| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 3046145 | 221 (1.100) | 18 (0.140) +TTAGTCAGATGACAAAGTACAAAAGTGCTCAGACA |
9/126 | 15.0 | 12.6% | intergenic (‑244/+23) | ygfF/gcvP | putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex |
| ? | NC_000913_3_KO_pgi | = 3504895 | 170 (0.840) | intergenic (+112/+40) | frlR/yhfS | putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein | |||||
| Rejected: Coverage evenness skew score above cutoff. | |||||||||||
| Rejected: Frequency below/above cutoff threshold. | |||||||||||
CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913_3_KO_pgi/3046101‑3046145‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA < NC_000913_3_KO_pgi/3504895‑3504855 ||||||||||||||||||||||||||||||||||| CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGG > 1:2804155/1‑99 CATCTGACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGT > 1:1572778/1‑99 CATCTGACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGT > 1:1636269/1‑99 CTGACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGG > 1:1071847/1‑100 TGACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGG > 1:2903873/1‑100 TGACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGATTAGGGTGAGG > 2:3329982/1‑99 GACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGG > 1:2996915/1‑99 GACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGG > 2:2619155/1‑99 GACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGG > 1:601078/1‑99 GACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGG > 1:2494033/1‑99 GACTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGG > 1:2296109/1‑99 CTAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA > 1:2991002/1‑100 TAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAACGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGGGGGTTAGGGTTAGCGGAA > 2:1277691/1‑99 TAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA > 2:2115734/1‑99 TAAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA > 2:3098417/1‑99 AAAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA > 1:1640884/1‑98 AAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA > 2:1885502/1‑97 AAAAGGCGCCGAAGCGCCTTTTTAGTCAGATGACAAAGTACAAAAGTGCTCAGACAGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA > 2:3395140/1‑97 ||||||||||||||||||||||||||||||||||| CTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913_3_KO_pgi/3046101‑3046145‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAA < NC_000913_3_KO_pgi/3504895‑3504855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |