Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_bme_pgi 4,235,408 Δ1 bp intergenic (‑2175/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_bme_pgi4,235,4070A.99.4% 845.7 / ‑2.8 177intergenic (‑2174/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (85/90);  minor base T (0/1);  total (85/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

ATCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAC  >  NC_000913_3_bme_pgi/4235258‑4235546
                                                                                                                                                     |                                                                                                                                           
aTCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGa                                                                                                                                             >  3:696517/1‑150 (MQ=255)
 tCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGat                                                                                                                                            <  3:196489/150‑2 (MQ=255)
  cAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGatc                                                                                                                                           >  4:226238/1‑148 (MQ=255)
         ttCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtcg                                                                                                                                   >  3:27489/1‑150 (MQ=255)
              tCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGNGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATg                                                                                                                              <  1:290736/150‑1 (MQ=255)
              tCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACGTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCAGCTGCATGCGCGAAGGAAAGCTTGATCCTCGTCGACATg                                                                                                                              >  2:290736/1‑150 (MQ=255)
               cAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGat                                                                                                                                 <  2:844659/146‑1 (MQ=255)
                    gggCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGcc                                                                                                                        >  3:530885/1‑150 (MQ=255)
                     ggCGTGGAGCTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                                                                       <  4:584465/150‑1 (MQ=255)
                     ggCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCg                                                                                                                       >  3:387359/1‑150 (MQ=255)
                        gTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGAt                                                                                                                    <  1:580781/150‑1 (MQ=255)
                        gTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGAt                                                                                                                    >  2:1381682/1‑150 (MQ=255)
                           gAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                 >  2:1459893/1‑150 (MQ=255)
                            aaCTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAgg                                                                                                                >  2:1219186/1‑150 (MQ=255)
                             aCTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                               >  2:1274982/1‑150 (MQ=255)
                              cTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                                                              <  4:696517/150‑1 (MQ=255)
                               tGGGTAAGGAAGTGGCGAGGGAAGTTTTGCGGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTCATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                             <  2:873035/150‑1 (MQ=255)
                                 ggTAAGGAACTGGCGACGGCACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTc                                                                                                           >  4:378549/1‑150 (MQ=255)
                                   tAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                             >  2:126362/1‑146 (MQ=255)
                                   tAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                             <  1:126362/146‑1 (MQ=255)
                                    aaGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGa                                                                                                                                  <  4:667459/124‑1 (MQ=255)
                                    aaGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGa                                                                                                                                  >  3:667459/1‑124 (MQ=255)
                                     aGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                       <  2:1332480/150‑1 (MQ=255)
                                      ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                      <  1:756808/150‑1 (MQ=255)
                                      ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                      <  1:1459893/150‑1 (MQ=255)
                                      ggAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                      >  3:837129/1‑150 (MQ=255)
                                       gAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccc                                                                                                     >  4:349652/1‑149 (MQ=255)
                                            ggCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                >  4:427729/1‑150 (MQ=255)
                                                       ttttGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                                                                     >  1:1137174/1‑150 (MQ=255)
                                                            ccGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGTTGCCTGa                                                                                >  3:882228/1‑150 (MQ=255)
                                                             cGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTTCACGCAAGGGCTTGTCGCGCATCTGCATGCACGAAGGAAAGTTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                               <  1:251446/150‑1 (MQ=255)
                                                             cGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGa                                                                                                                                  >  4:546664/1‑99 (MQ=255)
                                                              ggTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                                                              >  1:1097810/1‑150 (MQ=255)
                                                                   tATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                         <  1:1048756/150‑1 (MQ=255)
                                                                       gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGatat                                                                                                                               >  4:405140/1‑92 (MQ=255)
                                                                       gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                             >  1:359155/1‑126 (MQ=255)
                                                                       gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                             <  2:359155/126‑1 (MQ=255)
                                                                       gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                     >  3:927021/1‑150 (MQ=255)
                                                                       gggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTCATGCGACGCGGt                                                                     >  4:891604/1‑150 (MQ=255)
                                                                        ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                    >  1:387881/1‑150 (MQ=255)
                                                                        ggCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                    >  1:19073/1‑150 (MQ=255)
                                                                          cAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                  <  1:1183682/150‑1 (MQ=255)
                                                                           aaggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                                                            >  2:1536259/1‑107 (MQ=255)
                                                                            aggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGAcgcgaccgcgt                                                                <  2:1143175/150‑8 (MQ=39)
                                                                             ggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                               >  4:776772/1‑150 (MQ=255)
                                                                             ggaaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                               <  4:117362/150‑1 (MQ=255)
                                                                               aaggGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                             <  1:1381682/150‑1 (MQ=255)
                                                                                 ggggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGTAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                             >  2:956147/1‑148 (MQ=255)
                                                                                 ggggCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                           <  4:620014/150‑1 (MQ=255)
                                                                                         tGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa                                                   >  2:561087/1‑150 (MQ=255)
                                                                                           gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGt                                                                                                                             <  2:825475/74‑1 (MQ=255)
                                                                                           gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGt                                                                                                                             >  1:825475/1‑74 (MQ=255)
                                                                                           gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                      >  2:763859/1‑145 (MQ=255)
                                                                                           gTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                      <  1:763859/145‑1 (MQ=255)
                                                                                            tCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGcc                                                                                                                        <  2:1091120/78‑1 (MQ=255)
                                                                                            tCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                    <  1:33045/114‑1 (MQ=255)
                                                                                            tCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                <  1:1537532/150‑1 (MQ=255)
                                                                                            tCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                                >  2:570451/1‑150 (MQ=255)
                                                                                              gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCAtcgtc                                                                                                                                    >  1:849902/1‑64 (MQ=255)
                                                                                              gcgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                              <  4:435294/150‑1 (MQ=255)
                                                                                               cgACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACGGGTc                                             >  2:960543/1‑150 (MQ=255)
                                                                                                  cGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAt                                          <  2:28511/150‑1 (MQ=255)
                                                                                                    cTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGTATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                        <  1:929058/150‑1 (MQ=255)
                                                                                                      tCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg                                                       <  2:137048/133‑1 (MQ=255)
                                                                                                      tCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                      >  2:778785/1‑150 (MQ=255)
                                                                                                       ccACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                     <  3:427729/150‑1 (MQ=255)
                                                                                                        cACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                    >  2:138738/1‑150 (MQ=255)
                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                          <  1:159948/127‑1 (MQ=255)
                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                          >  2:159948/1‑127 (MQ=255)
                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAt                                          >  3:75757/1‑143 (MQ=255)
                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAt                                          <  4:75757/143‑1 (MQ=255)
                                                                                                         aCGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                   >  4:918277/1‑150 (MQ=255)
                                                                                                            cAAGGGTTTGTCGCGCACTTGCACGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                <  2:593935/150‑1 (MQ=25)
                                                                                                            cAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACACGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGt                                >  1:1433626/1‑150 (MQ=255)
                                                                                                               gggCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                             <  2:250065/150‑1 (MQ=255)
                                                                                                                 gCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATCTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                           <  4:837129/150‑1 (MQ=255)
                                                                                                                  cTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                          <  3:886339/150‑1 (MQ=255)
                                                                                                                     gTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                             <  1:1039287/128‑1 (MQ=255)
                                                                                                                     gTCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc                                             >  2:1039287/1‑128 (MQ=255)
                                                                                                                      tCGCGCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                      <  3:918277/150‑1 (MQ=255)
                                                                                                                       cgcgCATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                     <  2:919182/150‑1 (MQ=255)
                                                                                                                         cgcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                   <  1:138738/150‑1 (MQ=255)
                                                                                                                         cgcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                   <  1:1301605/150‑1 (MQ=255)
                                                                                                                          gcATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc                  <  1:570451/150‑1 (MQ=255)
                                                                                                                           cATCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                       <  2:109808/80‑1 (MQ=255)
                                                                                                                            aTCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                <  1:778785/150‑1 (MQ=255)
                                                                                                                             tCTGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt               <  2:19073/150‑1 (MQ=255)
                                                                                                                               tGCATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTa             <  3:104156/150‑1 (MQ=255)
                                                                                                                                 cATGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt           <  3:613656/150‑1 (MQ=255)
                                                                                                                                  aTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa          >  2:1555930/1‑150 (MQ=255)
                                                                                                                                  aTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa          <  1:1536030/150‑1 (MQ=255)
                                                                                                                                  aTGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa          >  2:496002/1‑150 (MQ=255)
                                                                                                                                   tGCGCGAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCAATATGTACATCGTATTCGGCAATTAATAc         >  4:900927/1‑150 (MQ=255)
                                                                                                                                      gcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt           >  1:320098/1‑145 (MQ=255)
                                                                                                                                      gcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt           <  2:320098/145‑1 (MQ=255)
                                                                                                                                      gcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCAGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                              <  4:462377/126‑1 (MQ=255)
                                                                                                                                      gcgAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCAGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                              >  3:462377/1‑126 (MQ=255)
                                                                                                                                        gAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                     <  4:184011/117‑1 (MQ=255)
                                                                                                                                        gAAGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                     >  3:184011/1‑117 (MQ=255)
                                                                                                                                         aaGGAAAGCTTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa   <  2:620328/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
ATCAATGCCTTCGATCAATGGGGCGTGGAACTGGGTAAGGAACTGGCGACGGAACTTTTGCCGGTTGTATCGGGCAAGGAAGGGGCGAGTGGTCGCGACGCTTCCACGCAAGGGCTTGTCGCGCATCTGCATGCGCGAAGGAAAGCTTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCAC  >  NC_000913_3_bme_pgi/4235258‑4235546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: