breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 722,162 | C→A | M751I (ATG→ATT) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein |
RA | 1,529,175 | T→C | pseudogene (1274/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,180 | T→C | pseudogene (1279/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,185 | C→T | pseudogene (1284/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,203 | C→A | pseudogene (1302/2037 nt) | rhsE → | pseudogene, Rhs family |
RA | 1,529,218 | C→T | pseudogene (1317/2037 nt) | rhsE → | pseudogene, Rhs family |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,815,810 | Δ1 bp | intergenic (‑42/+24) | pyrE ← / ← rph | orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH |
RA | 4,159,081 | C→T | H198Y (CAC→TAC) | oxyR → | oxidative and nitrosative stress transcriptional regulator |
JC | 4,186,115 | (TTCCGCTGG)1→2 | coding (766/4224 nt) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,233,689 | C→T | intergenic (‑456/‑2218) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
RA | 4,233,762 | C→A | intergenic (‑529/‑2145) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
RA | 4,233,772 | G→T | intergenic (‑539/‑2135) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
RA | 4,235,408 | Δ1 bp | intergenic (‑2175/‑499) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
RA | 4,296,382 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913_3_bme_pgi | 3423706–3424615 | 3424615 | 1–910 | 73 [71] | [71] 74 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913_3_bme_pgi | 1207790 = | 20 (0.150) | 66 (0.570) | 55/222 | 2.2 | 81.2% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913_3_bme_pgi | 1209619 = | 13 (0.110) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913_3_bme_pgi | = 1207805 | 20 (0.150) | 95 (0.820) | 73/222 | 0.7 | 86.2% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913_3_bme_pgi | = 1209602 | 13 (0.110) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913_3_bme_pgi | = 1299498 | 0 (0.000) | 144 (1.120) | 104/246 | 0.1 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913_3_bme_pgi | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913_3_bme_pgi | 3652037 = | NA (NA) | 147 (1.120) | 95/252 | 0.2 | 94.9% | intergenic (+6/+145) | yhiS/insH1 | pseudogene/IS5 transposase and trans‑activator |
? | NC_000913_3_bme_pgi | = 3992709 | 8 (0.060) | coding (1557/2547 nt) | cyaA | adenylate cyclase | |||||
* | ? | NC_000913_3_bme_pgi | = 3653229 | NA (NA) | 124 (0.950) | 96/252 | 0.2 | 96.9% | intergenic (‑31/‑2) | insH1/yhiS | IS5 transposase and trans‑activator/pseudogene |
? | NC_000913_3_bme_pgi | 3992704 = | 4 (0.030) | coding (1552/2547 nt) | cyaA | adenylate cyclase |