Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	2,977,050	Δ46 bp	coding (452‑497/1032 nt)	galR →	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	2977050	2977095	46	75 [2]	[0] 73	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 2977049	2 (0.020)	67 (0.820)	52/262	0.9	98.6%	coding (451/1032 nt)	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
	?	NC_000913_3_KO_pgi	2977096 =	0 (0.000)					coding (498/1032 nt)	galR	galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor

ACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTAC  >  NC_000913_3_KO_pgi/2976901‑2977195                                                                                                                                                     |                                                                                                                                                   aCAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTg                                                                                                                                                   >  2:817685‑M1/1‑149 (MQ=255) aCAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTg                                                                                                                                                   >  2:286458‑M1/1‑149 (MQ=255) aCAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATCCTGACTTCGCCTCATTAATGAACCAAATGCCCGGTATGGTGCCGTTCAACCGTATCCTGCCTCGCTg                                                                                                                                                   >  4:9940‑M1/1‑149 (MQ=255)   aaaaaGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAAGGCTCGGTATGGTGCTGATCAACCGTAGCCTACATGGCAggc                                                                                                                                                 >  4:472615‑M1/1‑147 (MQ=255)        gagCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgc                                                                                                                                            <  2:852296‑M1/150‑9 (MQ=255)            gTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtca                                                                                                                                        <  2:182231‑M1/150‑13 (MQ=255)             tCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcat                                                                                                                                       >  2:876599‑M1/1‑137 (MQ=255)              cAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatt                                                                                                                                      >  1:519716‑M1/1‑136 (MQ=255)               aGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcattt                                                                                                                                     >  1:606825‑M1/1‑135 (MQ=255)                ggCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcattta                                                                                                                                    <  2:235426‑M1/150‑17 (MQ=255)                 gCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAAACGTATCCTGCCTGGCTggcaacgcgtcatttaa                                                                                                                                   >  4:153544‑M1/1‑133 (MQ=255)                     ttGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattca                                                                                                                               <  1:124993‑M1/150‑22 (MQ=255)                         gCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaa                                                                                                                           >  1:515524‑M1/1‑125 (MQ=255)                           aaCTGATCCGCCATCGCTGTTCTGCGTTGGTCGACCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttacttcagcaagg                                                                                                                         <  4:590728‑M1/150‑28 (MQ=255)                           aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaagg                                                                                                                         <  1:733555‑M1/150‑28 (MQ=255)                             cTGATCCGCCATCGCTGTGCTGCGGTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGGATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtc                                                                                                                       <  2:247631‑M1/150‑30 (MQ=255)                                     ccATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgc                                                                                                               <  2:668560‑M1/150‑38 (MQ=255)                                      cATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgca                                                                                                              >  2:657934‑M1/1‑112 (MQ=255)                                       aTCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGtt                                                                                                                                        >  2:788631/1‑123 (MQ=255)                                       aTCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGtt                                                                                                                                        <  1:788631/123‑1 (MQ=255)                                         ggcTGTGTTGCGTTGCTCGTCCATGCCAAAATGATCCCGGATGCTTATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggaaacgcgtcatttaattcagcaaggtcatacccgcattg                                                                                                           <  2:303884‑M1/149‑42 (MQ=255)                                         cGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattg                                                                                                           <  1:408637‑M1/150‑42 (MQ=255)                                         cGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCCGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattg                                                                                                           <  2:417177‑M1/150‑42 (MQ=255)                                            tgtgCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggtt                                                                                                        >  4:461571‑M1/1‑106 (MQ=255)                                              tgctgcGTTGGTCGTCCATGCCAAAATGACCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTACCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttat                                                                                                      <  4:501116‑M1/150‑47 (MQ=255)                                               gctgcGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCTTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTGTCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatc                                                                                                     <  4:468166‑M1/150‑48 (MQ=255)                                                   cGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtg                                                                                                 <  1:817685‑M1/150‑52 (MQ=255)                                                   cGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtg                                                                                                 <  1:286458‑M1/150‑52 (MQ=255)                                                     ttGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgct                                                                                               <  1:277164‑M1/150‑54 (MQ=255)                                                     ttGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcag                                                                                                                              >  4:204157‑M1/1‑97 (MQ=255)                                                     ttGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcag                                                                                                                              <  3:204157‑M1/119‑23 (MQ=255)                                                        gtcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctcta                                                                                            >  1:362687‑M1/1‑94 (MQ=255)                                                         tcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaa                                                                                           >  3:252131‑M1/1‑93 (MQ=255)                                                         tcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaa                                                                                           >  3:174507‑M1/1‑93 (MQ=255)                                                          cgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAAGGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaac                                                                                          <  3:376112‑M1/150‑59 (MQ=255)                                                            tcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCGCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatgtaattcagcaaggtcatacccgcattggttatctgtgctctaacca                                                                                        >  4:348496‑M1/1‑90 (MQ=255)                                                            tcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaacca                                                                                        >  3:537174‑M1/1‑90 (MQ=255)                                                             ccATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccac                                                                                       >  2:27161‑M1/1‑89 (MQ=255)                                                             ccATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccac                                                                                       >  4:408462‑M1/1‑89 (MQ=255)                                                              cATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccact                                                                                      >  1:588720‑M1/1‑88 (MQ=255)                                                               aTGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactc                                                                                     <  4:424928‑M1/150‑64 (MQ=255)                                                                    aaaaTGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctattt                                                                                >  1:393868‑M1/1‑82 (MQ=255)                                                                    aaaaTGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctattt                                                                                >  1:722076‑M1/1‑82 (MQ=255)                                                                    aaaaTGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctattt                                                                                <  3:579947‑M1/150‑69 (MQ=255)                                                                    aaaaTGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctattt                                                                                <  4:415806‑M1/150‑69 (MQ=255)                                                                          aTCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacg                                                                          >  2:43516‑M1/1‑76 (MQ=255)                                                                             ccGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccg                                                                       >  4:23553‑M1/1‑73 (MQ=255)                                                                                      gATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtc                                                              <  1:927119‑M1/150‑87 (MQ=255)                                                                                       ttttaGCCGCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTTTCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtct                                                             <  3:472615‑M1/149‑88 (MQ=255)                                                                                       aTTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtct                                                             <  3:153544‑M1/150‑88 (MQ=255)                                                                                       aTTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtct                                                             >  2:918064‑M1/1‑63 (MQ=255)                                                                                                cATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcgtacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggta                                                    >  4:249321‑M1/1‑54 (MQ=255)                                                                                                  ttAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtatt                                                  >  3:440368‑M1/1‑52 (MQ=255)                                                                                                      tGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacga                                              <  2:606825‑M1/150‑103 (MQ=255)                                                                                                       gAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgat                                             <  2:588720‑M1/150‑104 (MQ=255)                                                                                                        aaaCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatg                                            >  1:25860‑M1/1‑46 (MQ=255)                                                                                                        aaaCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatg                                            >  2:197117‑M1/1‑46 (MQ=255)                                                                                                         aaCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagaccgtctgcaagggtattacgatgc                                           >  3:492319‑M1/1‑45 (MQ=255)                                                                                                                  ccGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctga                                  >  2:640257‑M1/1‑36 (MQ=255)                                                                                                                  ccGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctga                                  <  2:505792‑M1/150‑115 (MQ=255)                                                                                                                     gTATGGTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaag                               >  2:695347‑M1/1‑33 (MQ=255)                                                                                                                       aTGGTGCTGAGCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtg                             <  3:178994‑M1/150‑120 (MQ=255)                                                                                                                         ggTGCTGATCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggt                           <  1:657934‑M1/150‑122 (MQ=255)                                                                                                                                 tCAACCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggc                   <  2:722076‑M1/150‑130 (MQ=255)                                                                                                                                   aaCCGTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggcca                 >  4:557819‑M1/1‑19 (MQ=255)                                                                                                                                       gTATCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggccaatga             <  4:199884‑M1/150‑136 (MQ=255)                                                                                                                                         aTCCTGCCTGGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggccaatgacc           <  2:354757‑M1/150‑138 (MQ=255)                                                                                                                                           cctgcctgGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggt                           <  3:338007‑M1/132‑122 (MQ=255)                                                                                                                                           cctgcctgGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggt                           >  4:338007‑M1/1‑11 (MQ=255)                                                                                                                                             tgcctgGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctat                                                                                  >  3:257958‑M1/1‑9 (MQ=255)                                                                                                                                             tgcctgGCTggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctat                                                                                  <  4:257958‑M1/75‑67 (MQ=255)                                                                                                                                                ctggctggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggccaatgaccggctggt    <  1:372587‑M1/150‑145 (MQ=255)                                                                                                                                                  ggctggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgctgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggccaatgaccggctggtga  <  1:561291‑M1/150‑147 (MQ=255)                                                                                                                                                     tggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggc                   <  1:655500‑M1/130‑130 (MQ=255)                                                                                                                                                     tggcaacgcgtcatttaattcagcaaggtcatacccgcattggttatctgtgctctaaccactctatttctgacgccgaagatcgtctgcaagggtattacgatgcccttgctgaaagtggtattgcggc                   >  2:655500‑M1/1‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   ACAAAAAGAGCGTCAGGCCATTGAGCAACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTAC  >  NC_000913_3_KO_pgi/2976901‑2977195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑