breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 1,293,032 IS1 (–) +8 bp intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,862,760 C→T R142R (CGC→CGT ygbK → FliA‑regulated DUF1537 family protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
MC JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 761494 762730 1237 6 [4] [5] 6 [sucA]–[sucB] [sucA],[sucB]
* * ÷ NC_000913 1882527 1882600 74 8 [4] [5] 6 dmlA D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase
* * ÷ NC_000913 3423762–3424234 3424529–3424239 6–768 7 [4] [4] 6 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 225281NA (NA)9 (0.260) 9/238 2.1 NA noncoding (1511/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 225293 NA (NA)noncoding (1523/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 274620 =NA (NA)6 (0.180) 6/230 2.7 NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 315563 =NA (NA)5 (0.150) 5/228 2.9 NA noncoding (335/1255 nt) IS3 repeat region
?NC_000913 315599 = NA (NA)noncoding (371/1255 nt) IS3 repeat region
* ? NC_000913 756106 =9 (0.250)25 (0.720) 21/240 0.7 82.4% coding (200/1767 nt) sdhA succinate dehydrogenase, flavoprotein subunit
?NC_000913 756221 = 2 (0.060)coding (315/1767 nt) sdhA succinate dehydrogenase, flavoprotein subunit
* ? NC_000913 = 7561099 (0.250)15 (0.430) 14/240 1.3 73.7% coding (203/1767 nt) sdhA succinate dehydrogenase, flavoprotein subunit
?NC_000913 = 756216 2 (0.060)coding (310/1767 nt) sdhA succinate dehydrogenase, flavoprotein subunit
* ? NC_000913 856550 =25 (0.700)8 (0.240) 8/234 2.2 26.0% coding (588/1593 nt) ybiT ABC‑F family putative regulatory ATPase
?NC_000913 856575 = 22 (0.650)coding (613/1593 nt) ybiT ABC‑F family putative regulatory ATPase
* ? NC_000913 1207790 =0 (0.000)28 (0.900) 26/216 0.3 91.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 5 (0.160)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078050 (0.000)26 (0.840) 25/216 0.3 91.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 5 (0.160)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 280538020 (0.560)6 (0.180) 5/230 3.0 22.4% coding (566/1203 nt) proV glycine betaine/proline ABC transporter periplasmic binding protein
?NC_000913 = 2805401 23 (0.700)coding (587/1203 nt) proV glycine betaine/proline ABC transporter periplasmic binding protein