breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 11,511 C→T 5.4% G92G (GGG→GGA yaaI ← UPF0412 family protein
JC JC 573,890 IS5 (–) +4 bp 92.6% intergenic (+20/‑63) ylcG → / → quuD uncharacterized protein, DLP12 prophage/DLP12 prophage; putative antitermination protein
RA 630,245 A→G 10.9% I118V (ATC→GTC)  cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
RA 657,541 G→T 93.3% intergenic (+40/+14) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
RA 742,578 A→G 5.2% intergenic (‑22/‑249) dtpD ← / → ybgI dipeptide and tripeptide permease D/NIF3 family metal‑binding protein
RA 1,097,158 C→T 12.2% intergenic (+329/+407) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,466,268 A→G 7.9% pseudogene (877/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,435 A→G 5.2% pseudogene (1044/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,604 G→A 8.1% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,708,793 C→G 6.2% A625A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,304,798 T→C 5.2% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,911,534 Δ1 bp 94.0% coding (2118/2235 nt) relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 3,560,455 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,809 +A 6.3% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 4,187,507 A→C 100% N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,190 A→G 16.3% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 22.6% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 24.7% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 21.5% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 12.5% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 10.6% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +C 92.6% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +G 91.7% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,381 +G 5.8% intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,326,088 T→G 7.1% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978429 1979738 1310 64 [61] [62] 65 insB1–insA insB1, insA

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)95 (0.750) 66/536 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
* ? NC_000913 1207790 =61 (0.470)50 (0.410) 40/520 NT 49.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 46 (0.380)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780557 (0.440)43 (0.350) 40/520 NT 46.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 46 (0.380)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)47 (0.370) 36/544 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1497317147 (1.130)3 (0.020) 3/536 NT 2.1% coding (462/1656 nt) opgD OPG biosynthetic periplasmic protein
?NC_000913 = 1497324 143 (1.140)coding (469/1656 nt) opgD OPG biosynthetic periplasmic protein
* ? NC_000913 = 19785020 (0.000)32 (0.250) 31/536 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein