Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	4,001,644	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001643	1 (0.010)	53 (0.790)	46/284	0.4	98.2%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001649 =	1 (0.010)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001504‑4001643 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCA  >  NC_000913/4001649‑4001789                                                                                                                                                                                                                                                                                           TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTC                                                                                                                                            >  2:641176/1‑143      ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCT                                                                                                                                       >  2:317220/1‑143      ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACACCGACGAATCGGAACATTACGTTGAAGACGACGACGGCCTGCATCTCCTTCT                                                                                                                                       <  1:413156/143‑1             TTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTGCTTCTcact                                                                                                                                >  1:1124150/1‑139              TGTCGAACCGCACGACGACGAGCGACTGCGCGTACAAGCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTT                                                                                                                               <  2:66023/143‑1               gcCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTG                                                                                                                              <  2:643208/141‑1                 CGACCCGGACGACGACGCGCGACCGCGCGTACAAGCTGACCTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGCAAGACATCGAAGCATCGGCACGTTTCTTTGTAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAA                                                                                                                            <  1:441523/143‑1                       GGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTCCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGCTGCG                                                                                                                      >  1:584372/1‑143                          cgccgtctacCGACTGCGCGACCAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAA                                                                                                                   <  1:336286/133‑1                              GACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATC                                                                                                               >  2:193474/1‑143                                 GAGCGACTGCGCGTACAATCTGGTCTTGGCCAGGGGCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACG                                                                                                            <  1:95863/143‑1                                 GAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGCTGCGGCAGATCACG                                                                                                            >  2:1027338/1‑143                                  AGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAGGC                                                                                                           <  2:682433/143‑1                                    cgcCTGCGCGTACAATCTGACCTTGGCCTGGTTCTGCCACCCCCCCCTGAACTGTAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACAACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCG                                                                                                         <  2:157797/140‑1                                    CGACTGCGCGTACAATCTGAACTTGGCCCGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCG                                                                                                         <  1:1023049/143‑1                                      ACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCGCGCCGGT                                                                                                       >  1:211308/1‑143                                        TGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGCAGATCACGCCGGTAA                                                                                                     >  1:37592/1‑143                                         GCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAAC                                                                                                    >  1:62329/1‑143                                          gGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAGCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACT                                                                                                   <  1:198919/142‑1                                              TACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGGAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCAC                                                                                               >  2:943530/1‑143                                              TACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCAC                                                                                               >  2:943578/1‑143                                                 AATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGT                                                                                            <  2:771908/143‑1                                                               CAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCC                                                                              >  2:346345/1‑143                                                                    CCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATGACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAT                                                                         >  2:811030/1‑143                                                                    CCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCGGTGAT                                                                         >  2:811024/1‑143                                                                     CTGGCAGCCCGGCCGGACCTGGAAGGCATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATG                                                                        <  1:79715/143‑1                                                                     CTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATG                                                                        <  2:503794/143‑1                                                                     CTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCGTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCGCGCCGAGAACGCCACTGTGGCATGTACCATCCGTGCTG                                                                        >  2:289922/1‑143                                                                      TGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTGTGCGAGGATGCGGAAGCTCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGG                                                                       >  2:376440/1‑143                                                                              CGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGT                                                               <  2:1099332/143‑1                                                                                 CCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTA                                                            <  2:884586/143‑1                                                                                     acccGGAAGACATCGAAGCCTCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCT                                                        <  2:24013/139‑1                                                                                       caacAAGACGTCGACGCATCGGGACGCTTCCTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTACCTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGC                                                      <  2:256530/139‑1                                                                                          GAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCCGTAACTCCACTGTCGCATTTACCATCCGCGCTGGTCGCCGGTGTCCTCTGCGTG                                                   >  1:716160/1‑143                                                                                            AGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATGCGTGATGGTCGGATGTTTACTCTGCGTGAG                                                 >  2:182084/1‑143                                                                                               CATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCTGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGT                                              <  2:734959/143‑1                                                                                                ATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTCGCAGATGCGGCAGATCACGCCGGTCACTCCACTGTGGCATTTACCATCCGTACTGGCCGCCTCTTTACTCTGCGTCAGCctc                                             >  1:794252/1‑140                                                                                                ATCGAAGCATCGGCACGTTTCTTTGAAGACGAAGACGGCTTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTG                                             >  2:50129/1‑143                                                                                                 TCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGCCTGTTTACTCTGCGTGCCCGTGA                                            >  2:282304/1‑143                                                                                                 TCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGA                                            >  2:139588/1‑143                                                                                                  CGAAGCATGGGCACGTTTCTTTGAAGACGACGGCGGCCTGCAGCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAA                                           <  1:213165/143‑1                                                                                                      GCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGC                                       <  2:74906/143‑1                                                                                                           GGCACGTTTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGCAGATGCGGAAGCTAGGGGGGGTAACTCCACTGTGGCGTTTACCCTCCGTGCTGGTCGTCTGTTTACTCTGCGTGAGCGGGCGCTGCCCGCT                                  >  2:1159248/1‑143                                                                                                                 TTTCGTTGCAGACGACGACGGCCTGCCTCTCTTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTATCTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGT                            <  1:809257/143‑1                                                                                                                  TTCTTTGCAGACGACGACGGTCTGCATCTCCTTCTTCTTCTTTGCAGCTGCGGAAGATCCCGCCGGTAACTCCACTGTGGCATTTACCATCCGTGCTGGTCGAACATACACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                           <  1:139588/143‑1                                                                                                                  TTCTTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTC                           >  2:4886/1‑143                                                                                                                     TTTGAAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGCTGGTCGTCTCTTTCCTCTGCGTGAGCGTCCACTGCCCGCTTTcccgccgt                        >  2:295436/1‑135                                                                                                                         AAGACGACGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGGGAACTGCGCGCTTTTCGGCTGTATCG                    >  2:723586/1‑143                                                                                                                            ACGACGACGGCCTGCATCTCCTTCATCTTCTTGGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAT                 <  2:211308/143‑1                                                                                                                                CGACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGGATGGTCGTCTGATTAGTCTGCGTGAGCGTGAAATGCCGGCTTTTGGTCTGTAGCGACTGCGc             >  1:1034181/1‑142                                                                                                                                 GACGGCCTGCATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACGATCCGTGATGGTCGTCTGTTTACTCTGCGTGCGCGGGAACTGCCCTCTCTTCGTCTGTATCCTACGCGgc            >  1:922488/1‑141                                                                                                                                  ACGGCCTGTATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGC           <  1:176080/143‑1                                                                                                                                           ATCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATCCCCGTCGGGATACTTTGAGTGCGACAGAACTGCCCGCTTTTCGTCTGTGTCGTGTGCGTGCCCGTAGCCA  >  1:825428/1‑143                                                                                                                                                                                                                                                                                           TATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4001504‑4001643 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCA  >  NC_000913/4001649‑4001789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.