breseq version 0.27.1 revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 88,334 | C→T | P103S (CCA→TCA) | cra → | transcriptional repressor‑activator for carbon metabolism |
RA | 191,723 | C→T | intergenic (+15/‑132) | tsf → / → pyrH | translation elongation factor EF‑Ts/uridylate kinase |
RA | 240,031 | T→C | A53A (GCA→GCG) | yafV ← | putative NAD(P)‑binding C‑N hydrolase family amidase |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 281,708 | T→C | G92G (GGA→GGG) | yagA ← | CP4‑6 prophage; putative DNA‑binding transcriptional regulator |
RA | 300,196 | A→G | F247L (TTC→CTC) | paoC ← | PaoABC aldehyde oxidoreductase, Moco‑containing subunit |
RA | 320,847 | G→A | R207R (CGG→CGA) | rclR → | reactive chlorine species (RCS)‑specific activator of the rcl genes |
RA | 323,369 | G→A | V344V (GTG→GTA) | ykgF → | ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component |
RA | 538,489 | C→T | A286V (GCC→GTC) | ybbW → | putative allantoin transporter |
RA | 647,385 | A→G | V42A (GTG→GCG) | citG ← | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
RA | 864,985 | A→G | Y49H (TAT→CAT) | moeB ← | molybdopterin synthase sulfurylase |
RA | 1,055,493 | G→A | A229V (GCA→GTA) | torS ← | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
RA | 1,121,967 | C→T | G214E (GGA→GAA) | pyrC ← | dihydro‑orotase |
RA | 1,145,744 | C→T | Q269* (CAG→TAG) | rluC → | 23S rRNA pseudouridine(955,2504,2580) synthase |
RA | 1,258,523 | T→C | intergenic (‑10/‑268) | pth ← / → ychH | peptidyl‑tRNA hydrolase/DUF2583 family putative inner membrane protein |
RA | 1,314,127 | A→G | V36V (GTA→GTG) | ompW → | outer membrane protein W |
RA | 1,378,342 | C→T | D153D (GAC→GAT) | ycjT → | putative family 65 glycosyl hydrolase |
RA | 1,529,218 | C→T | pseudogene (1317/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,781,073 | G→A | G225S (GGC→AGC) | ydiR → | putative electron transfer flavoprotein, FAD‑binding subunit |
RA | 1,831,190 | C→T | A265T (GCA→ACA) | astC ← | succinylornithine transaminase, PLP‑dependent |
RA | 1,883,341 | (G)6→7 | coding (154/1446 nt) | yeaV → | putative transporter |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,047,530 | G→A | V865I (GTC→ATC) | yeeJ → | putative adhesin |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,183,628 | (T)7→8 | coding (24/819 nt) | yegX ← | putative family 25 glycosyl hydrolase |
RA | 2,230,157 | G→A | L77L (CTG→TTG) | dusC ← | tRNA‑dihydrouridine synthase C |
RA | 2,296,831 | G→A | A65V (GCG→GTG) | ccmB ← | heme export ABC transporter permease |
RA | 2,515,413 | (C)7→8 | coding (1083/1113 nt) | insL1 → | IS186 transposase |
RA | 2,638,749 | T→C | S179G (AGT→GGT) | yfgM ← | ancillary SecYEG translocon subunit; putative anti‑RcsB factor |
RA | 2,810,153 | Δ1 bp | coding (537/738 nt) | ygaZ → | putative L‑valine exporter, norvaline resistance protein |
RA | 2,856,252 | G→A | A639T (GCC→ACC) | fhlA → | formate hydrogenlyase transcriptional activator |
RA | 3,202,425 | A→G | K179E (AAA→GAA) | cca → | fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase |
RA | 3,255,057 | G→A | L5L (TTG→TTA) | yhaL → | uncharacterized protein |
RA | 3,304,813 | (C)7→8 | coding (1005/1245 nt) | mtr ← | tryptophan transporter of high affinity |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,815,810 | Δ1 bp | intergenic (‑42/+24) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
RA | 3,951,559 | +A | pseudogene (18/663 nt) | ilvG → | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted |
RA | 3,982,684 | (C)8→10 | noncoding (79/87 nt) | leuT → | tRNA‑Leu |
RA | 4,187,507 | A→C | N720H (AAC→CAC) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,203,611 | A→G | T98A (ACT→GCT) | zraR → | fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
MC JC | 4,397,624 | (GCTGGC)3→2 | coding (213‑218/1848 nt) | mutL → | methyl‑directed mismatch repair protein |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 17 (0.100) | 141 (0.890) | 116/566 | 0.2 | 92.1% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 8 (0.050) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 17 (0.100) | 116 (0.740) | 96/566 | 0.7 | 90.6% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 8 (0.050) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 209 (1.270) | 162/590 | 0.0 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |