breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
MC JC | 417,368 | Δ5 bp | coding (227‑231/690 nt) | phoB → | response regulator in two‑component regulatory system with PhoR |
RA | 701,378 | +T | coding (217/1221 nt) | nagC ← | N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor |
RA | 704,008 | C→A | P22Q (CCG→CAG) | nagE → | N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components |
RA | 705,596 | C→T | N551N (AAC→AAT) | nagE → | N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components |
RA | 1,157,900 | +A | coding (32/1434 nt) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
RA | 1,726,169 | T→G | F49L (TTT→TTG) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
MC JC | 3,815,858 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
MC JC | 3,909,311 | Δ774 bp | [pstA]–[pstC] | [pstA], [pstC] | |
MC JC | 4,001,644 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,542 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1293040 | 1303172 | 10133 | 33 [0] | [0] 41 | tdk–[oppB] | tdk, insZ, insZ, insZ, adhE, ychE, oppA, [oppB] |
* | * | ÷ | NC_000913 | 4110718 | 4111511 | 794 | 17 [16] | [16] 17 | tpiA | tpiA |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 257908 = | NA (NA) | 33 (0.780) | 30/284 | 0.4 | 100% | intergenic (+9/‑768) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | = 1293039 | 0 (0.000) | intergenic (‑117/‑488) | hns/tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase | |||||
* | ? | NC_000913 | = 258674 | NA (NA) | 39 (0.930) | 35/282 | 0.2 | 100% | intergenic (+775/‑2) | crl/crl | pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers |
? | NC_000913 | 1303173 = | 0 (0.000) | coding (275/921 nt) | oppB | oligopeptide ABC transporter permease | |||||
* | ? | NC_000913 | 1207790 = | 23 (0.550) | 14 (0.380) | 12/252 | 2.3 | 37.6% | coding (290/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | 1209619 = | 26 (0.700) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 23 (0.550) | 27 (0.720) | 24/252 | 0.6 | 53.8% | coding (305/630 nt) | stfP | e14 prophage, uncharacterized protein |
? | NC_000913 | = 1209602 | 26 (0.700) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1397238 | NA (NA) | 44 (1.050) | 34/284 | 0.2 | 95.7% | intergenic (+146/‑127) | insH1/ynaJ | IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein |
? | NC_000913 | 1755755 = | 2 (0.050) | coding (58/1413 nt) | pykF | pyruvate kinase I | |||||
* | ? | NC_000913 | = 1755758 | 2 (0.050) | 29 (0.690) | 23/284 | 0.9 | 93.5% | coding (61/1413 nt) | pykF | pyruvate kinase I |
? | NC_000913 | = 2102943 | NA (NA) | intergenic (‑32/+1) | insH1/wbbL | IS5 transposase and trans‑activator/pseudogene, lipopolysaccharide biosynthesis protein | |||||
* | ? | NC_000913 | 1979486 = | 5 (0.120) | 48 (1.140) | 30/284 | 0.4 | 90.6% | intergenic (‑271/‑267) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | 2101749 = | NA (NA) | intergenic (‑5/+146) | wbbL/insH1 | pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator | |||||
* | ? | NC_000913 | = 1979489 | 2 (0.050) | 56 (1.330) | 37/284 | 0.1 | 96.5% | intergenic (‑274/‑264) | insA/uspC | IS1 repressor TnpA/universal stress protein |
? | NC_000913 | = 2290111 | NA (NA) | intergenic (‑32/+1) | insH1/yejO | IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |