| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,726,169 | T→G | F49L (TTT→TTG) | nemR → | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,726,169 | 0 | T | G | 97.6% | 118.7 / NA | 42 | F49L (TTT→TTG) | nemR | transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (20/20); minor base A (1/0); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGA > NC_000913/1726034‑1726305 | cacCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTACCGCACTa > 1:431978/1‑143 (MQ=255) ccGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTaaa < 1:596203/143‑1 (MQ=255) aaCATGATACTCGCGAACATCTCCTGACGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAaagaa < 2:23450/143‑1 (MQ=255) cATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGc < 2:540728/143‑1 (MQ=255) cgcgAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGACGTGCCGACAGGGTCCTTCTATCCCCACTCACGCACTAaagaataaataagcaa > 2:594745/1‑132 (MQ=255) aCATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCCCCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATACCTACTTGCGCCCTAACGAAGCGTTTGGCGTTGCCa > 1:304376/1‑143 (MQ=255) tctcCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCCCTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGc < 2:319361/143‑1 (MQ=255) tcCTGGCGACGGGCGCGCAACTTTGCCTGCCACGTGGACTCACCGGGATGGGGCTAAGCGCATTACGAGAAACCGCCGAAGTGCCGCAAGGGACCCGCTATCACACCTCGACCTCCAAAGCAGCGCTTGGCCTTGCCagccgg > 1:248035/1‑138 (MQ=255) tcCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCtt < 1:526323/143‑1 (MQ=255) cTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGa < 2:219804/143‑1 (MQ=255) tgtcGACGGGCGAGCAACTTTGCCTGCAACGTGGAGTCACCGGGATGGGGGTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTGTATCACTTCTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAg < 1:51254/140‑1 (MQ=255) tGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGGCGAAAGGGTCCTTCTCTCGCTGCTTGCGCTCTAAAGAAGCGTTTGGCCGTGCCATGCTGGAg > 2:363604/1‑143 (MQ=255) tGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACCAGTCGGGAACTAAAGAAGCGTTTGGCGTTGCCATGCTTGAg > 2:134087/1‑143 (MQ=255) ggCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGc < 1:303473/143‑1 (MQ=255) cGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGGCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGt < 2:487782/143‑1 (MQ=255) gggCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTGGGCGCTGCCATGCCTGAGCGTCAtt > 1:533730/1‑143 (MQ=255) cGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTAcg > 1:425494/1‑143 (MQ=255) gttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCatat < 1:402224/142‑1 (MQ=255) ttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTGCGCCGCATATc > 2:409476/1‑143 (MQ=255) gggcttgaatgTGGATTCGCCGGGGTGGGGCTAAGCGAATTACTCAATACCGCGGGAGTGCCGAAAGGGTCCTTCGATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCa < 1:698433/133‑1 (MQ=255) cTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCa > 2:974/1‑143 (MQ=255) tGCAACGTGGATTCACCGGGATGGGGCTAAGCGCATCACCAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCACAGCACCAg > 1:600647/1‑143 (MQ=255) tGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGATCCTTCTATCACTACTTGCGCTGTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCCTTACGCCGCATATCACCAg > 1:196291/1‑143 (MQ=255) tGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGCCCTTCTATAAATGATTGCGCGCTAAAGACGCGTTTGGCTATGCCGCGCTTGGGCGACAGTGAGCCGCATAGGCCCAGCgactga > 1:631261/1‑143 (MQ=255) gATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCCCTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCgactgcct > 2:141731/1‑143 (MQ=255) ttCATCGGTATGGGGCTAAGCGAATTGCTAAAACCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAACAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGActgactga < 1:185735/143‑1 (MQ=255) cACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGt < 2:465954/143‑1 (MQ=255) cACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGt < 2:465832/143‑1 (MQ=255) ggATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgctg < 2:237515/143‑1 (MQ=255) ggATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgctg < 2:670843/143‑1 (MQ=255) ggggCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATCTCACCAGCGACTCACTGCGGTGCTGCagt > 1:53928/1‑141 (MQ=255) cGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGGAg > 2:291071/1‑143 (MQ=255) gAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAgg > 1:170196/1‑143 (MQ=255) gAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTAATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAgg > 2:725605/1‑143 (MQ=255) aaaaCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGCCTGACTGCGTTGCTGCAACCCGGCGAAGTTAACTAcag > 1:110976/1‑141 (MQ=255) aCCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGCAGGTAACTACCGCGa > 2:71624/1‑143 (MQ=255) gCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCg > 2:235004/1‑143 (MQ=255) gTGCCGAAAGGGTCCTTCTATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACt > 2:473924/1‑143 (MQ=255) aGGGTCCTTCCATCACTACTTGCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCttatt < 2:431978/143‑1 (MQ=255) tCCTTCTATCACTACTTGCGCTCTCACGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCa < 2:694588/143‑1 (MQ=255) tCACTACTTGCGCTCGAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAacac < 2:558135/143‑1 (MQ=255) ctactTGCGCCCTACAGAAGCGTTCGGCGTTCCCATGCTTGACCGCCATTCCGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGa < 2:175399/143‑1 (MQ=255) | CACCGAACATGATACTCGCGAACATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGA > NC_000913/1726034‑1726305 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |