Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 542,271 IS5 (–) +4 bp 42.9% coding (136‑139/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 269666 =NA (NA)5 (0.280) 5/270 NT 32.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 = 542274 10 (0.570)coding (139/1668 nt) fdrA putative NAD(P)‑binding acyl‑CoA synthetase
* ? CP009273 = 270860NA (NA)10 (0.560) 9/270 NT 49.2% noncoding (320/1221 nt) IS30 repeat region
?CP009273 542271 = 10 (0.570)coding (136/1668 nt) fdrA putative NAD(P)‑binding acyl‑CoA synthetase

AGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCA  >  CP009273/542126‑542416
                                                                                                                                                 |                                                                                                                                                 
aGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAg                                                                                                                                                >  1:102027/1‑149 (MQ=255)
          aTGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATAc                                                                                                                                            >  2:126206/1‑143 (MQ=255)
                     gtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGGTACCACGGGTTTCTGGCCTg                                                                                                                           >  1:113177‑M2/125‑149 (MQ=255)
                     cTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGggaaggtgcgaataa                                                                                                                                 <  2:192042‑M2/143‑16 (MQ=255)
                             aaaaaGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGAc                                                                                                                         >  2:153694/1‑143 (MQ=255)
                                 aGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGggaaggtgcgaataagcggggaaattc                                                                                                                     >  2:85366‑M2/1‑116 (MQ=255)
                                   ggggtTTTCAGGGTTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAAAAATATGGACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAac                                                                                                             <  1:63766/146‑1 (MQ=255)
                                         ttCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAacgccacg                                                                                                       >  1:8048/1‑149 (MQ=255)
                                            ttcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGATACCACAGGTTTCTGTCATGACGATTTTAATAACGCCccgccg                                                                                                    >  1:40888‑M2/102‑149 (MQ=255)
                                                   atgccgaatgtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGATACCACAGGTTTCTGGCATGACGCTTTTAATAACGCCACGCCGa                                                                                                   >  2:36457‑M2/95‑143 (MQ=255)
                                                    gTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtca                                                                                                  <  2:101031‑M2/143‑47 (MQ=255)
                                                              aagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGTACGATAGTTGc                                                                                        <  2:180872‑M2/60‑1 (MQ=255)
                                                                    tttCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGGGGCAATTCGt                                                                            >  1:275273/1‑149 (MQ=255)
                                                                        aCGAAAACTCAGCCAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCg                                                                        <  1:175116/149‑1 (MQ=255)
                                                                                tCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctc                                                                >  1:252561‑M2/1‑69 (MQ=255)
                                                                                tCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAgcggcg                                                                >  1:237582/1‑149 (MQ=255)
                                                                                       aTCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCgg                                                         <  1:143742/149‑1 (MQ=255)
                                                                                         cAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGCTTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCgggg                                                       >  1:26033/1‑149 (MQ=255)
                                                                                                ctccatatgccaaacatgatctcatctcagggtcttgttcgcaccttccTTAGCTACCACAGGTTTCTGGCATGACTATTTTAATCACCCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGCAGCGGCGGATGCGGGGATCgcgc                                                <  1:183527‑M2/100‑1 (MQ=255)
                                                                                                   cagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAgg                                             <  1:116376‑M2/103‑1 (MQ=255)
                                                                                                      atgacaaacaggatctcatatcagggacttgtccgcaccttccTTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGa                                          <  1:126322‑M2/106‑1 (MQ=255)
                                                                                                                     tcatatcagggacttgttcgcaccttccTTAGGTACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAGTTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATgcagc                                 >  2:227079‑M2/29‑143 (MQ=255)
                                                                                                                     gatgGGTACGCCCGCCAATAAAGCGTTATTAGggaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttc                           <  1:303571‑M2/149‑118 (MQ=255)
                                                                                                                     gatgGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGCGTATGCAGCCGCTTg                           >  1:14743/1‑149 (MQ=255)
                                                                                                                      catatcagggacttgttcgcaccttccTTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTGGCGAAGCGGCGGATGCGGGGAGCGCGCGGGCGATTATGCAGCAGCTTGa                          >  1:193107‑M2/28‑149 (MQ=255)
                                                                                                                                    cAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGgcgc                  >  2:279077/1‑143 (MQ=255)
                                                                                                                                          accttccTTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTgg      <  1:189467‑M2/142‑1 (MQ=255)
                                                                                                                                             ttccTTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGgcgc   >  1:115178‑M2/5‑149 (MQ=255)
                                                                                                                                             gTTATTAGATACCACAGGTTTCTGGCATGACGATTTGAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAaacaac         <  2:6909/143‑1 (MQ=255)
                                                                                                                                              ttattaGATACCACAGGTTTCTGGCATGACGATTTTAATAACACCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCa  >  1:312564/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AGGAGTTGTTATGATCCACGCCTTTATTAAAAAAGGGTGTTTTCAGGATTCGGTCAGTTTAATGATTATTTCACGAAAACTCAGCGAATCAGAAAATGTTGATGATGTTTCCGTAATGATGGGTACGCCCGCCAATAAAGCGTTATTAGATACCACAGGTTTCTGGCATGACGATTTTAATAACGCCACGCCGAACGATATTTGCGTGGCAATTCGTAGCGAAGCGGCGGATGCGGGGATCGCGCAGGCGATTATGCAGCAGCTTGAAGAGGCGCTAAAACAACTGGCGCA  >  CP009273/542126‑542416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.