Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	CP009273	542,271	IS5 (–) +4 bp	42.9%	coding (136‑139/1668 nt)	fdrA →	putative NAD(P)‑binding acyl‑CoA synthetase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	269666 =	NA (NA)	5 (0.280)	5/270	NT	32.7%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	= 542274	10 (0.570)					coding (139/1668 nt)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase
*	?	CP009273	= 270860	NA (NA)	10 (0.560)	9/270	NT	49.2%	noncoding (320/1221 nt)	IS30	repeat region
	?	CP009273	542271 =	10 (0.570)					coding (136/1668 nt)	fdrA	putative NAD(P)‑binding acyl‑CoA synthetase

CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTT  >  CP009273/270713‑271001                                                                                                                                                    |                                                                                                                                              cGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTGGCCCCTTCCg                                                                                                                                              >  1:296432/1‑149 (MQ=11)                             gACGGCGCTGACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCCCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGcc                                                                                                                        <  2:30182/143‑1 (MQ=255)                                   gcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTTTCt                                                                                                            >  1:135824/1‑149 (MQ=255)                                         tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat                                                                                                      >  1:99971/1‑149 (MQ=255)                                                    cTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATc                                                                                                 <  2:255347/143‑1 (MQ=255)                                                      gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCg                                                                                         >  1:272140/1‑149 (MQ=255)                                                        cGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCa                                                                                             <  2:131372/143‑1 (MQ=255)                                                                gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAgcg                                                                               <  1:259844/149‑1 (MQ=255)                                                                        atgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCCCCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAGTATTAATCCCCACCCGCTATTAAGCGcc                                                                             >  2:41658/1‑143 (MQ=255)                                                                         tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGcgcg                                                                      >  1:102039/1‑149 (MQ=255)                                                                             tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGCGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCa                                                                  >  1:15919/1‑149 (MQ=255)                                                                             tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGgcg                                                                        >  2:24662/1‑143 (MQ=255)                                                                                tgatgaACCCTGTTCTATGGCTTCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCt                                                               <  1:141481/149‑1 (MQ=255)                                                                                 gatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTg                                                              >  1:262728/1‑149 (MQ=255)                                                                                        ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTgg                                                       >  1:235480/1‑149 (MQ=255)                                                                                                    cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGAGAACATCTGCTTCTCGTGCAGGGTTGAc                                           >  1:223772/1‑149 (MQ=38)                                                                                                     tCCCGACGACAAACATTAGCTCATAGCAGGGACTTGTTCGCATCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGCTTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGTGTCTGGTGCAggg                                                <  2:178295/143‑1 (MQ=255)                                                                                                        aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTg                                             >  2:45646/1‑143 (MQ=255)                                                                                                         gATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGa                                            <  2:125989/143‑1 (MQ=255)                                                                                                             aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTc                                  >  1:23029/1‑149 (MQ=255)                                                                                                                      ctctccgttCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTCGCCGCCCTTTTGGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCTGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGt                               <  2:87035/135‑1 (MQ=255)                                                                                                                      aTCTCCTATCAGGCTGTAGTTCGCCTCTTCCGGATGCGTTATGAGGGGTCGGCCCTTTTTGCATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGt                               <  2:203087/143‑1 (MQ=255)                                                                                                                                         ttCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACctt      <  1:250940/149‑1 (MQ=255)                                                                                                                                           cGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACaaa          <  2:13836/143‑1 (MQ=255)                                                                                                                                             cACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCttt  <  1:114132/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                              CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTATTAAGCGCCCGGCGCGGGCATCTGCGTCTGGTGCAGGGTTGACTTTGCATTCTGTTAACAAACGCGGTATAACAAACCTTCTTT  >  CP009273/270713‑271001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.