Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,343,438 C→T 27.1% intergenic (+114/+250) USA300HOU_2200 → / ← alsD hypothetical protein/acetolactate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,343,4380CT27.1% 127.0 / 50.5 82intergenic (+114/+250)USA300HOU_2200/alsDhypothetical protein/acetolactate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (21/38);  new base T (10/12);  total (32/50)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

CAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTAT  >  CP000730/2343301‑2343576
                                                                                                                                         |                                                                                                                                          
cAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCATGCCCGGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAAt                                                                                                                                         <  1:785040/141‑1 (MQ=255)
cAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAAt                                                                                                                                         <  2:787076/141‑1 (MQ=255)
  gATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAg                                                                                                                                       <  1:424875/141‑1 (MQ=255)
  aaTACAAATTGGAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTACTGACCTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAg                                                                                                                                       <  2:183271/140‑1 (MQ=255)
   aTACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGt                                                                                                                                      <  1:574246/141‑1 (MQ=255)
       aaaTTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGTGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAAt                                                                                                                                  <  2:73393/141‑1 (MQ=255)
        aaTTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGAGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATATTAAAtt                                                                                                                                 >  2:529758/1‑141 (MQ=255)
           tGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTta                                                                                                                              <  2:496030/141‑1 (MQ=255)
             tAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTtata                                                                                                                            <  1:778866/141‑1 (MQ=255)
              aGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTatat                                                                                                                           <  2:44218/141‑1 (MQ=255)
                ttAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGa                                                                                                                         >  1:106965/1‑141 (MQ=255)
                 tAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAg                                                                                                                        >  2:163855/1‑141 (MQ=255)
                  aaaTAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAgt                                                                                                                       <  2:182566/141‑1 (MQ=255)
                   aataataatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGCgtt                                                                                                                      >  2:375506/1‑140 (MQ=255)
                           tttGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACcac                                                                                                              >  1:66494/1‑141 (MQ=255)
                             tGAAAATATTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACaa                                                                                                            <  1:763246/141‑1 (MQ=255)
                               aaaaTAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAtt                                                                                                          >  1:109003/1‑141 (MQ=255)
                               aaaaTAGGTTTATAGGGCAAGCGCTTAAAACTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTAGTGTTGATTGCCTCTCGTAGTAACACATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAtt                                                                                                          <  2:776273/141‑1 (MQ=255)
                                aaaTAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAttt                                                                                                         <  1:45227/141‑1 (MQ=255)
                                 aaTAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                                                                        <  1:351768/141‑1 (MQ=255)
                                  aTAGTTTTATAGGGCAAGCGCTGAAAAATTTGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTtat                                                                                                       <  1:698731/141‑1 (MQ=255)
                                  aTAGTTTTATAGGGCAAGCGCTGAAAAATTGGCCTTTGTCTTTTTAGTTATTGGACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGTCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTtat                                                                                                       <  2:460330/141‑1 (MQ=255)
                                  aTAGTGTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTtat                                                                                                       <  1:204416/141‑1 (MQ=255)
                                      ttttATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATc                                                                                                   <  2:390634/141‑1 (MQ=255)
                                         tataGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTaa                                                                                                >  1:45425/1‑141 (MQ=255)
                                             gggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACAt                                                                                            <  2:245952/141‑1 (MQ=255)
                                              ggCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATa                                                                                           >  2:738662/1‑141 (MQ=255)
                                               gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATaa                                                                                          >  1:721744/1‑141 (MQ=255)
                                               gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATaa                                                                                          >  2:811480/1‑141 (MQ=255)
                                                cAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAAc                                                                                         <  2:747922/141‑1 (MQ=255)
                                                 aatgtcTGGAAAATTTGCTGTTGTTGTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGTTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATTAGTGTGTACCACAATTTATAATCTAAACATAACa                                                                                        <  2:346249/136‑1 (MQ=255)
                                                 aaGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                                                                        <  1:749960/141‑1 (MQ=255)
                                                        taaaaaTTTGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTACTGTTGTCTGCTTCTCGTAGTAACAGATTGGCCTACATAATAGTAAATTTTATATTAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtc                                                                                 <  2:254560/140‑1 (MQ=255)
                                                        gAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtc                                                                                 <  2:67671/141‑1 (MQ=255)
                                                         aaaaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtct                                                                                <  1:453423/141‑1 (MQ=255)
                                                          aaaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtctc                                                                               >  1:95374/1‑141 (MQ=255)
                                                            aaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCaa                                                                             >  2:523487/1‑141 (MQ=255)
                                                               tGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAata                                                                          <  1:464468/141‑1 (MQ=255)
                                                                ggCGGTTGTCTTTTTAGATCTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAatat                                                                         >  2:95004/1‑141 (MQ=255)
                                                                 gCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACCGCactgctctagtctc                                                                        <  2:388745/141‑14 (MQ=255)
                                                                  cGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGTCCTATATAATAGTAATTTTTATATGAGTGTGTACCACGATTTATAATCTATACATACCAGCAAACCTCAAttatat                                                                       >  2:499569/1‑139 (MQ=255)
                                                                     ttGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGAACATCTCAAATATCTGGa                                                                    <  1:203657/141‑1 (MQ=255)
                                                                         cTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCt                                                                >  2:342830/1‑141 (MQ=255)
                                                                           ttttAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCtaa                                                              <  1:34033/141‑1 (MQ=255)
                                                                            tttATTTTTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCtaat                                                             <  1:160858/141‑1 (MQ=255)
                                                                               aGTTATTGAACTTGTAAATTATTGTTGTTTACTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATaaa                                                          <  1:480806/141‑1 (MQ=255)
                                                                                   aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTACACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATccc                                                      <  1:277013/141‑1 (MQ=255)
                                                                                   aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATccc                                                      >  2:276691/1‑141 (MQ=255)
                                                                                   aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATccc                                                      <  1:495372/141‑1 (MQ=255)
                                                                                    ttGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATcccc                                                     <  1:89506/141‑1 (MQ=255)
                                                                                     tGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCt                                                    <  1:125213/141‑1 (MQ=255)
                                                                                     tGAACTTGTAAATTATTGTTGTTAGCTTCTCGTAGTAACAGATTGGCCTATACAATAGCAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATTTGGAACCTAATAGATCCCCt                                                    <  1:527959/141‑1 (MQ=255)
                                                                                        aCTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCac                                                 >  1:765475/1‑141 (MQ=255)
                                                                                          ttGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCacta                                               >  1:313180/1‑141 (MQ=255)
                                                                                             tAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAACTTTATATGAGTGTGTACCACTATTGATTATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTg                                            <  2:546565/141‑1 (MQ=255)
                                                                                               aaTTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGca                                          >  2:305064/1‑140 (MQ=255)
                                                                                               aaTTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCt                                          >  2:288356/1‑141 (MQ=255)
                                                                                               aaTTATTGTTGTTTGCTTCGCGTAGTAACAGTTTGGATGATAGAATAGTAAATTTTATTTGAGTGTGTACCACAATTTATAATCTAAACATAACATCAAAACTCACATATCTGGAAACTAATAAATCCCTGCACTACTGCt                                          >  2:303328/1‑141 (MQ=255)
                                                                                                attatttcggttGGCTGCTCGTAGTAACAGATTGGCCGTTACATTAGGAAATTTATCATGAGTGTGTACCACAATGTAAAGTTTAAACACAACATCAACTCTCAAATATCTGGAACCTAATAAATCCCCTCACTACGGCTa                                         <  2:123961/132‑1 (MQ=255)
                                                                                                 ttattGTTGTTTGCTTCCCGTAGTAACAGATTTTGCGATACAATTGTCATGAATATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTaa                                        <  1:819426/141‑1 (MQ=255)
                                                                                                  tattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAAt                                       <  2:45425/141‑1 (MQ=255)
                                                                                                     tgttgtTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGt                                    <  2:592952/141‑1 (MQ=255)
                                                                                                      gttgttTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTg                                   <  2:431792/141‑1 (MQ=255)
                                                                                                                tctcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCCCACTTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGTACCTAATAAATCCCCCCACTACTGCTAATAGTTAAGGGattta                         >  2:790471/1‑141 (MQ=255)
                                                                                                                tctcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATCACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGattta                         <  2:506047/141‑1 (MQ=255)
                                                                                                                 ctcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttat                        <  2:66494/141‑1 (MQ=255)
                                                                                                                  tcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttatt                       >  1:298112/1‑141 (MQ=255)
                                                                                                                  tcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttatt                       <  2:106965/141‑1 (MQ=255)
                                                                                                                  tcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGAAGCCTAATAAGTCCCCTCACTACTGCTAATAGTGAAGGGatttatt                       >  1:435186/1‑141 (MQ=255)
                                                                                                                  ccGTAGTAATAGATTGGCCTATACAATAGTAAATTTTATATGAGTGGGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttatt                       <  1:375506/140‑1 (MQ=255)
                                                                                                                   cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGatttattt                      >  2:42333/1‑141 (MQ=255)
                                                                                                                   cGTAGTAAAAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATGTATAATCTAAACATAAAAGCAAAGCTCAAAAATCTGTAACCTAATAAATATCCTAACTACTGCTAATATTGAAGGGatttattt                      >  2:322830/1‑141 (MQ=255)
                                                                                                                      agtaACAGATTGGCCTATACAACAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGAtttattttat                   <  1:523732/141‑1 (MQ=255)
                                                                                                                        taACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTttatta                 >  2:659275/1‑141 (MQ=255)
                                                                                                                        taACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTttatta                 >  2:741878/1‑141 (MQ=255)
                                                                                                                        aaaCAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTttatta                 <  2:646604/140‑1 (MQ=255)
                                                                                                                          aCAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACt               >  2:535052/1‑141 (MQ=255)
                                                                                                                                 ggCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGt        <  1:143496/141‑1 (MQ=255)
                                                                                                                                 ggCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGt        <  1:414021/141‑1 (MQ=255)
                                                                                                                                   ccTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTaa      >  2:481395/1‑141 (MQ=255)
                                                                                                                                     tataCAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAAtt    >  2:111750/1‑141 (MQ=255)
                                                                                                                                       taCAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATtat  >  2:666471/1‑141 (MQ=255)
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CAGATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTAT  >  CP000730/2343301‑2343576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: