Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 542,271 IS5 (–) +4 bp 42.9% coding (136‑139/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 269666 =NA (NA)5 (0.280) 5/270 NT 32.7% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 = 542274 10 (0.570)coding (139/1668 nt) fdrA putative NAD(P)‑binding acyl‑CoA synthetase
* ? CP009273 = 270860NA (NA)10 (0.560) 9/270 NT 49.2% noncoding (320/1221 nt) IS30 repeat region
?CP009273 542271 = 10 (0.570)coding (136/1668 nt) fdrA putative NAD(P)‑binding acyl‑CoA synthetase

TGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTA  >  CP009273/269538‑269801
                                                                                                                                |                                                                                                                                       
tGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATaa                                                                                                                           >  2:151103/1‑143 (MQ=255)
                     gCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGGCTCa                                                                                                >  1:8787/1‑149 (MQ=255)
                     gCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGACGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGCAATTCTACTGGGCt                                                                                                      >  2:60540/1‑143 (MQ=255)
                                 tCGCAAGCGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                          <  2:63247/143‑1 (MQ=255)
                                                          aTGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGCTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCTTTTATTCATGTTTGAGCCGATTTTTtct                                                           <  1:247566/149‑1 (MQ=255)
                                                            gCGAGTGGGCGCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCAGCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATCTCTTCATGTTTGAGCCGAtttt                                                               <  2:166778/143‑1 (MQ=255)
                                                                                                   cTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                        <  2:138860/143‑1 (MQ=255)
                                                                                                     tCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAAGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTCGACCGATTCTTCGc                >  1:224027/1‑149 (MQ=255)
                                                                                                                   aCAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTa  <  1:317358/149‑1 (MQ=37)
                                                                                                                                |                                                                                                                                       
TGACGGCTTTATCTTTCTGGGGCACAGGCTCATTCGCAAACGCAGTCGTTATGGCGAGATGCGAGTGGTCTCAACGATCCCGCAGGAGAAAGCCAGAAACTTCGCCGCATCGCTGACAGCACTGTTATGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTA  >  CP009273/269538‑269801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.