New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 3046145221 (1.100)18 (0.140)
+TTAGTCAGATGACAAAGTACAAAAGTGCTCAGACA		 9/126 15.0 12.6% intergenic (‑244/+23) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
?NC_000913_3_KO_pgi = 3504895 170 (0.840)intergenic (+112/+40) frlR/yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 173 total aligned reads displayed.

TAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGC  >  NC_000913_3_KO_pgi/3504796‑3504990
                                                                                                   |                                                                                               
tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                 <  1:1426180/100‑1 (MQ=255)
tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                 <  1:4917388/100‑1 (MQ=255)
   gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGt                                                                                              >  1:2752724/1‑100 (MQ=255)
      gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                             >  2:879399/1‑98 (MQ=255)
      gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                             >  2:4511328/1‑98 (MQ=255)
       cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                           <  2:2252218/99‑1 (MQ=255)
       cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                          >  1:353270/1‑100 (MQ=255)
       cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                          >  1:1674229/1‑100 (MQ=255)
       cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCGCTCCCCAAAGGGGCGAGGGGACCGt                                                                                              <  1:532550/96‑1 (MQ=255)
        gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                          >  1:4037600/1‑99 (MQ=255)
        gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                          >  1:3075638/1‑99 (MQ=255)
        gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                          >  2:3549750/1‑99 (MQ=255)
        gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAg                                                                                          >  2:3769359/1‑99 (MQ=255)
         ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                        >  1:2752274/1‑100 (MQ=255)
           aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                      >  1:1621645/1‑100 (MQ=255)
            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                      <  2:2635326/99‑1 (MQ=255)
             catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                      >  2:2669637/1‑98 (MQ=255)
             catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGa                                                                                    <  1:2321082/100‑1 (MQ=255)
              atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTACAGTACgg                                                                                     >  2:2922649/1‑98 (MQ=255)
                 gcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAt                                                                                  <  1:3651095/98‑1 (MQ=255)
                  catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                 >  2:2161732/1‑98 (MQ=255)
                   atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                               <  2:138419/99‑1 (MQ=255)
                   atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                               <  2:3081578/99‑1 (MQ=255)
                    tcCGGCGGTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCGCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                               <  2:4723061/98‑1 (MQ=255)
                    tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGCCCGTTCAGTACGGAATTTAg                                                                              >  2:4018417/1‑99 (MQ=255)
                    tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                              <  2:3233077/99‑1 (MQ=255)
                    tcCGGCGATGCTGGCGCGTTGAATTTTACATCCAGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGCAAtt                                                                                 >  2:1982055/1‑96 (MQ=255)
                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                             <  2:4660067/98‑1 (MQ=255)
                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCtt                                                                           >  1:4827990/1‑100 (MQ=255)
                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                            >  2:1144424/1‑99 (MQ=255)
                      cGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTGTCCCCAAGGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                             <  2:4857587/98‑1 (MQ=255)
                       ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCCCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                            >  2:739747/1‑98 (MQ=255)
                           aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                      <  1:3028838/100‑1 (MQ=255)
                              cTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                    <  2:2068982/99‑1 (MQ=255)
                              cTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                   >  1:2950569/1‑100 (MQ=255)
                              cTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                   <  1:87740/100‑1 (MQ=255)
                               tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                    >  2:2465149/1‑98 (MQ=255)
                               tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                   >  2:243770/1‑99 (MQ=255)
                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                    <  2:18463/96‑1 (MQ=255)
                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                   <  2:3704538/97‑1 (MQ=255)
                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGCTCAGTACGGAATTTAGCTTGAc                                                                        >  2:4115284/1‑92 (MQ=255)
                                   gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                               <  2:4548691/99‑1 (MQ=255)
                                   gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                               <  2:1770403/99‑1 (MQ=255)
                                   gcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                               <  2:446361/99‑1 (MQ=255)
                                     gTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAg                                                            >  1:4503130/1‑100 (MQ=255)
                                      ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAg                                                            <  1:510805/99‑1 (MQ=255)
                                       tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                          >  1:630357/1‑100 (MQ=255)
                                        gAATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGc                                                           <  2:4161671/98‑1 (MQ=255)
                                         aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAt                                                         <  1:4272117/99‑1 (MQ=255)
                                          aTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGTCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                        <  2:3371854/99‑1 (MQ=255)
                                          aTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                        >  1:2190510/1‑99 (MQ=255)
                                             ttACATCCCGTACGTTCCCGTCACCCGAACCCTCTCCCCGATGGGGCGAGGGGACCGTTCAGTACGGCATTTAGCTTGACGGTGCTGTCCAGCATTaaa                                                     >  1:4579840/1‑99 (MQ=37)
                                                 aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                 <  2:1408946/99‑1 (MQ=255)
                                                  tCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTACAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                               >  1:3905085/1‑100 (MQ=255)
                                                          gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActc                                       <  1:4680074/100‑1 (MQ=255)
                                                          gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActc                                       <  1:2583158/100‑1 (MQ=255)
                                                           ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActc                                       <  2:2554736/99‑1 (MQ=255)
                                                             ccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                    >  1:2412892/1‑100 (MQ=255)
                                                              cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                   >  1:2667565/1‑100 (MQ=255)
                                                              cccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGCACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                    <  1:3908069/99‑1 (MQ=255)
                                                               ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACg                                  >  1:2570275/1‑100 (MQ=255)
                                                               ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACg                                  >  1:4214592/1‑100 (MQ=255)
                                                                  cACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCa                                >  1:1797235/1‑99 (MQ=255)
                                                                    cccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCa                                >  2:3577236/1‑97 (MQ=255)
                                                                     ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaaa                             <  2:2722720/99‑1 (MQ=255)
                                                                       tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcc                          >  1:4609282/1‑100 (MQ=255)
                                                                         aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcca                         <  2:4687240/99‑2 (MQ=255)
                                                                         aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                         <  1:1978112/99‑1 (MQ=255)
                                                                         aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                         >  2:1284454/1‑99 (MQ=255)
                                                                          nccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                         >  1:1944003/2‑98 (MQ=255)
                                                                          cccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                         >  2:809865/1‑98 (MQ=255)
                                                                           ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTGGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                      >  1:2657424/1‑100 (MQ=255)
                                                                           ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                         >  2:928927/1‑97 (MQ=255)
                                                                            ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                      >  1:629944/1‑99 (MQ=255)
                                                                            ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                      >  2:4024125/1‑99 (MQ=255)
                                                                                ccccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCg                 >  1:4773338/1‑100 (MQ=255)
                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                  >  2:1325648/1‑98 (MQ=255)
                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                  <  2:3395851/98‑1 (MQ=255)
                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCg                 >  2:1865737/1‑99 (MQ=255)
                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                >  1:3072800/1‑100 (MQ=255)
                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                >  1:963568/1‑100 (MQ=255)
                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                >  1:949842/1‑100 (MQ=255)
                                                                                  ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc               <  1:1358329/100‑1 (MQ=255)
                                                                                  ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc               <  1:216691/100‑1 (MQ=255)
                                                                                     aaGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTctct            <  1:1529377/100‑1 (MQ=255)
                                                                                     aaGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGATATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtctc             <  2:3576673/99‑1 (MQ=255)
                                                                                      aGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt           >  1:3612710/1‑100 (MQ=255)
                                                                                        gggCGAGGGGCCCGTTCAGTACGGAATTTAGCTTGACGGTGCTGTCCAGCATTAAATACTGGCATAACTCCCACGCAAATTCCGCAGCACCGTCTCtt           >  2:4181965/1‑98 (MQ=255)
                                                                                        gggCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt           <  2:4520241/98‑1 (MQ=255)
                                                                                        gggCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa         >  1:2308359/1‑100 (MQ=255)
                                                                                        gggCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa         >  1:1449351/1‑100 (MQ=255)
                                                                                         ggCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc        <  1:4257048/100‑1 (MQ=255)
                                                                                         ggCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc        >  1:328169/1‑100 (MQ=255)
                                                                                         ggCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc        <  1:836042/100‑1 (MQ=255)
                                                                                          gCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc        <  2:4140488/99‑1 (MQ=255)
                                                                                          gCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc        <  2:1670536/99‑1 (MQ=255)
                                                                                            gAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGc     >  1:3530895/1‑100 (MQ=255)
                                                                                              ggggACAGTTCAGTACGGAACTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCCATACTCTCACGCAAAATCCGCAGCACCg                 >  2:4953591/1‑86 (MQ=255)
                                                                                                ggACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGc  <  2:2254573/99‑1 (MQ=255)
                                                                                                 gACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTCAATACTGGCAATACTCTCCCGCAAAATCCGCAGCACCGTCTCATCACCGCTGc  >  2:3334422/1‑98 (MQ=255)
                                                                                                   |                                                                                               
TAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGC  >  NC_000913_3_KO_pgi/3504796‑3504990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.