BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,501	G→A	5.2%	A17A (GCC→GCT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,501	0	G	A	5.2%	300.6 / 3.4	124	A17A (GCC→GCT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (56/57); new base A (3/4); total (59/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 126 total aligned reads displayed.

TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATA  >  CP000730/36365‑36641

tcCAAATTTGATACAAACTTGGAAATGCTATTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                                                                          >  1:830863/1‑141 (MQ=11)

tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                                                                          >  1:762679/1‑141 (MQ=35)

         gATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATaa                                                                                                                                 >  2:112495/1‑141 (MQ=35)

                    ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTTCAGTGATAACATCCTTGTTa                                                                                                                      <  2:595522/141‑1 (MQ=17)

                    ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCGTAGATAGTAGCCAACGGCTACAGGGATAACATCCTTGTTa                                                                                                                      >  1:71894/1‑141 (MQ=17)

                     gggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaa                                                                                                                     >  1:18646/1‑141 (MQ=32)

                      ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                                                                                    >  1:2874/1‑141 (MQ=32)

                       gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACATTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAt                                                                                                                   >  2:834053/1‑141 (MQ=17)

                           atTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTTCTTCCCGTTGTCTTAATATTTCTGATCTCTTACGATAACAAAAACTACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                                                                                                               <  1:401720/141‑1 (MQ=11)

                             tCTGGAACCCAACGTGAGGCCCTTGAATGATGAACGTTTACTCCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAGTATTTATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTta                                                                                                             <  1:650565/141‑1 (MQ=11)

                                 gAACCCAATGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATGTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTTATAACATCCTTGTTAAATTGTTTATATc                                                                                                         >  1:581478/1‑141 (MQ=255)

                                 gAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                                                                         >  1:255631/1‑141 (MQ=32)

                                 gAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                                                                         >  2:686882/1‑141 (MQ=32)

                                 gAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACATTGATAACATCCTTGTTAAATGGTTTATATc                                                                                                         >  2:284525/1‑141 (MQ=12)

                                   aCCCAAACGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                                                                                       <  1:179927/141‑1 (MQ=17)

                                    cccAACGGTAGACCGTTGAATTCTGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTAGATCTGa                                                                                                      <  2:464436/141‑1 (MQ=12)

                                    cccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGa                                                                                                      >  1:837695/1‑141 (MQ=32)

                                     tcAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATTTTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAGAACATCCTTGTTAAATTGTTTATATTTGaa                                                                                                     <  2:508739/140‑1 (MQ=12)

                                      cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATGGTTTATATCTGaaa                                                                                                    <  1:547741/141‑1 (MQ=17)

                                           gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                                               <  1:786618/141‑1 (MQ=32)

                                           gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                                               >  2:645567/1‑141 (MQ=32)

                                           gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                                               >  1:470750/1‑141 (MQ=32)

                                           gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                                               <  2:762679/141‑1 (MQ=32)

                                           gTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtt                                                                                               >  1:470960/1‑141 (MQ=32)

                                                   gtGAATGATGAACGGTTAAACCCAGTTCTCTTAAAATTACCGGTATATCACGATCGTTCAATGTCGATCTGGGATATTAGACGTGGGCCACAGGTAGAATAGCCTTGGTATATTGGTTATATCTGAAATAGTTCATACaga                                                                                       <  2:198023/140‑1 (MQ=2)

                                                    tGAATGATTAACGTTTACACCACGTGCCCTGAAGATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaa                                                                                      <  2:54295/141‑1 (MQ=255)

                                                              aCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACGTAGTGGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCtttt                                                                            >  1:478394/1‑141 (MQ=255)

                                                              aCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCtttt                                                                            >  2:691288/1‑141 (MQ=32)

                                                                gTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                                                                          >  2:425974/1‑141 (MQ=32)

                                                                 tttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGt                                                                         >  2:56874/1‑141 (MQ=32)

                                                                  ttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                                                        >  2:149682/1‑141 (MQ=32)

                                                                  ttACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                                                        <  2:484022/141‑1 (MQ=32)

                                                                    tcaccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaa                                                                      <  2:349532/140‑1 (MQ=17)

                                                                     caccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaaa                                                                     >  1:211831/1‑141 (MQ=32)

                                                                          cGTTCCCTTAATATTTCAGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATtat                                                                >  2:765449/1‑141 (MQ=17)

                                                                               ccTTAATAGTGCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtat                                                           <  1:266046/141‑1 (MQ=17)

                                                                                 ttAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaa                                                         >  1:30189/1‑141 (MQ=33)

                                                                                  tAATATTTTATATAAATCACGATAGCTCAATGAATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATaaa                                                        <  1:412/141‑1 (MQ=11)

                                                                                  tAATATTTCAGATATAGCACGATAACTCAATGTATATCTTAGATAATAGCCAACTGCTACAGTTATAATATCCTTGTTAAATTGTTTATATCTCAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                        <  2:247909/141‑1 (MQ=11)

                                                                                      atgtCAGCTATATCACGACAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                    <  1:108250/138‑1 (MQ=255)

                                                                                      atTTCCGATATATCACGATAACTCCATGTACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                    <  1:358286/141‑1 (MQ=255)

                                                                                      atTTCAGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                    <  1:17475/141‑1 (MQ=25)

                                                                                         ttaGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACt                                                 <  1:27951/139‑1 (MQ=25)

                                                                                           aGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACttt                                               >  1:102941/1‑141 (MQ=35)

                                                                                              tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                            >  2:333053/1‑141 (MQ=34)

                                                                                              taaatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                            <  2:699634/138‑1 (MQ=21)

                                                                                                    aCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACACAAGACTCCTTTTTATTAAAATTGTACTATAAATTAACCTTGGGACCAGaa                                      >  1:195328/1‑141 (MQ=11)

                                                                                                     cGATAACTCAATGTATATCTTAGATCGTACCTATCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCACCAGAAc                                     >  1:404794/1‑141 (MQ=11)

                                                                                                      gagaaCTCAATGCATATATTAGATAGTAGCCAACAGCGAAAGTGATAACATCCTTGTTAAATTGTTAATAACTGAAATAGTTCATACAGAAGACCCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcc                                    <  1:535071/138‑1 (MQ=11)

                                                                                                        tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                  >  2:252535/4‑141 (MQ=2)

                                                                                                        tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                                  <  1:143588/141‑1 (MQ=37)

                                                                                                          gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTAAAATTATATTATAAATACAACTTTGCAACAGAACCGtat                                >  2:824909/2‑141 (MQ=255)

                                                                                                          gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                <  2:528203/140‑1 (MQ=16)

                                                                                                          gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTACTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                >  1:662117/2‑141 (MQ=12)

                                                                                                          gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAGCCTGGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                >  1:795425/2‑141 (MQ=255)

                                                                                                           cTCAATGTATATCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatt                               <  1:168676/141‑1 (MQ=38)

                                                                                                           cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                               >  1:86602/1‑141 (MQ=12)

                                                                                                            tCAATGCATATCTTAGATAGTAGCCAACCGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                              <  1:10620/141‑2 (MQ=255)

                                                                                                            tCAATGCATATCTTAGATAATAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                              >  1:469163/1‑140 (MQ=255)

                                                                                                             cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTAATTTAAAGTATATTATAAATTCACCTTTGCAACAGAACCGtatttt                             >  1:567633/1‑139 (MQ=11)

                                                                                                              aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttta                            <  1:344343/141‑4 (MQ=255)

                                                                                                              aaTGAATATCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaact                            <  2:345749/141‑9 (MQ=11)

                                                                                                               atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACATAACCGtattttat                           >  1:125766/5‑137 (MQ=255)

                                                                                                               aTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATgg                           <  1:766548/141‑1 (MQ=255)

                                                                                                                tgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                          <  1:259739/138‑6 (MQ=255)

                                                                                                                tgcatatCTTAGATAGTAAGCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                          <  1:85758/138‑6 (MQ=11)

                                                                                                                tgaatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGCCTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgt                          <  2:664464/138‑11 (MQ=11)

                                                                                                                 gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                         <  1:276641/139‑7 (MQ=255)

                                                                                                                 gTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGaa                         <  1:92717/141‑1 (MQ=255)

                                                                                                                  catatatTAGATAGTAGCCAACAGCTACAGTGATAACAAAATTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTACTTTTTGTTAAAATTATACTATGAATTCAACTTTACAACAGAAcctcttttactata                        >  2:17777/2‑129 (MQ=11)

                                                                                                                  catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                        >  1:255493/2‑129 (MQ=11)

                                                                                                                  catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                        <  1:648907/140‑13 (MQ=11)

                                                                                                                  catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                        <  1:307722/140‑13 (MQ=11)

                                                                                                                  catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                        >  1:629694/2‑134 (MQ=255)

                                                                                                                   gtatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                       <  2:103718/140‑14 (MQ=11)

                                                                                                                   atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATCGTTCATACAGAAGACTCCTTTTTGTTAAAATGATACTATAACTTCAACTTTGCAACAGAACCGTATTATGGAATa                       >  1:190792/1‑141 (MQ=255)

                                                                                                                   atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                       >  2:676832/1‑141 (MQ=14)

                                                                                                                   atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                       <  1:791634/141‑14 (MQ=11)

                                                                                                                   atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                       >  1:409768/1‑128 (MQ=11)

                                                                                                                    tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGATcctcctaactgtatta                      <  1:641070/141‑15 (MQ=11)

                                                                                                                    tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtattg                      >  2:796596/1‑127 (MQ=11)

                                                                                                                    tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                      <  2:768772/141‑10 (MQ=255)

                                                                                                                    tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATag                      >  1:311920/1‑141 (MQ=255)

                                                                                                                    tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATGGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGGAcctcctaactgtatta                      >  2:450868/1‑127 (MQ=11)

                                                                                                                    tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctaatttactatctg                      >  2:805930/1‑127 (MQ=11)

                                                                                                                        ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                  <  1:765449/141‑1 (MQ=255)

                                                                                                                         tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                 <  2:265660/141‑15 (MQ=11)

                                                                                                                             tagtagGCAACGGCTACAGTGATAACTTCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgg             <  2:188222/141‑1 (MQ=255)

                                                                                                                             tagtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgg             <  2:404323/141‑1 (MQ=255)

                                                                                                                              agtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGt            >  2:555297/1‑141 (MQ=255)

                                                                                                                                tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa          >  1:309791/1‑141 (MQ=255)

                                                                                                                                tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa          <  2:470750/141‑1 (MQ=255)

                                                                                                                                tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa          <  2:470960/141‑1 (MQ=255)

                                                                                                                                    cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt      <  1:369046/141‑1 (MQ=255)

                                                                                                                                      aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta    <  1:667835/141‑1 (MQ=255)

                                                                                                                                      aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta    <  1:686882/141‑1 (MQ=255)

                                                                                                                                      aCGCCTACAGTGATAACTTCCTTGTTAAATTGTTTATAGCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta    <  1:284525/141‑1 (MQ=255)

                                                                                                                                       cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGCATCGAGATGTTGGTAACATTtat   >  2:186292/1‑141 (MQ=255)

                                                                                                                                        ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata  >  2:152623/1‑141 (MQ=255)

                                                                                                                                        ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTaata  >  2:365174/1‑141 (MQ=255)

TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATA  >  CP000730/36365‑36641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`