breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000731 | 81 | N→G | 100% | intergenic (–/‑151) | – / → repA | –/replication protein A |
JC | CP000731 | 6,552 | +28 bp | 100% | intergenic (+1069/‑419) | cadX → / → USA300HOU_pUSA300HOUMR0010 | cadmium efflux regulator/MarR family transcriptional regulator |
RA | CP000731 | 25,663 | Δ1 bp | 100% | coding (316/333 nt) | USA300HOU_pUSA300HOUMR0031 → | hypothetical protein |
JC | USA300TCH1516_ALE | 62,817 | +TCAATT | 100% | intergenic (‑284/+229) | USA300TCH1516_00053 ← / ← speG | hypothetical protein/Spermidine N(1)‑acetyltransferase |
RA | USA300TCH1516_ALE | 111,512 | A→G | 29.0% | S194S (TCA→TCG) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 111,516 | C→A | 29.6% | Q196K (CAA→AAA) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 156,316 | G→A | 14.2% | D135D (GAC→GAT) | treR_1 ← | HTH‑type transcriptional regulator TreR |
RA | USA300TCH1516_ALE | 512,643 | A→G | 48.4% | intergenic (+711/‑6052) | recR → / → speA_2 | Recombination protein RecR/Arginine decarboxylase |
RA | USA300TCH1516_ALE | 994,852 | T→C | 15.5% | M282T (ATG→ACG) | appA → | Oligopeptide‑binding protein AppA |
RA | USA300TCH1516_ALE | 1,018,190 | C→T | 22.5% | intergenic (+115/‑27) | acp → / → xynZ | Sodium/proton‑dependent alanine carrier protein/Endo‑1,4‑beta‑xylanase Z |
RA | USA300TCH1516_ALE | 1,416,458 | A→T | 100% | I216N (ATT→AAT) | oppD_4 ← | Oligopeptide transport ATP‑binding protein OppD |
RA | USA300TCH1516_ALE | 1,513,930 | A→T | 100% | L413F (TTA→TTT) | USA300HOU_RS07375 → | hypothetical protein |
RA | USA300TCH1516_ALE | 1,646,189 | G→C | 100% | A155G (GCA→GGA) | ypdF ← | Aminopeptidase YpdF |
RA | USA300TCH1516_ALE | 1,744,450 | A→T | 27.6% | F74L (TTT→TTA) | apt ← | Adenine phosphoribosyltransferase |
RA | USA300TCH1516_ALE | 1,981,089 | T→G | 41.8% | T105P (ACT→CCT) | USA300HOU_RS09870 ← | hypothetical protein |
RA | USA300TCH1516_ALE | 2,010,363 | C→T | 100% | C146Y (TGT→TAT) | USA300HOU_RS10125 ← | Putative multidrug export ATP‑binding/permease protein |
RA | USA300TCH1516_ALE | 2,279,705 | G→A | 100% | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
JC | USA300TCH1516_ALE | 2,373,573 | Δ9 bp | 16.3% | coding (195‑203/1335 nt) | pbuG ← | Guanine/hypoxanthine permease PbuG |
RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | USA300TCH1516_ALE | 2176690–2181125 | 2181125 | 1–4436 | 6 [5] | [5] 6 | ilvA/USA300TCH1516_02069 | L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | USA300TCH1516_ALE | 512620 = | 27 (1.140) | 17 (1.040) +40 bp |
16/180 | NT | 47.3% | intergenic (+688/‑6075) | recR/speA_2 | Recombination protein RecR/Arginine decarboxylase |
? | USA300TCH1516_ALE | 1998278 = | 28 (1.190) | intergenic (‑6/+3329) | USA300TCH1516_01882/USA300TCH1516_01883 | tRNA‑Val/tRNA‑Ala |