Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	2,977,050	Δ46 bp	coding (452‑497/1032 nt)	galR →	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	2977050	2977095	46	75 [2]	[0] 73	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 2977049	2 (0.020)	67 (0.820)	52/262	0.9	98.6%	coding (451/1032 nt)	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
	?	NC_000913_3_KO_pgi	2977096 =	0 (0.000)					coding (498/1032 nt)	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

GCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGA  >  NC_000913_3_KO_pgi/2976947‑2977241                                                                                                                                                      |                                                                                                                                                  acaaaaagagcgtcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctg                                                                                                                                                   >  2:817685‑M2/150‑150 (MQ=255) acaaaaagagcgtcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctg                                                                                                                                                   >  2:286458‑M2/150‑150 (MQ=255) acaaaaagagcgtcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatcctgacttcgcctcattaatgaaccaaatgcccggtatggtgccgttcaaccgtatcctgcctcgctg                                                                                                                                                   >  4:9940‑M2/150‑150 (MQ=255)   aaaaagagcgtcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaaggctcggtatggtgctgatcaaccgtagcctacatggcaggc                                                                                                                                                 >  4:472615‑M2/148‑150 (MQ=255)        gagcgtcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAAcgc                                                                                                                                            <  2:852296‑M2/8‑1 (MQ=255)            gtcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCa                                                                                                                                        <  2:182231‑M2/12‑1 (MQ=255)             tcaggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCAt                                                                                                                                       >  2:876599‑M2/138‑150 (MQ=255)              caggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCAtt                                                                                                                                      >  1:519716‑M2/137‑150 (MQ=255)               aggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCAttt                                                                                                                                     >  1:606825‑M2/136‑150 (MQ=255)                ggccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTa                                                                                                                                    <  2:235426‑M2/16‑1 (MQ=255)                 gccattgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaacgtatcctgcctggctGGCAACGCGTCATTTaa                                                                                                                                   >  4:153544‑M2/134‑150 (MQ=255)                     ttgagcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCa                                                                                                                               <  1:124993‑M2/21‑1 (MQ=255)                         gcaactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCaa                                                                                                                           >  1:515524‑M2/126‑150 (MQ=255)                           aactgatccgccatcgctgttctgcgttggtcgaccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTACTTCAGCAAgg                                                                                                                         <  4:590728‑M2/27‑1 (MQ=255)                           aactgatccgccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAgg                                                                                                                         <  1:733555‑M2/27‑1 (MQ=255)                             ctgatccgccatcgctgtgctgcggtggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccggatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTc                                                                                                                       <  2:247631‑M2/29‑1 (MQ=255)                                     ccatcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGc                                                                                                               <  2:668560‑M2/37‑1 (MQ=255)                                      catcgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCa                                                                                                              >  2:657934‑M2/113‑150 (MQ=255)                                         ggctgtgttgcgttgctcgtccatgccaaaatgatcccggatgcttatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGAAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                                                                           <  2:303884‑M2/41‑1 (MQ=255)                                         cgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                                                                           <  1:408637‑M2/41‑1 (MQ=255)                                         cgctgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcccgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                                                                           <  2:417177‑M2/41‑1 (MQ=255)                                            tgtgctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGtt                                                                                                        >  4:461571‑M2/107‑150 (MQ=255)                                              tgctgcgttggtcgtccatgccaaaatgaccccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtaccctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTAt                                                                                                      <  4:501116‑M2/46‑1 (MQ=255)                                               gctgcgttggtcgtccatgccaaaatgatcccggatgctgatttagcttcattaatgaaacaaatgcccggtatggtgctgatcaaccgtgtcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                                                                     <  4:468166‑M2/47‑1 (MQ=255)                                                   cgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgtg                                                                                                 <  1:817685‑M2/51‑1 (MQ=255)                                                   cgttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgtg                                                                                                 <  1:286458‑M2/51‑1 (MQ=255)                                                     ttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAg                                                                                                                              <  3:204157‑M2/22‑1 (MQ=255)                                                     ttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAg                                                                                                                              >  4:204157‑M2/98‑119 (MQ=255)                                                     ttggtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGct                                                                                               <  1:277164‑M2/53‑1 (MQ=255)                                                        gtcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTa                                                                                            >  1:362687‑M2/95‑150 (MQ=255)                                                         tcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTaa                                                                                           >  3:174507‑M2/94‑150 (MQ=255)                                                         tcgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTaa                                                                                           >  3:252131‑M2/94‑150 (MQ=255)                                                          cgtccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaaggcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                                                                          <  3:376112‑M2/58‑1 (MQ=255)                                                            tccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcgcggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATGTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCa                                                                                        >  4:348496‑M2/91‑150 (MQ=255)                                                            tccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCa                                                                                        >  3:537174‑M2/91‑150 (MQ=255)                                                             ccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCAc                                                                                       >  2:27161‑M2/90‑150 (MQ=255)                                                             ccatgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCAc                                                                                       >  4:408462‑M2/90‑150 (MQ=255)                                                              catgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCAct                                                                                      >  1:588720‑M2/89‑150 (MQ=255)                                                               atgccaaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCActc                                                                                     <  4:424928‑M2/63‑1 (MQ=255)                                                                    aaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTAttt                                                                                >  1:393868‑M2/83‑150 (MQ=255)                                                                    aaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTAttt                                                                                >  1:722076‑M2/83‑150 (MQ=255)                                                                    aaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTAttt                                                                                <  4:415806‑M2/68‑1 (MQ=255)                                                                    aaaatgatcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTAttt                                                                                <  3:579947‑M2/68‑1 (MQ=255)                                                                          atcccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACg                                                                          >  2:43516‑M2/77‑150 (MQ=255)                                                                             ccggatgctgatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCg                                                                       >  4:23553‑M2/74‑150 (MQ=255)                                                                                      gatttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTc                                                              <  1:927119‑M2/86‑1 (MQ=255)                                                                                       ttttagccgcattaatgaaacaaatgcccggtatggtgctgatcaaccgtttcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                                                             <  3:472615‑M2/87‑1 (MQ=255)                                                                                       atttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                                                             <  3:153544‑M2/87‑1 (MQ=255)                                                                                       atttagcctcattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                                                             >  2:918064‑M2/64‑150 (MQ=255)                                                                                                cattaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCGTACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTa                                                    >  4:249321‑M2/55‑150 (MQ=255)                                                                                                  ttaatgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTAtt                                                  >  3:440368‑M2/53‑150 (MQ=255)                                                                                                      tgaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGa                                              <  2:606825‑M2/102‑1 (MQ=255)                                                                                                       gaaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGAt                                             <  2:588720‑M2/103‑1 (MQ=255)                                                                                                        aaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATg                                            >  1:25860‑M2/47‑150 (MQ=255)                                                                                                        aaacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATg                                            >  2:197117‑M2/47‑150 (MQ=255)                                                                                                         aacaaatgcccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGACCGTCTGCAAGGGTATTACGATGc                                           >  3:492319‑M2/46‑150 (MQ=255)                                                                                                                  ccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGa                                  >  2:640257‑M2/37‑150 (MQ=255)                                                                                                                  ccggtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGa                                  <  2:505792‑M2/114‑1 (MQ=255)                                                                                                                     gtatggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAg                               >  2:695347‑M2/34‑150 (MQ=255)                                                                                                                       atggtgctgagcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTg                             <  3:178994‑M2/119‑1 (MQ=255)                                                                                                                         ggtgctgatcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGt                           <  1:657934‑M2/121‑1 (MQ=255)                                                                                                                                 tcaaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGc                   <  2:722076‑M2/129‑1 (MQ=255)                                                                                                                                   aaccgtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCa                 >  4:557819‑M2/20‑150 (MQ=255)                                                                                                                                       gtatcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGa             <  4:199884‑M2/135‑1 (MQ=255)                                                                                                                                         atcctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGAcc           <  2:354757‑M2/137‑1 (MQ=255)                                                                                                                                           cctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGt                           <  3:338007‑M2/121‑1 (MQ=255)                                                                                                                                           cctgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGt                           >  4:338007‑M2/12‑132 (MQ=255)                                                                                                                                             tgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTAt                                                                                  >  3:257958‑M2/10‑75 (MQ=255)                                                                                                                                             tgcctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTAt                                                                                  <  4:257958‑M2/66‑1 (MQ=255)                                                                                                                                                ctggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGt    <  1:372587‑M2/144‑1 (MQ=255)                                                                                                                                                  ggctGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCTGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGa  <  1:561291‑M2/146‑1 (MQ=255)                                                                                                                                                     tGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGc                   <  1:655500‑M2/129‑1 (MQ=255)                                                                                                                                                     tGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGc                   >  2:655500‑M2/2‑130 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGATGCCCTTGCTGAAAGTGGTATTGCGGCCAATGACCGGCTGGTGA  >  NC_000913_3_KO_pgi/2976947‑2977241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑