breseq version 0.32.1 revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 39,378 | C→A | 100% | D586Y (GAT→TAT) | mecA ← | penicillin binding protein 2 prime |
RA | 76,969 | G→A | 100% | intergenic (+1027/‑107) | USA300HOU_0072 → / → USA300HOU_0075 | universal stress protein/lysophospholipase |
RA | 110,651 | C→T | 7.9% | D170D (GAC→GAT) | USA300HOU_0109 → | staphylococcal tandem lipoprotein |
RA | 110,660 | T→A | 14.3% | I173I (ATT→ATA) | USA300HOU_0109 → | staphylococcal tandem lipoprotein |
RA | 111,512 | A→G | 30.8% | S194S (TCA→TCG) | USA300HOU_0110 → | possible staphylococcal tandem lipoprotein |
RA | 111,516 | C→A | 31.3% | Q196K (CAA→AAA) | USA300HOU_0110 → | possible staphylococcal tandem lipoprotein |
RA | 111,560 | T→C | 10.4% | R210R (CGT→CGC) | USA300HOU_0110 → | possible staphylococcal tandem lipoprotein |
RA | 195,286 | T→A | 100% | intergenic (‑97/‑82) | USA300HOU_0183 ← / → USA300HOU_0184 | hypothetical protein/hypothetical protein |
RA | 217,384 | A→G | 7.1% | M344T (ATG→ACG) | USA300HOU_0199 ← | possible LIVCS family branched chain amino acid:cation symporter |
RA | 228,319 | T→C | 6.8% | intergenic (+165/‑523) | USA300HOU_0207 → / → hsdR2 | RpiR family transcriptional regulator/type I site‑specific deoxyribonuclease restriction subunit |
RA | 310,609 | A→G | 4.8% | intergenic (‑54/‑95) | USA300HOU_0275 ← / → USA300HOU_0276 | GntR family transcriptional regulator/PTS family glucose/glucoside (glc) porter component IIABC |
RA | 396,540 | G→A | 100% | A208T (GCA→ACA) | USA300HOU_0364 → | possible lipoprotein |
RA | 495,289 | G→A | 7.7% | Q277* (CAG→TAG) | USA300HOU_0470 ← | hypothetical membrane protein |
RA | 512,643 | A→G | 48.4% | intergenic (+711/‑150) | recR → / → rrs1 | recombination protein RecR/16S ribosomal RNA |
RA | 607,884 | G→A | 100% | R209* (CGA→TGA) | USA300HOU_0550 ← | deoxynucleoside kinase |
RA | 629,681 | C→A | 100% | L84I (CTA→ATA) | USA300HOU_0564 → | possible 3‑hexulose‑6‑phosphate synthase |
RA | 665,392 | T→G | 100% | L79* (TTA→TGA) | USA300HOU_0608 → | possible acetytransferase |
RA | 678,888 | G→T | 11.2% | intergenic (+91/+160) | USA300HOU_0621 → / ← sarA | hydrolase/staphylococcal accessory regulator A |
RA | 707,910 | A→G | 100% | intergenic (‑1/‑121) | USA300HOU_0653 ← / → USA300HOU_0654 | ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator |
RA | 744,511 | G→C | 5.1% | intergenic (+2348/‑578) | USA300HOU_0687 → / → sarX | hypothetical membrane protein/possible staphylococcal accessory protein X |
RA | 826,969 | G→T | 6.3% | A36D (GCT→GAT) | USA300HOU_0775 ← | hypothetical membrane protein |
RA | 1,154,017 | C→A | 8.5% | A181E (GCA→GAA) | USA300HOU_1079 → | MutS family DNA mismatch repair protein |
RA | 1,233,504 | T→A | 100% | V470D (GTT→GAT) | USA300HOU_1157 → | non‑specific serine/threonine protein kinase |
RA | 1,243,307 | A→T | 5.0% | S249C (AGT→TGT) | USA300HOU_1165 → | fatty acid/phospholipid synthesis protein |
RA | 1,274,208 | C→T | 100% | S204L (TCA→TTA) | USA300HOU_1186 → | CodY family transcriptional regulator |
RA | 1,416,458 | A→T | 100% | I216N (ATT→AAT) | USA300HOU_1314 ← | oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein |
RA | 1,440,085 | G→T | 100% | intergenic (‑45/‑891) | cspA ← / → xpaC | cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC |
RA | 1,513,930 | A→T | 100% | L413F (TTA→TTT) | USA300HOU_1379 → | hypothetical membrane protein |
RA | 1,646,189 | G→C | 100% | A155G (GCA→GGA) | USA300HOU_1531 ← | M24B family Xaa‑Pro dipeptidase |
RA | 1,685,149 | T→A | 5.3% | intergenic (‑161/+59) | USA300HOU_1575 ← / ← rpsU | hypothetical membrane protein/ribosomal protein S21 |
RA | 1,744,362 | G→A | 100% | H104Y (CAC→TAC) | apt ← | adenine phosphoribosyltransferase |
RA | 1,761,889 | C→A | 100% | D388Y (GAT→TAT) | folC ← | tetrahydrofolate synthase |
RA | 1,857,867 | C→T | 9.0% | L169L (TTG→TTA) | USA300HOU_1728 ← | hypothetical protein |
RA | 1,909,490:1 | +T | 100% | coding (297/303 nt) | USA300HOU_1771 → | hypothetical membrane protein |
RA | 1,963,768 | C→G | 100% | V365L (GTA→CTA) | hemG ← | protoporphyrinogen oxidase |
RA | 2,010,363 | C→T | 100% | C146Y (TGT→TAT) | USA300HOU_1861 ← | ABC superfamily ATP binding cassette transporter, membrane protein |
RA | 2,028,269 | G→C | 100% | P126A (CCA→GCA) | USA300HOU_1882 ← | hypothetical membrane protein |
RA | 2,077,013 | T→C | 9.8% | Y86C (TAT→TGT) | USA300HOU_1933 ← | hypothetical membrane protein |
RA | 2,171,944 | A→G | 6.8% | N509D (AAT→GAT) | leuA2 → | 2‑isopropylmalate synthase |
RA | 2,211,990 | C→T | 5.9% | V196I (GTC→ATC) | USA300HOU_2087 ← | hypothetical membrane protein |
RA | 2,235,173 | C→G | 8.3% | A185A (GCG→GCC) | tdk ← | thymidine kinase |
RA | 2,279,705 | G→A | 100% | intergenic (+1373/+6659) | mtlD → / ← glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
RA | 2,352,815 | G→A | 100% | A92V (GCA→GTA) | rpsI ← | ribosomal protein S9 |
RA | 2,371,220 | C→T | 17.1% | intergenic (‑4337/+1221) | rpmC ← / ← USA300HOU_2234 | ribosomal protein L29/NCS2 family nucleobase:cation symporter‑2 |
RA | 2,384,414 | T→C | 7.3% | Y217C (TAT→TGT) | fmhB ← | peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB |
RA | 2,454,626 | A→T | 100% | L48L (CTA→CTT) | USA300HOU_2315 → | hypothetical membrane protein |
RA | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | USA300HOU_2392 → | FemAB family peptidoglycan biosynthesis protein |
RA | 2,632,292 | C→A | 15.6% | intergenic (‑83/+599) | fnbB ← / ← fnbA | fibronectin‑binding protein B/fibronectin‑binding protein A |
RA | 2,632,296 | G→A | 14.2% | intergenic (‑87/+595) | fnbB ← / ← fnbA | fibronectin‑binding protein B/fibronectin‑binding protein A |
RA | 2,816,469 | G→C | 15.2% | Y620* (TAC→TAG) | USA300HOU_2654 ← | serine‑threonine rich protein |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP000730 | 512620 = | 73 (1.120) | 54 (1.200) +40 bp |
40/180 | NT | 51.7% | intergenic (+688/‑173) | recR/rrs1 | recombination protein RecR/16S ribosomal RNA |
? | CP000730 | 1998278 = | NA (NA) | intergenic (‑6/+5) | USA300HOU_t0050/rrf4 | tRNA‑Val/5S ribosomal RNA | |||||
* | ? | CP000730 | 748995 = | 82 (1.250) | 3 (0.050) | 3/256 | NT | 3.6% | coding (64/96 nt) | USA300HOU_0695 | hypothetical protein |
? | CP000730 | 749026 = | 80 (1.240) | coding (95/96 nt) | USA300HOU_0695 | hypothetical protein | |||||
* | ? | CP000730 | = 800928 | 68 (1.040) | 6 (0.100) | 5/246 | NT | 7.5% | intergenic (+62/‑289) | hisC1/USA300HOU_0748 | histidinol‑phosphate transaminase/hypothetical protein |
? | CP000730 | 801948 = | 83 (1.340) | intergenic (+189/+435) | USA300HOU_0748/dgkA1 | hypothetical protein/possible diacylglycerol kinase |