breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 468,144 | Δ1 bp | coding (91/339 nt) | glnK → | nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB |
RA | 683,235 | G→T | intergenic (‑32/+218) | gltI ← / ← insH1 | glutamate, aspartate binding protein, periplasmic; part of GltJKLI ABC transporter/IS5 transposase and trans‑activator |
RA | 2,719,426 | T→C | intergenic (‑321/+2) | kgtP ← / ← rrfG | alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
RA | 2,860,429 | Δ1 bp | coding (482/993 nt) | rpoS ← | RNA polymerase, sigma S (sigma 38) factor |
RA | 2,985,002 | G→T | D126Y (GAT→TAT) | ygeG → | SycD‑like chaperone family TPR‑repeat‑containing protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | CP009273 | 345711 | 346921 | 1211 | 18 [16] | [16] 17 | prpC | prpC |
* | * | ÷ | CP009273 | 748619 | 749966 | 1348 | 18 [16] | [16] 18 | gltA | gltA |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | CP009273 | 360104 = | NA (NA) | 100 (2.310) | 74/292 | 0.0 | NA | intergenic (‑38/+557) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
? | CP009273 | = 360286 | NA (NA) | intergenic (‑220/+375) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor | |||||
* | ? | CP009273 | 1203246 = | 6 (0.140) | 28 (0.730) | 26/260 | 0.6 | 85.7% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | 1205075 = | 4 (0.100) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | = 1203261 | 7 (0.160) | 38 (0.980) | 36/260 | 0.1 | 88.1% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | CP009273 | = 1205058 | 4 (0.100) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | CP009273 | = 1391495 | NA (NA) | 50 (1.150) | 39/292 | 0.2 | 98.0% | intergenic (+146/‑127) | insH1/ynaJ | IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein |
? | CP009273 | = 1972751 | 1 (0.020) | intergenic (‑297/‑483) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein | |||||
* | ? | CP009273 | 1972748 = | 1 (0.020) | 58 (1.350) | 44/290 | 0.1 | 98.3% | intergenic (‑294/‑486) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein |
? | CP009273 | = 2283592 | NA (NA) | noncoding (1/1195 nt) | IS5 | repeat region | |||||
* | ? | CP009273 | 2269338 = | 3 (0.070) | 38 (0.880) | 31/292 | 0.5 | 92.7% | coding (587/1026 nt) | yejE | microcin C transporter YejABEF, permease subunit; ABC family |
? | CP009273 | = 3577555 | NA (NA) | intergenic (+15/‑1) | insB1/yrhA | IS1 transposase B/pseudogene, interrupted by IS1E | |||||
* | ? | CP009273 | = 2269346 | 0 (0.000) | 43 (0.990) | 39/292 | 0.2 | 100% | coding (595/1026 nt) | yejE | microcin C transporter YejABEF, permease subunit; ABC family |
? | CP009273 | 3576788 = | NA (NA) | intergenic (+10/‑55) | yrhA/insA | pseudogene, interrupted by IS1E/IS1 repressor TnpA |