Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,660 T→A 14.3% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6600TA14.3% 188.2 / 23.6 70I173I (ATT→ATAUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (25/35);  new base A (3/7);  total (28/42)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

AAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAT  >  CP000730/110526‑110798
                                                                                                                                      |                                                                                                                                          
aaaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAAATATACATACGGAGATATTTCataa                                                                                                                                      >  1:680491/1‑140 (MQ=255)
    tGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATc                                                                                                                                  >  2:373744/1‑141 (MQ=255)
          ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                            >  1:761299/1‑141 (MQ=255)
             ataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                         <  2:286260/141‑1 (MQ=255)
              aaaaaTCATTCCAACAAAAGAAATGAAAGAAGAAAAAATAAAAAACGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                        <  2:257394/140‑1 (MQ=255)
               agaaTCATGCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAACCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTAGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                                       <  2:498549/139‑1 (MQ=255)
                aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                      <  1:616539/141‑1 (MQ=255)
                    cATTCCAACAAAAGAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTa                                                                                                                  <  1:296303/141‑1 (MQ=255)
                       tctaacaaAAGAAATTAAAGATGTACAGATAAAAAAGGAGATCGACCACTTTAATTTTTTTGTTCAATATGGCGACATTAAAACTTGTACAAATTAGACAGACGGACTAATTTCATAAAATCCGGGGGTGCCGAgatgttg                                                                                                               >  2:111221/4‑135 (MQ=9)
                           aaaaaaGAAATTAAGGATGAAAAAATAAAAAAAGAAAATTATTACTTTAATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                           <  2:78692/139‑1 (MQ=17)
                                aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                      <  1:25302/141‑1 (MQ=255)
                                  aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTGATTCGGCTAAATATc                                                                                                    <  1:565749/141‑1 (MQ=255)
                                     ttAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAt                                                                                                 >  2:511181/1‑141 (MQ=255)
                                     atAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAt                                                                                                 <  2:532423/140‑1 (MQ=255)
                                      tAAAGATGAAAAAATAAAAAAAGAAGTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                <  2:14672/141‑1 (MQ=255)
                                      tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAGAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                >  2:564932/1‑141 (MQ=255)
                                       aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                               >  2:478562/1‑141 (MQ=255)
                                         aGATGAAAAAATAAAAAAAGAAATCGAAACATTTAAGTTATTTGTTCAATATGGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTAGGCTAAatctgacttcac                                                                                             >  2:434806/1‑131 (MQ=17)
                                         aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                             <  1:8571/141‑1 (MQ=255)
                                          gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                            >  1:629170/1‑141 (MQ=255)
                                              aaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                        <  1:412669/141‑1 (MQ=255)
                                                aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTCatgat                                                                                      >  2:697782/1‑141 (MQ=255)
                                                 aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                     <  1:187482/141‑1 (MQ=255)
                                                  aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTGTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                    <  1:256716/141‑1 (MQ=255)
                                                   aTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                   <  2:488889/141‑1 (MQ=255)
                                                     aaaaaaaGAAATCGAAAACTCTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                 <  1:9114/141‑1 (MQ=255)
                                                      aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                >  1:60834/1‑141 (MQ=255)
                                                          aaGAAATCGAAATCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                            >  1:536417/1‑141 (MQ=255)
                                                          aaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                            <  1:686057/141‑1 (MQ=255)
                                                             aaaGCGAAAACTGTAAGTTCTTTGTTCAATATGGCGACTTTAAAAAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                         <  1:316119/141‑1 (MQ=255)
                                                                 cggaaaCTTTGAGTTCTTTGTTCAATGTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                     <  2:488030/138‑1 (MQ=255)
                                                                  gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                                    >  1:696018/1‑141 (MQ=255)
                                                                   aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                   <  1:235267/141‑1 (MQ=255)
                                                                           aaGTTCTTTGTTCAATATGGCGACTTTCTAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                           >  1:755421/1‑141 (MQ=255)
                                                                             gTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaaa                                                         >  2:78275/1‑140 (MQ=255)
                                                                             gTTCTTTGTTCAATAAGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                         >  1:564649/1‑141 (MQ=255)
                                                                                  ttGTTCCATATGCTGACGTTAAAAATGTGAAAAATTATAAAGACGGAAGTTTTTCATATAATCCAGAGGTGCCGAGTTATTCTGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTCCGCAAAAgatatg                                                    <  2:266965/141‑1 (MQ=17)
                                                                                  ttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACACAAAAgatatg                                                    >  1:648340/1‑141 (MQ=255)
                                                                                     ttCTATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                                 <  2:340029/141‑1 (MQ=255)
                                                                                      tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgatat                                                <  1:98928/141‑1 (MQ=255)
                                                                                          tatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcg                                            >  1:63935/1‑141 (MQ=255)
                                                                                          tatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcg                                            >  1:263298/1‑141 (MQ=255)
                                                                                                        agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                              <  1:410376/131‑1 (MQ=14)
                                                                                                        aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGATTAAAGCTcc                              <  2:464176/141‑1 (MQ=255)
                                                                                                         aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                             >  1:425181/1‑141 (MQ=255)
                                                                                                          aTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATCTACCGACGAGTAAAGCTCCaa                            <  2:755421/141‑1 (MQ=255)
                                                                                                          aTTTGAAAAATGATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaa                            <  2:426068/141‑1 (MQ=255)
                                                                                                            ttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                          <  1:643646/135‑1 (MQ=255)
                                                                                                              taaaaaTTATAAAGACGGAAATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                        <  2:497928/140‑1 (MQ=255)
                                                                                                               atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                       >  2:482506/4‑141 (MQ=255)
                                                                                                               aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTGGGCTTAATAGCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                       >  1:714796/1‑141 (MQ=255)
                                                                                                               aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATGCCGACGAGTAAAGCTCCAAAGTTa                       <  1:485891/141‑1 (MQ=255)
                                                                                                                cgcatcataAAGAGGGAGATATATCATATAATCCAGAGGTGCCGAGTTATCCCGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                      <  1:41116/135‑1 (MQ=17)
                                                                                                                aaaaTTATAAAGCCGGAGATATTTCATATAATCCAGTGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                      <  2:619572/141‑1 (MQ=255)
                                                                                                                aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                      <  1:373744/141‑1 (MQ=255)
                                                                                                                 gaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAGTTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAattatt                     >  2:609137/2‑141 (MQ=14)
                                                                                                                     tataAAGGCGGACATATTTCATATAATCCAGCGGCGCAGTGTTATCCGGCTGAACGTTTAATAACAAAATATGAGTATAAAATAAGACAATTACGCATAATATAGTATATACCGACGAGTAAAGCTCCAAAGTTATTGTTa                 <  2:178479/141‑1 (MQ=255)
                                                                                                                     tataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTa                 <  2:244336/141‑1 (MQ=255)
                                                                                                                          aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGCTATACCGACGAGTAAAGCTCCAAAGGTATTGTTAAAAgg            >  1:615666/1‑141 (MQ=255)
                                                                                                                          aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAGGTTATTGTTAAAAgg            >  1:226816/1‑141 (MQ=255)
                                                                                                                          aGACGGAGACATTTCATAAAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtaacaaag            <  2:315012/141‑8 (MQ=255)
                                                                                                                           gatggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt           <  1:466816/138‑1 (MQ=255)
                                                                                                                           gatggAAATATATCATATAACCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt           <  1:251978/138‑1 (MQ=255)
                                                                                                                             tggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGCAAAAGATATGATATACAGACGAGTAAAGATCCAAAGTTATGGTTAAAAGGTTc         >  2:407792/2‑141 (MQ=18)
                                                                                                                             cGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtta         >  1:331639/1‑140 (MQ=255)
                                                                                                                                agaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg      >  1:67996/1‑141 (MQ=255)
                                                                                                                                agaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg      <  1:406671/141‑1 (MQ=255)
                                                                                                                                  atatatCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAGCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGa    <  1:17759/141‑1 (MQ=255)
                                                                                                                                  atatTTCATATAATCCAGTGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGa    <  1:301914/141‑1 (MQ=255)
                                                                                                                                  atatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAGGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGa    <  1:86024/141‑1 (MQ=255)
                                                                                                                                    atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  <  1:31023/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                          
AAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAT  >  CP000730/110526‑110798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: