BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	117 [0]	[0] 119	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	110 (1.530)	76/274	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
*	?	NC_000913	1300698 =	0 (0.000)	110 (1.530)	76/274	0.0	100%	intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Only 100 of 119 total aligned reads displayed.

ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300557‑1300840

attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaat                                                                                                                                                <  1:540052‑M2/2‑1 (MQ=255)

 ttacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaata                                                                                                                                               <  2:860245‑M2/3‑1 (MQ=255)

    cagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                            <  2:746660‑M2/6‑1 (MQ=255)

     agttagttataaggatttccttaactgcttctcctccccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                           <  2:145065‑M2/7‑1 (MQ=255)

     agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccatatcattatcctgggattgctgaagaataattgaaatgatattatttattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                           <  1:326721‑M2/7‑1 (MQ=255)

     agttagttataaggatttccttaactgctagtcctgaccatcatgttatttttgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                           <  1:315087‑M2/7‑1 (MQ=255)

              taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgacgaataattgaaatgatattattaattccactgccttttgtagatgaaagtgctacATACTTATCAATTGtt                                                                                                                                  >  2:630293‑M2/128‑143 (MQ=255)

              taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtataggaaagtgctaaATAATAATCAATTGtt                                                                                                                                  >  1:1039819‑M2/128‑143 (MQ=255)

               aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTa                                                                                                                                 >  2:269983‑M2/127‑143 (MQ=255)

                aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATtgttta                                                                                                                                <  2:479359‑M2/18‑3 (MQ=255)

                 gtctttccttaactgcttctcctcaccatcatgttactttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                               <  1:1293388‑M2/19‑1 (MQ=255)

                    attgcttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                            >  2:944014‑M2/122‑143 (MQ=255)

                     ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                           >  2:1175093‑M2/121‑143 (MQ=255)

                     ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                           >  2:905979‑M2/121‑143 (MQ=255)

                     ttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAAACAATGGTTAAattat                                                                                                                           >  2:170672‑M2/121‑143 (MQ=255)

                      tccttaactgttgctcctcaccatcatgttattttcgccacatcatactcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                          <  1:1172997‑M2/24‑1 (MQ=255)

                      tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                          <  1:1222031‑M2/24‑1 (MQ=255)

                      tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                          <  2:359269‑M2/24‑1 (MQ=255)

                      tcctgaactgcttctcctcaccatcatgttattttcgccacatcataatccttggcttgctgaagtataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                          <  1:617640‑M2/24‑1 (MQ=255)

                        cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgacgaatacttgaaatgatattattaattccactccctttgatagagaaaagtgctaaATAATAATAAATTGTTAAATTATtgt                                                                                                                        >  2:1251303‑M2/118‑143 (MQ=255)

                         ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                       <  2:396660‑M2/27‑1 (MQ=255)

                         ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                       >  2:556868‑M2/117‑143 (MQ=255)

                          taactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGc                                                                                                                      <  1:707326‑M2/28‑1 (MQ=255)

                            atggcttctcctccccatcatgttatttccgccacatcataatcctgggcttgctgaagaataattgaaatgatattatttattccactggctttggtagaggaaagtgctaaTTAATAATCAATTGTTAAATTATTGTGCAt                                                                                                                    <  2:951549‑M2/30‑1 (MQ=255)

                              tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                  <  2:835739‑M2/32‑1 (MQ=255)

                              tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                  >  1:451006‑M2/112‑143 (MQ=255)

                                cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattacactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                                >  1:1120615‑M2/110‑143 (MQ=255)

                                cctctcctcaccttcattttattttcgccacatcataatccttggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                                <  1:514279‑M2/34‑1 (MQ=255)

                                 ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                               >  2:1098635‑M2/109‑143 (MQ=255)

                                     cctctccgtcatgttattttcgtcacatcataaccctgggcttgctgaagaataattgaaatgatattattaattccaatgactttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                           <  1:773961‑M2/39‑1 (MQ=255)

                                     cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                           >  1:485001‑M2/105‑143 (MQ=255)

                                      ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagagggaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                          >  2:1081066‑M2/104‑143 (MQ=255)

                                       tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTTTTGTGCATTTCACTTCTgg                                                                                                         >  2:197854‑M2/103‑143 (MQ=255)

                                         accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                       <  1:928153‑M2/43‑1 (MQ=255)

                                           cgtcaggttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                     <  2:76539‑M2/45‑1 (MQ=255)

                                           catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                     >  2:579623‑M2/99‑143 (MQ=255)

                                           catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                     <  2:711046‑M2/45‑1 (MQ=255)

                                            atcatgttattttcgccacatcataatcctgggcttgctgaagcataattgaaatgctattattaattccactgcctttggtagaggacagtgctacATAATAATCAATTGTTACATTATTGTGCATTTCTCTCCTGGgaccg                                                                                                    >  2:331451‑M2/98‑141 (MQ=255)

                                             tcatgttattttcgcaatatcagaatcctgggcttgctgcagaataattgaaatgatattattaattccactgcctttgatagaggaaagtgctaaTTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                                   <  1:1169748‑M2/47‑1 (MQ=255)

                                                tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                >  2:25606‑M2/94‑143 (MQ=255)

                                                tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgactgtggtagaggaaagtgctaaATAATAATCAATTGTTAAATCATTGTGCATTACACTACTGGAACTGTAAt                                                                                                >  1:622976‑M2/94‑143 (MQ=255)

                                                 gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                               <  1:1178336‑M2/51‑1 (MQ=255)

                                                           ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggacagtgctaaCTAATAATCAATTGTTAATTTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑a                                                                                    >  2:130853‑M2/83‑143 (MQ=255)

                                                           ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑a                                                                                    >  2:818359‑M2/83‑143 (MQ=255)

                                                              catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGAc                                                                                 >  2:1198352‑M2/80‑143 (MQ=255)

                                                              catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGAc                                                                                 <  1:954028‑M2/64‑1 (MQ=255)

                                                               atcataatcctgggcttgctgaagaataattgaaatgatattattaattcccctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTAGAACTGTAATCAGAAACGAT‑AGACa                                                                                >  1:862258‑M2/79‑143 (MQ=255)

                                                                  atattccttggcttgttgaagaataattgaaatgatattagtgattccactgctttttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAGTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGc                                                                             <  1:704269‑M2/68‑1 (MQ=255)

                                                                    aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCtt                                                                           >  2:1059802‑M2/74‑143 (MQ=255)

                                                                     atcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTa                                                                          <  1:1010038‑M2/71‑1 (MQ=255)

                                                                     accctgtgcttgctgaatactaaatgaaatgatattatcaattccactgcctttggtagaggaaagtgctaaATTATAATCAATTGTTAACTTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTa                                                                          <  1:237663‑M2/71‑1 (MQ=255)

                                                                      tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagagctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACAACTGGAACAGTAATCAGTAAAGAT‑AGACACTCTTAg                                                                         >  1:606277‑M2/72‑143 (MQ=255)

                                                                         tgggcttgctgaataataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCa                                                                      <  1:63188‑M2/75‑1 (MQ=255)

                                                                            gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAAtc                                                                   >  1:1212905‑M2/66‑143 (MQ=255)

                                                                             cttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAAtct                                                                  <  1:1243727‑M2/79‑1 (MQ=255)

                                                                                gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAACTTATTGTGCATTTCACTACTGGCACTGTAATCAGAAAAGAT‑AAACATGCTTAGCCAATCTCTa                                                               >  2:632496‑M2/62‑143 (MQ=255)

                                                                                  tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTAtt                                                             <  2:675137‑M2/84‑1 (MQ=255)

                                                                                  tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTAtt                                                             >  2:655978‑M2/60‑143 (MQ=255)

                                                                                  tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGAAATGCTTAGCCAATCTCTCtt                                                             >  1:650148‑M2/60‑143 (MQ=255)

                                                                                    aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCATTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATttg                                                           >  2:287893‑M2/58‑143 (MQ=255)

                                                                                    aagaataattgaaatgatattattaattccactgcctgtggtagaggaaagtgctaaATAATAATCAATAGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAG‑AGACATGCGTAGCCAGTCTCTAtgtg                                                           >  2:254982‑M2/58‑143 (MQ=255)

                                                                                     agaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATttga                                                          <  1:341649‑M2/87‑1 (MQ=255)

                                                                                     agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATttga                                                          <  1:1041763‑M2/87‑1 (MQ=255)

                                                                                      gtgtctgtcaaaagagattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATttgat                                                         <  1:1175093‑M2/88‑1 (MQ=255)

                                                                                           attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGattgaat                                                    >  2:850441‑M2/51‑143 (MQ=255)

                                                                                                aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCAATTTGATTGAATTGaaa                                               >  2:92662‑M2/46‑143 (MQ=255)

                                                                                                      attattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTACTCCGAAAAGAT‑AGACATGCTTAGCCAATATCTCTTTAATTGAATTGAAAGAggtt                                         >  1:527225‑M2/40‑143 (MQ=255)

                                                                                                       ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  2:634454‑M2/105‑1 (MQ=255)

                                                                                                       ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  2:1087802‑M2/105‑1 (MQ=255)

                                                                                                       ttattaatcccactgcctgtgggagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  2:26063‑M2/105‑1 (MQ=255)

                                                                                                       gtattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                        <  1:1292382‑M2/105‑1 (MQ=255)

                                                                                                         attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                      <  1:1198352‑M2/107‑1 (MQ=255)

                                                                                                          ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑CGCCATGCTTAGCCAATATCTCTTTGATTGAATTGAAAGAtgtttgtt                                     <  2:953851‑M2/108‑1 (MQ=255)

                                                                                                          ttaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgtt                                     >  2:516023‑M2/36‑143 (MQ=255)

                                                                                                            aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                   <  2:323120‑M2/110‑1 (MQ=255)

                                                                                                            aatgccactgcctttggtagaggaaagtgctaaATAAGAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                   <  1:167411‑M2/110‑1 (MQ=255)

                                                                                                             attccactgctgttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                  <  1:943976‑M2/111‑1 (MQ=255)

                                                                                                                ccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGGATTGACAGATGTTCGTTACGGCa                               >  1:1200702‑M2/30‑143 (MQ=255)

                                                                                                                 cttttttgtttgtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  1:1106815‑M2/115‑1 (MQ=255)

                                                                                                                 cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  2:794023‑M2/115‑1 (MQ=255)

                                                                                                                 cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGACAt                              >  1:1096553‑M2/29‑143 (MQ=255)

                                                                                                                 cactgcccttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                              <  2:1064002‑M2/115‑1 (MQ=255)

                                                                                                                    tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                           >  2:173103‑M2/26‑143 (MQ=255)

                                                                                                                    tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACACGCTTAGCCAACCACTATTTGATTGAATTGAAAGATGTTCGTTCAGGCATGGa                           >  1:1189010‑M2/26‑143 (MQ=255)

                                                                                                                      cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATg                         >  2:129631‑M2/24‑143 (MQ=255)

                                                                                                                       ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTAGAACTGTAATAAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                        >  1:833604‑M2/23‑143 (MQ=255)

                                                                                                                             tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAGGctc                  >  2:547773‑M2/17‑142 (MQ=255)

                                                                                                                                  gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt             >  1:233243‑M2/12‑143 (MQ=255)

                                                                                                                                    aagtgctaaATAATAATCAATTGTTAAATTGTTGTGGACAACACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  1:908310‑M2/134‑1 (MQ=255)

                                                                                                                                    aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  2:661704‑M2/134‑1 (MQ=255)

                                                                                                                                     agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt          >  1:772298‑M2/9‑143 (MQ=255)

                                                                                                                                     agtgctaaATAACAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATACAAGCTATAGATTCt          <  2:1182332‑M2/135‑1 (MQ=255)

                                                                                                                                      gtgctaaATCATAGTCAATTGTTAAATTATTGTGCATTTCACTACTGGATCTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTg         <  1:268218‑M2/136‑1 (MQ=255)

                                                                                                                                       tgctccataatactCAATTGTTAAAGTATTGTGCATTTCACTACTGGCACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa        <  1:197854/137‑1 (MQ=255)

                                                                                                                                         ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa      <  2:1212905‑M2/139‑1 (MQ=255)

                                                                                                                                          taaATAATAATTAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCCGGTTTG‑AAAGCCAGTCTCTATTTGATTCAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     <  2:947003‑M2/140‑1 (MQ=255)

                                                                                                                                          taaATAATAATCAATTGTTAAATTTTTGTGGTTGTAAATACTAAAACGGGAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc     <  2:534879‑M2/140‑1 (MQ=255)

                                                                                                                                          taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAtaa     >  1:754275‑M2/4‑142 (MQ=255)

                                                                                                                                            aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAACTATAGATTCTGATACgg   <  2:689663‑M2/142‑1 (MQ=255)

                                                                                                                                             ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  1:29311/143‑1 (MQ=255)

ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAT‑AGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300557‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`