breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 417,391 G→C A84P (GCC→CCC)  phoB → response regulator in two‑component regulatory system with PhoR
RA 702,066 G→C P229R (CCT→CGT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 1,158,384 G→A W172* (TGG→TGA ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,726,507 Δ1 bp coding (485/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,977,594 +C coding (998/1032 nt) galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
MC JC 3,815,858 Δ82 bp [rph][rph] [rph], [rph]
MC JC 3,909,311 Δ774 bp [pstA][pstC] [pstA], [pstC]
JC 4,001,644 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,583,863 C→T R967H (CGT→CAT)  hsdR ← endonuclease R Type I restriction enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423753–3424232 3424530–3424237 6–778 10 [9] [9] 10 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3762360–3764071 3765192–3764079 9–2833 10 [8] [9] 10 rhsA Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
* * ÷ NC_000913 4110752 4111476 725 10 [9] [8] 10 tpiA triosephosphate isomerase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 153986 =1 (0.030)54 (1.800) 34/284 0.0 98.2% coding (1441/2598 nt) htrE putative outer membrane usher protein
?NC_000913 = 275149 NA (NA)intergenic (‑32/‑176) insH1/mmuP IS5 transposase and trans‑activator/CP4‑6 prophage, putative S‑methylmethionine transporter
* ? NC_000913 = 1539890 (0.000)23 (0.770) 19/284 0.5 100% coding (1438/2598 nt) htrE putative outer membrane usher protein
?NC_000913 273955 = NA (NA)intergenic (+1/+146) ykfC/insH1 CP4‑6 prophage, conserved protein,Phage or Prophage Related/IS5 transposase and trans‑activator
* ? NC_000913 257908 =NA (NA)27 (0.900) 23/284 0.3 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 1207790 =7 (0.230)18 (0.680) 16/252 0.6 76.3% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 5 (0.190)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 12078057 (0.230)18 (0.680) 16/252 0.6 76.3% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 5 (0.190)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =2 (0.070)43 (1.430) 35/284 0.0 95.6% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)41 (1.370) 29/284 0.1 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794893 (0.100)21 (0.700) 20/284 0.5 87.5% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2375822 =1 (0.030)21 (0.720) 18/276 0.6 95.6% coding (139/963 nt) menC O‑succinylbenzoyl‑CoA synthase
?NC_000913 = 2515611 NA (NA)intergenic (+170/+30) insL1/yfeA IS186 transposase/putative diguanylate cyclase
* ? NC_000913 = 23758331 (0.030)31 (1.060) 21/276 0.4 97.0% coding (128/963 nt) menC O‑succinylbenzoyl‑CoA synthase
?NC_000913 2514275 = NA (NA)intergenic (+33/‑54) nupC/insL1 nucleoside (except guanosine) transporter/IS186 transposase