| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 683,235 | G→T | intergenic (‑32/+218) | gltI ← / ← insH1 | glutamate, aspartate binding protein, periplasmic; part of GltJKLI ABC transporter/IS5 transposase and trans‑activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 683,235 | 0 | G | T | 97.0% | 87.7 / NA | 33 | intergenic (‑32/+218) | gltI/insH1 | glutamate, aspartate binding protein, periplasmic; part of GltJKLI ABC transporter/IS5 transposase and trans‑activator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (13/19); minor base C (1/0); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA > CP009273/683093‑683372 | ggCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGa > 2:517148/1‑149 (MQ=255) gCGTTTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCATGTGCCTCTCCGTCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAACTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCa < 1:240045/144‑1 (MQ=255) tggttttGTCCAGAGTAGCGCCCGTCGCCGGGGCGGCGTCATCTGGCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCAt < 1:282214/141‑1 (MQ=255) cgcttttGCCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGCCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCAt < 1:613632/141‑1 (MQ=255) gccgccccggcagcgggCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGttt > 2:738072/15‑149 (MQ=255) cTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGCGAgt > 1:484812/1‑144 (MQ=255) tGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTCATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTtgt < 2:31457/149‑1 (MQ=255) ccGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGtgttg < 1:186629/144‑1 (MQ=255) ggcggcGTCAGCTGCCTGTGTCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAAGTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCt < 2:110297/149‑1 (MQ=255) gcgTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCCTATCCAACTCCTTTATCTTCTGCGTTACGAACTCATGGATTCCCGTTGTGAGTGTTTGTGTTGTTATCGTc < 1:634922/144‑1 (MQ=255) ccTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAAGACTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACCCAAGGATACCCGTTGTGAGAGTTTGTGTTGTTATCGTCTGCACCTTTa > 1:618228/1‑144 (MQ=255) ccgTCCGGCCGAAAGCGCCCGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGtgtt < 2:129156/147‑1 (MQ=255) tgccTGCGGAAAGCGCCACGGCGAGGATTGCTGTGGCAGTTTTACGCAATTGCAAATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAgt < 1:138380/142‑1 (MQ=255) tGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCt > 2:51267/1‑149 (MQ=255) gAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTa < 2:344389/149‑1 (MQ=255) aaGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAgg < 2:289701/149‑1 (MQ=255) ccAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGATTGTGTTGTTATCGTCTGCAAATTTATTGTGCAGTGTTGTGCCTGTTAgg > 1:397806/1‑144 (MQ=255) gCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAgg > 1:295548/1‑144 (MQ=255) cGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAGGGTGCgtc > 2:215998/1‑147 (MQ=255) agAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGaa < 2:403056/148‑1 (MQ=255) ggCAGGTTTACGTAATTGCATATCAAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAAt > 2:734926/1‑149 (MQ=255) cAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAGCTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGGGTTGTGCCTGTTGGGGAAGATCCGAATAAGCGTGGAAAttc > 2:147786/1‑149 (MQ=255) cAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAAttc < 2:626695/149‑1 (MQ=255) gTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAAttcttc > 2:145463/1‑149 (MQ=255) aCGTACTTGCATATCCAACTCCTTTACCTTCCGCTTGAAGTACTCAAACATTTGCGGTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCga < 2:724294/149‑2 (MQ=25) tatCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGCGTGTTTGTGTTGTTCTCGTCTGCCACTATATTGTGCAGTGTGGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAAGTCTTCCCGGCTgcat > 1:353772/1‑141 (MQ=38) atCCAACTCCTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtca < 2:23915/149‑1 (MQ=255) cgccTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTAGCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat < 2:490464/147‑1 (MQ=255) cTTTATCTTCTGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTGTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGGAATGCTTCTCGGCTGGCTCCGtcatttc > 1:208726/1‑144 (MQ=38) tcttttggtttACGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTAGCGTCTGCAACTTTATTGTGCTGTGTTGTGCCCGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc < 1:101507/135‑1 (MQ=25) ctGCGTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt < 2:72199/149‑1 (MQ=255) gCGTTAAGACCTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTTAGTCATTTCATTTCTTCATGTTTGa < 2:374458/149‑1 (MQ=255) gTTAAGAACTCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTAATTTCATATCTTCATGtt > 1:289743/1‑144 (MQ=255) | GGCGATTTTGTCCAGAGTACTGCCCGCTGCCGGGGCGGCGTCATCTGCCTGTGCCAGTCCTGCGGAAAGCGCCAGGGCGAGGATTGCTGTGGCAGGTTTACGTAATTGCATATCCAACTCCTTTATCTTCTGCGTTAAGAACGCATGGATACCCGTTGTGAGTGTTTGTGTTGTTATCGTCTGCAACTTTATTGTGCAGTGTTGTGCCTGTTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA > CP009273/683093‑683372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |