACtaaaa                                                                                                                       >  2:625954/1‑141 (MQ=255)
                     gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaa                                                                                                                       <  1:515302/141‑1 (MQ=255)
                        gcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGa                                                                                                                    <  2:708824/141‑1 (MQ=255)
                         cACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAg                                                                                                                   >  2:199717/1‑141 (MQ=255)
                             tGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                               <  2:516419/141‑1 (MQ=255)
                              gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAGTTTTAACAAAAAGGAGtctgc                                                                                                              >  2:575973/1‑139 (MQ=255)
                               tATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAACATAAAGTATAATTTTAAAAAAACGGAgccacct                                                                                                             <  2:830691/141‑7 (MQ=255)
                               tATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCAAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTCTAGTATAATTTTAACaaaccgaagtcatcc                                                                                                             >  2:530832/1‑129 (MQ=39)
                                 tCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGt                                                                                                           >  1:470894/1‑141 (MQ=255)
                                     ttcgAACAGCTAACCAAAGAGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAACATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGa                                                                                                       <  1:352905/138‑1 (MQ=255)
                                     atcgAACAGCTAACCAAAGTGGTAGTAAACCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGa                                                                                                       <  1:714800/138‑1 (MQ=255)
                                      tagaacgtagaaCCAAAGTGTTAGTAATCCAGGGATTACTTCCATATAAACAAGAAATTTAATAATTATAGCAAAATATAGGGTCTGTTGCAAAGTTGTATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGaa                                                                                                      <  2:712250/131‑1 (MQ=19)
                                        gAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGGATAATTTTAACAAAAAGGAGTCTACTGTATGAACt                                                                                                    >  1:602899/1‑141 (MQ=255)
                                          aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                  >  2:335194/1‑141 (MQ=255)
                                              cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                              >  1:684099/1‑141 (MQ=255)
                                              cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                              >  2:742348/1‑141 (MQ=255)
                                              cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAACGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAATTTAACAAAAAGGAATCTTCAGTAAGAACTATTTCa                                                                                              >  2:555218/1‑141 (MQ=37)
                                                 aCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGat                                                                                           >  1:553973/1‑141 (MQ=255)
                                                    aaaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                        >  2:272463/1‑141 (MQ=255)
                                                       gtgtTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAAc                                                                                     >  2:782921/1‑141 (MQ=255)
                                                            aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                <  1:732705/141‑1 (MQ=255)
                                                              tAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGATGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCACATATAAAAAAtttca                                                                              >  2:411617/1‑139 (MQ=255)
                                                                    aGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGa                                                                        <  1:812832/141‑1 (MQ=255)
                                                                      ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                      >  2:68422/1‑141 (MQ=255)
                                                                       gAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                     >  1:39840/1‑141 (MQ=255)
                                                                           aCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTGTc                                                                 >  1:448946/1‑141 (MQ=255)
                                                                           aCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATc                                                                 >  1:339528/1‑141 (MQ=255)
                                                                            cTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCa                                                                <  1:555182/141‑1 (MQ=255)
                                                                             tAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAc                                                               <  2:144003/141‑1 (MQ=255)
                                                                             tAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGACTTCTGTATGAACTATTTCAGATATAAAAAATTTAACAAGGATGTTATCAc                                                               >  1:723038/1‑141 (MQ=255)
                                                                              aaCACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAAGATGTTGTCaat                                                              >  1:733303/1‑139 (MQ=255)
                                                                               acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                             <  2:127380/141‑1 (MQ=255)
                                                                                    aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        >  2:261270/1‑141 (MQ=255)
                                                                                     aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                       >  1:352501/1‑141 (MQ=255)
                                                                                       gCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGtt                                                     <  1:127183/141‑1 (MQ=255)
                                                                                        cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                    >  1:293729/1‑141 (MQ=255)
                                                                                         aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                   <  1:569100/141‑1 (MQ=255)
                                                                                           ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGct                                                 <  1:745794/141‑1 (MQ=255)
                                                                                                      atTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGa                                      >  2:796977/1‑141 (MQ=255)
                                                                                                        tataGCAAAATATAGGTTCTGTTGAAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGata                                    <  2:347322/141‑1 (MQ=255)
                                                                                                            cagaaaatgggggtCTTTTGCAAAGTTGAATGTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                >  2:830689/14‑141 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               >  1:366153/9‑141 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAAATATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               >  2:90332/9‑141 (MQ=1)
                                                                                                              agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                              <  1:39187/134‑1 (MQ=11)
                                                                                                              agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTGAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                              >  2:133860/8‑141 (MQ=1)
                                                                                                              aaaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTACGATATGCAtt                              >  1:15953/1‑141 (MQ=255)
                                                                                                              aaaaTATAGGTTATGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAACGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                              <  2:447294/141‑1 (MQ=255)
                                                                                                               gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             <  2:114698/135‑1 (MQ=2)
                                                                                                               attaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             <  2:825438/135‑1 (MQ=255)
                                                                                                               aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAGGATATGCATTg                             >  1:680847/1‑141 (MQ=255)
                                                                                                               aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTg                             >  1:188019/1‑141 (MQ=255)
                                                                                                                ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTATCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  2:616653/136‑1 (MQ=11)
                                                                                                                ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTgg                            >  2:456763/6‑140 (MQ=0)
                                                                                                                ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  2:121580/136‑1 (MQ=2)
                                                                                                                aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                            <  2:563226/136‑1 (MQ=9)
                                                                                                                aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                            <  2:346158/136‑1 (MQ=2)
                                                                                                                 tcgggggTTCTGTTGCAAAGTTGAATTTCTAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  1:241124/136‑1 (MQ=255)
                                                                                                                 taggaGGTTCTGTTGGGAAGTTTAATTTATAATATAATTTTAACAAAAAGGCGTTTTCTGTATGAACTATTTCAGATCTAAACAATTTACCAAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTGAg                           <  2:26950/137‑1 (MQ=11)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGCACTCGAGCGGAGATAAATCATGTAACGGTGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  1:826271/137‑1 (MQ=11)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  1:63044/137‑1 (MQ=9)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  1:400168/137‑1 (MQ=2)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           >  2:666001/5‑141 (MQ=0)
                                                                                                                 atatAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:144465/141‑1 (MQ=39)
                                                                                                                 atatAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAATGTCGCCGCCGGCTACTATCTAAGATATGCATTGAg                           <  1:615414/141‑1 (MQ=255)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:402895/137‑1 (MQ=9)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           >  2:564788/5‑141 (MQ=2)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           >  2:467216/5‑141 (MQ=2)
                                                                                                                 aagatggTACTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:708719/136‑1 (MQ=255)
                                                                                                                  atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAACGAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  2:63260/138‑1 (MQ=11)
                                                                                                                  atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  2:166880/138‑1 (MQ=255)
                                                                                                                  aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCCTTGAgc                          >  2:643842/4‑140 (MQ=255)
                                                                                                                  aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  1:775667/138‑2 (MQ=0)
                                                                                                                   ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                         <  2:627297/139‑3 (MQ=9)
                                                                                                                    taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                        >  1:128595/1‑141 (MQ=37)
                                                                                                                     aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGGGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:268812/1‑141 (MQ=0)
                                                                                                                     aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:455949/1‑141 (MQ=11)
                                                                                                                     aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                       >  1:762646/1‑141 (MQ=0)
                                                                                                                     aGGTTCTGTTGCAAAGTCGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTATCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       <  1:31220/141‑1 (MQ=2)
                                                                                                                       gTTCTGTTGCAAAGTTGAATGTATAGTATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCg                     >  1:543065/1‑141 (MQ=12)
                                                                                                                        ttttGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                    <  1:87627/138‑1 (MQ=2)
                                                                                                                        ttCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                    >  2:706590/1‑141 (MQ=34)
                                                                                                                        ttCTGTTGCAAAGTTGAAGTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                    <  2:459832/141‑1 (MQ=255)
                                                                                                                               gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTGAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGATGGCTACTATCTAAGATATGCATTGAGTTATCATGatctat             >  2:669444/1‑141 (MQ=255)
                                                                                                                                cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTACGATATGCATTGCGTTATCGTGATATATc            >  1:495799/1‑141 (MQ=21)
                                                                                                                                cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc            <  2:726553/141‑1 (MQ=35)
                                                                                                                                cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc            <  1:335194/141‑1 (MQ=35)
                                                                                                                                 aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt           <  1:490584/141‑1 (MQ=34)
                                                                                                                                  aatttGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg          <  1:368990/138‑1 (MQ=2)
                                                                                                                                  aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg          >  1:288021/1‑141 (MQ=35)
                                                                                                                                    gTTGAATCTATAGTATCATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa        >  1:429732/1‑141 (MQ=25)
                                                                                                                                        aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAatat    >  2:822604/1‑141 (MQ=35)
                                                                                                                                          tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa  <  1:411617/141‑1 (MQ=34)
                                                                                                                                          ccTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa  <  1:473283/140‑1 (MQ=18)
                                                                                                                                           |                                                                                                                                           
GAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76041‑76319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: