breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 88,334 C→T P103S (CCA→TCA)  cra → transcriptional repressor‑activator for carbon metabolism
RA 191,723 C→T intergenic (+15/‑132) tsf → / → pyrH translation elongation factor EF‑Ts/uridylate kinase
RA 240,031 T→C A53A (GCA→GCG yafV ← putative NAD(P)‑binding C‑N hydrolase family amidase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 281,708 T→C G92G (GGA→GGG yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator
RA 300,196 A→G F247L (TTC→CTC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 320,847 G→A R207R (CGG→CGA rclR → reactive chlorine species (RCS)‑specific activator of the rcl genes
RA 323,369 G→A V344V (GTG→GTA ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
RA 538,489 C→T A286V (GCC→GTC)  ybbW → putative allantoin transporter
RA 647,385 A→G V42A (GTG→GCG)  citG ← 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
RA 864,985 A→G Y49H (TAT→CAT)  moeB ← molybdopterin synthase sulfurylase
RA 1,055,493 G→A A229V (GCA→GTA)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,121,967 C→T G214E (GGA→GAA)  pyrC ← dihydro‑orotase
RA 1,145,744 C→T Q269* (CAG→TAG)  rluC → 23S rRNA pseudouridine(955,2504,2580) synthase
RA 1,258,523 T→C intergenic (‑10/‑268) pth ← / → ychH peptidyl‑tRNA hydrolase/DUF2583 family putative inner membrane protein
RA 1,314,127 A→G V36V (GTA→GTG ompW → outer membrane protein W
RA 1,378,342 C→T D153D (GAC→GAT ycjT → putative family 65 glycosyl hydrolase
RA 1,529,218 C→T pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,781,073 G→A G225S (GGC→AGC)  ydiR → putative electron transfer flavoprotein, FAD‑binding subunit
RA 1,831,190 C→T A265T (GCA→ACA)  astC ← succinylornithine transaminase, PLP‑dependent
RA 1,883,341 (G)6→7 coding (154/1446 nt) yeaV → putative transporter
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,047,530 G→A V865I (GTC→ATC)  yeeJ → putative adhesin
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,183,628 (T)7→8 coding (24/819 nt) yegX ← putative family 25 glycosyl hydrolase
RA 2,230,157 G→A L77L (CTG→TTG)  dusC ← tRNA‑dihydrouridine synthase C
RA 2,296,831 G→A A65V (GCG→GTG)  ccmB ← heme export ABC transporter permease
RA 2,515,413 (C)7→8 coding (1083/1113 nt) insL1 → IS186 transposase
RA 2,638,749 T→C S179G (AGT→GGT)  yfgM ← ancillary SecYEG translocon subunit; putative anti‑RcsB factor
RA 2,810,153 Δ1 bp coding (537/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 2,856,252 G→A A639T (GCC→ACC)  fhlA → formate hydrogenlyase transcriptional activator
RA 3,202,425 A→G K179E (AAA→GAA)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,255,057 G→A L5L (TTG→TTA yhaL → uncharacterized protein
RA 3,304,813 (C)7→8 coding (1005/1245 nt) mtr ← tryptophan transporter of high affinity
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,951,559 +A pseudogene (18/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 3,982,684 (C)8→10 noncoding (79/87 nt) leuT → tRNA‑Leu
RA 4,187,507 A→C N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,203,611 A→G T98A (ACT→GCT)  zraR → fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
MC JC 4,397,624 (GCTGGC)3→2 coding (213‑218/1848 nt) mutL → methyl‑directed mismatch repair protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =17 (0.100)141 (0.890) 116/566 0.2 92.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 8 (0.050)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780517 (0.100)116 (0.740) 96/566 0.7 90.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 8 (0.050)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)209 (1.270) 162/590 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein