Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,985,002 G→T D126Y (GAT→TAT)  ygeG → SycD‑like chaperone family TPR‑repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,985,0020GT100.0% 163.2 / NA 49D126Y (GAT→TAT) ygeGSycD‑like chaperone family TPR‑repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/27);  total (22/27)

CAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCGATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTG  >  CP009273/2984871‑2985147
                                                                                                                                   |                                                                                                                                                 
cAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACgcaatgc                                                                                                                                       >  1:291794/1‑144 (MQ=255)
  gTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATCACTTCCCGTATGATGACTATTCAGCTATTTATCGTATGGGTCAATGTCAGGTTGGGGCTAAAAATATCTATAACGCAATGCAATGttt                                                                                                                                <  2:482444/149‑1 (MQ=255)
      gTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTAt                                                                                                                            >  2:410704/1‑149 (MQ=255)
              aaaaaGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATcac                                                                                                                         >  1:468336/1‑144 (MQ=255)
                aaaGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACat                                                                                                                       >  1:359759/1‑144 (MQ=255)
                          caacaaGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAAt                                                                                                             <  1:619426/144‑1 (MQ=255)
                          caacaaGCATACGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAAGTGTGa                                                                                                        <  2:3864/149‑1 (MQ=255)
                            acaaGCATATGACGTTTCAAATCTAAGTTACAATTAATTAGTTAAGGGTGACTATAAAATTCTTTAGAGGAGGTGTCTTTGGCAGATTGGGGCGAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATtg                                                                                                           >  1:654216/1‑144 (MQ=255)
                               aGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGgatg                                                                                                   <  2:643320/149‑1 (MQ=255)
                                  atatGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCGATGTTTCTATCACATTATAAACAATTGTGAGga                                                                                                     >  1:179149/1‑144 (MQ=255)
                                       aCCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATa                                                                                                <  1:424655/144‑1 (MQ=255)
                                         cTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAgt                                                                                              >  1:32904/1‑144 (MQ=255)
                                         cTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGGTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCAAATTATTCACAAATGTGAGgatgattgg                                                                                              >  1:524537/1‑141 (MQ=255)
                                           ttACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACCATTGTGAGGATGATAGTGTTAaga                                                                                       <  2:554288/149‑1 (MQ=255)
                                            tACAAACTAAGTTCCAATTACTTCCCGTATGATTACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGtt                                                                                           <  1:388545/144‑1 (MQ=255)
                                            tACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAagag                                                                                      <  2:487508/149‑1 (MQ=255)
                                               aaaCTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTaa                                                                                   >  2:324965/1‑149 (MQ=255)
                                                aaCTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAaga                                                                                       <  1:60020/144‑1 (MQ=255)
                                                     aGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCa                                                                             <  2:324755/149‑1 (MQ=255)
                                                         aCAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCa                                                                         <  2:480608/149‑1 (MQ=255)
                                                          cAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCATATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCa                                                                             <  1:485993/144‑1 (MQ=255)
                                                          cAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGAAAAGCGCa                                                                             <  1:679448/144‑1 (MQ=255)
                                                                   cccGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAAc                                                               >  2:126602/1‑149 (MQ=255)
                                                                    ccGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATAtt                                                                   >  1:612294/1‑144 (MQ=255)
                                                                    ccGTATGATGACTATGCAGGTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAAca                                                              >  2:546165/1‑148 (MQ=255)
                                                                       taggatgaCTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGTAGGCATAGATTCaa                                                                <  1:569779/141‑1 (MQ=255)
                                                                          gatgaCTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTaaa                                                        <  2:628810/149‑1 (MQ=255)
                                                                           atgaCTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAAGATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAAc                                                       <  2:163887/149‑1 (MQ=255)
                                                                            tgaCTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACg                                                      >  2:212234/1‑149 (MQ=255)
                                                                                tATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATaa                                                  >  2:531012/1‑149 (MQ=255)
                                                                                 aTTCATTTATTTATCGTATTGGACAATGTCCGATTGGGGCTAAAAATATCTCTAACGCAATGCAATGTTTTTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAAt                                                 <  2:711046/149‑1 (MQ=255)
                                                                                  ttCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAAtt                                                <  2:18986/149‑1 (MQ=255)
                                                                                  ttCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAACCGAAGACATATATTGAACTCTTTAACGa                                                     >  1:400823/1‑144 (MQ=255)
                                                                                   tCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTc                                               <  2:112671/149‑1 (MQ=255)
                                                                                   tCAGTTATTTAACCTATGGGACAATGTCAGATTGGGGCCAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTc                                               <  2:151047/149‑1 (MQ=255)
                                                                                     aGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCag                                             >  2:624898/1‑149 (MQ=255)
                                                                                              tCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATg                                    <  2:24037/149‑1 (MQ=255)
                                                                                                    gggTCAATGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATgg                                   >  1:314368/1‑144 (MQ=255)
                                                                                                        cAATTTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCAtt                          <  2:184910/149‑1 (MQ=255)
                                                                                                         aaTGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTa                         >  2:111464/1‑149 (MQ=255)
                                                                                                         aaTGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTa                         >  2:111545/1‑149 (MQ=255)
                                                                                                          aTGTCAGATTGGGGCTAAAAATATCTATAACGCAATGTAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAACAGATAATGGCTaaac                             >  1:146826/1‑142 (MQ=255)
                                                                                                           tGTCAGATTGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCa                            >  1:266212/1‑144 (MQ=255)
                                                                                                                 cttGGGGCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCAtt                 <  2:155146/148‑1 (MQ=255)
                                                                                                                    ggggCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCa                   >  1:141458/1‑144 (MQ=255)
                                                                                                                    ggggCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTgg              <  2:196907/149‑1 (MQ=255)
                                                                                                                       gCTAAAAATATCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCAttt                <  1:620474/144‑1 (MQ=255)
                                                                                                                          aaaaaGATCTATCACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGTACTCGTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAAt        <  2:295693/149‑1 (MQ=255)
                                                                                                                                atCTATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTg  <  2:68110/149‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                 
CAGTATGTCAGCCCAAAAAGAAATATCAACAAGCATATGACCTTTACAAACTAAGTTACAATTACTTCCCGTATGATGACTATTCAGTTATTTATCGTATGGGTCAATGTCAGATTGGGGCTAAAAATATCGATAACGCAATGCAATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTG  >  CP009273/2984871‑2985147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑