New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1397238	NA (NA)	44 (1.050)	34/284	0.2	95.7%	intergenic (+146/‑127)	insH1/ynaJ	IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
	?	NC_000913	1755755 =	2 (0.050)					coding (58/1413 nt)	pykF	pyruvate kinase I

ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGA  >  NC_000913/1397102‑1397377                                                                                                                                         |                                                                                                                                            aTAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaact                                                                                                                                       >  2:473587/1‑143 (MQ=11)   aaCGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaactaa                                                                                                                                     <  2:22526/143‑1 (MQ=32)   aaCGCCTTAAAGGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCACAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaactaa                                                                                                                                     <  2:22353/143‑1 (MQ=17)       ccTTAAATGGCGAAGAAACGGTCTAAATAGGCTGCTTCCAGGCATTTACGGGAGAAAAAATCGGCTCAAAACTGAAGGAATGAAATGACTGAGTCAGCCGAGACGAATTTCCCGGCTTATTCGCACGTTCCCTAACTAATGaa                                                                                                                                 >  1:223507/1‑143 (MQ=11)        cgtgcagagCGAAGATACGGTGGAACGCGGCTGATTTAAGGCATTTACGGGAGAAAAAAGCGGCTCAAACGAGAAGAAATGAAATGACTGAGTCAGGCGAGAAGAATTTCCCCGCTTAGTCGCACCTTCCttagctaaaatgc                                                                                                                                <  1:507973/135‑6 (MQ=1)          tAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGc                                                                                                                              >  1:81817/1‑143 (MQ=37)             aTGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGtt                                                                                                                           >  2:558169/1‑143 (MQ=40)                 gggagacgcGGTCTAAATAGGCTGAGTCAAGGCATGTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGAtt                                                                                                                       <  2:2205/135‑1 (MQ=255)                 cGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCTAGAAGAATTTACCCGATTATACGCAACCTCCCTCACTAATGAATCCGTTGAtt                                                                                                                       >  1:348597/1‑143 (MQ=11)                    agaaACGGTCTAAATAGGCTGATTCCAGGCATTTACGGGAGAAAAAATCGGCTCAAACGTGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTCACTAATCAATGCGTTGATTGtc                                                                                                                    >  2:645564/1‑142 (MQ=255)                                  tAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTAAGCCGAGAAGAATTTCCCAGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTAATTGTAAATCCAGCTAagag                                                                                                      >  1:244332/1‑143 (MQ=255)                                          ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTGACTAATCAATGCGt                                                                                                                            <  1:124101/113‑1 (MQ=255)                                          ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTGACTAATCAATGCGt                                                                                                                            >  2:124101/1‑113 (MQ=255)                                          ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGtt                                                                                              <  2:81817/143‑1 (MQ=255)                                            cAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGtttt                                                                                            <  2:16453/143‑1 (MQ=255)                                                gCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCaga                                                                                        >  2:595185/1‑143 (MQ=255)                                                  aTTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAgaggtgaggcctttagtgc                                                                                      <  1:298335/143‑11 (MQ=255)                                                    ttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGACAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAACAg                                                                                    >  1:400614/1‑143 (MQ=255)                                                        gggAGAAAAAATCGGCTCAAAAAAGCCGAAATGATAGTATTGTGGCAGCCGAAAAGAATATCACCACTTATTCGTACCTTCCCTAACTAAACAATTAAATGATTATAAATACACCTAAGACGTGAGCATGTCAGAGCAAACaa                                                                                >  1:54466/1‑143 (MQ=9)                                                            aaaaaaaTCAGCTGAAGCATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTATTCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGt                                                                            <  2:423107/142‑1 (MQ=255)                                                             aaaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAACTCCAGCTAAGAGGTGAGGTCTTCAGAGCAGACAACgcag                                                                           >  1:664933/1‑140 (MQ=255)                                                              aaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGGGa                                                                          >  2:76679/1‑143 (MQ=255)                                                                aaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGCTTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGGCAACGGTGaaa                                                                        >  1:90273/1‑143 (MQ=255)                                                                 aaTCGGCTCAAATATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCTCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAAt                                                                       <  1:374644/143‑1 (MQ=255)                                                                   tCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTCTAAATCCAGCTAAGAGGCGAGGCTTTCCGAGCAGACAACGGTGAAATGt                                                                     >  1:508043/1‑143 (MQ=255)                                                                        tCAAACAGCAAGAACTGAACGGCCTGAGTCAGCCGAGAAGAAGTTCCCCGCTTATTCGCACCTTCCCTAACGAATCAATGCGTTGATTGTACATCCAGCTAAGAGGTGAGCTTTTCAGAGCAGACAACGGTGAAATGTCATgg                                                                <  1:384980/143‑1 (MQ=25)                                                                         cAAACATGAAGAAAGGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGt                                                               <  1:689669/143‑1 (MQ=255)                                                                             cATGAAGAACTGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAGTCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTg                                                           <  1:240812/143‑1 (MQ=255)                                                                              aTGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTGTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGt                                                          >  1:448113/1‑143 (MQ=255)                                                                                 aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTAc                                                       >  2:91409/1‑143 (MQ=255)                                                                                 aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTAc                                                       >  1:667780/1‑143 (MQ=255)                                                                                    atgtcaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGtt                                                    <  1:625852/138‑1 (MQ=255)                                                                                      atgaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTa                                                  >  1:56693/1‑143 (MQ=255)                                                                                       tgaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAg                                                 <  2:711343/143‑1 (MQ=255)                                                                                       ggaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAg                                                 <  1:443620/142‑1 (MQ=255)                                                                                        gaaatgaCTGAGGCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGCTTGCAAATCGAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAgg                                                <  2:656139/143‑1 (MQ=255)                                                                                          aatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTa                                              >  2:82268/1‑143 (MQ=255)                                                                                           atgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTaa                                             <  2:696253/143‑1 (MQ=255)                                                                                                   gTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAAt                                     <  1:595185/143‑1 (MQ=255)                                                                                                   gTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGGTTAGGTAACAGGAAAt                                     >  1:639230/1‑143 (MQ=255)                                                                                                      aGCCGAGAAGAATTTGTCGGCTTATTCGGAACTTCCCTAACTAAGCAGTGAGTTGATGGGAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTg                                  <  2:184011/143‑1 (MQ=255)                                                                                                       gCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGTCAACGGTGAAATGTCATGGTATTGTTACGTTGAGGTAACAAGAAATTTga                                 >  1:439416/1‑142 (MQ=255)                                                                                                       gCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGt                                 <  2:405969/143‑1 (MQ=255)                                                                                                        ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGGAATGTCATGGTGCTGTGAAGGCTAGGTAACAAGAAATTTGTc                                >  1:624297/1‑143 (MQ=38)                                                                                                        ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTc                                <  1:558169/143‑1 (MQ=255)                                                                                                            gaagaaTTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGca                            >  2:35467/1‑143 (MQ=255)                                                                                                                   ttCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTATGAGGGGAGATTCAAAGAGCAGACAACGGTGTAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCAAAAGGAt                     >  2:420294/1‑143 (MQ=255)                                                                                                                   ttCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATATCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGAt                     <  2:179628/143‑1 (MQ=255)                                                                                                                   ttCCCCGCTTAGTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGTTTTTTAGTTCGGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGAt                     <  2:201069/143‑1 (MQ=255)                                                                                                                      cccGCTTATTCGCACCTTCCCTGACTAATCGATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAc                  <  2:627190/143‑1 (MQ=255)                                                                                                                          cTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAcatca              <  2:667780/143‑1 (MQ=255)                                                                                                                           ttATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAcatcat             >  2:485527/1‑143 (MQ=255)                                                                                                                            tATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTCTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATg            <  2:54114/143‑1 (MQ=255)                                                                                                                             agtCGCGCCTGCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGa           <  1:133294/141‑1 (MQ=255)                                                                                                                               tCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTGTCAGAGCAGACAACGGTGAAAGGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGAtt         >  1:593062/1‑143 (MQ=255)                                                                                                                               ctgCACCTTCCCTAACTAATCGATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGAtt         <  1:76679/141‑1 (MQ=255)                                                                                                                                 gCACCTTCCCTAACTATTTATTTCGTTTATTGTATATCCAGTTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTAt       <  2:181941/143‑1 (MQ=255)                                                                                                                                   aCCTTTCGTATCTTATAAAGGCGTGGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATgg     <  1:358715/143‑1 (MQ=255)                                                                                                                                      ttCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGa  >  2:364677/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                            ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGA  >  NC_000913/1397102‑1397377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.