Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,335,153	Δ593 bp		[ymiA]–yciX	[ymiA], yciX

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1335153	1335745	593	53 [0]	[0] 51	[ymiA]–yciX	[ymiA],yciX

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1335152	0 (0.000)	51 (0.570)	45/282	0.9	100%	coding (29/165 nt)	ymiA	uncharacterized protein
	?	NC_000913	1335746 =	0 (0.000)					intergenic (+288/‑85)	yciX/acnA	uncharacterized protein/aconitate hydratase 1; aconitase A

TTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGT  >  NC_000913/1335004‑1335299                                                                                                                                                     |                                                                                                                                                    tttCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAg                                                                                                                                                     <  8:360584/149‑1 (MQ=255) tttCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAg                                                                                                                                                     <  8:303042/149‑1 (MQ=255)     aGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaat                                                                                                                                                 >  5:336896‑M1/1‑145 (MQ=255)                 tGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGAGATGGCGATTATATGAGaaatcgttacgcgatg                                                                                                                                     >  8:37391‑M1/1‑133 (MQ=255)                       attCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgt                                                                                                                                <  5:248638‑M1/148‑22 (MQ=255)                        ttCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtg                                                                                                                               <  5:210493‑M1/148‑23 (MQ=255)                           aCTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaagtcgttgcgggagggttgtgtgtg                                                                                                                           >  6:265069‑M1/1‑123 (MQ=255)                               gagaCGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATTTGAGaaatcgttacgcgatgtttgtgttatctt                                                                                                                        >  4:214791‑M1/1‑119 (MQ=255)                               gagaCGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctt                                                                                                                        <  6:336896‑M1/148‑30 (MQ=255)                                 gaCGATTCGTAAACTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaa                                                                                                                     <  7:198610‑M1/149‑33 (MQ=255)                                    gATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatat                                                                                                                  >  5:146695‑M1/1‑114 (MQ=255)                                    gATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatat                                                                                                                  >  8:37557‑M1/1‑114 (MQ=255)                                       tCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattca                                                                                                               >  8:348519‑M1/1‑111 (MQ=255)                                        cGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattca                                                                                                               <  1:382336‑M1/148‑39 (MQ=255)                                         gTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcacc                                                                                                             >  8:195189‑M1/1‑109 (MQ=255)                                         gTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgt                                                                                                                                    <  3:222525‑M1/126‑18 (MQ=255)                                         gTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgt                                                                                                                                    >  4:222525‑M1/1‑109 (MQ=255)                                           aaaTTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccct                                                                                                           >  5:297039‑M1/1‑107 (MQ=255)                                            aaTTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccct                                                                                                           <  7:195189‑M1/148‑43 (MQ=255)                                             aTTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctga                                                                                                         <  5:265069‑M1/149‑45 (MQ=255)                                              ttCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaa                                                                                                        <  7:348519‑M1/149‑46 (MQ=255)                                              ttCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaa                                                                                                        <  5:298906‑M1/149‑46 (MQ=255)                                                       tttttGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACTTCTTGCTGATAAGTGATGGCGATTATATGTGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcag                                                                                               <  5:130243‑M1/149‑55 (MQ=255)                                                       tttttGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcag                                                                                               <  7:127414‑M1/149‑55 (MQ=255)                                                                  aGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaac                                                                                    <  6:297039‑M1/149‑66 (MQ=255)                                                                      tCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccct                                                                                 <  1:320964‑M1/148‑69 (MQ=255)                                                                          tAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtca                                                                             <  6:146695‑M1/148‑73 (MQ=255)                                                                          tAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgt                                                                               <  8:247223‑M1/146‑71 (MQ=255)                                                                           aGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatcttttatattcaccctgaagagaatcagggcttcgcaaccctgtcat                                                                            >  2:309340‑M1/1‑75 (MQ=255)                                                                             gATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgagatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcatt                                                                           >  1:105894‑M1/1‑73 (MQ=255)                                                                                     tcATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagc                                                                 >  7:174695‑M1/1‑65 (MQ=255)                                                                                          gTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgt                                                            >  4:57676‑M1/1‑60 (MQ=255)                                                                                              gATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgt                                                         >  4:88163‑M1/1‑56 (MQ=255)                                                                                                ttaattaCACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaa                                                      >  2:14051‑M1/1‑54 (MQ=255)                                                                                                ttaattaCACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtc                                                           >  2:321407‑M1/1‑54 (MQ=255)                                                                                                ttaattaCACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtc                                                           <  1:321407‑M1/144‑91 (MQ=255)                                                                                                           aaTCGTTCAACGTCTTGCTGATATGTGATGGCGATTATATGAGatatcgtttcgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaacccagtcattaaggaggagctatgtcgtcaaccctacgag                                             <  8:101530‑M1/147‑105 (MQ=255)                                                                                                           aaTCGTTCAACCTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgaagtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggagtagctatgtcgtcaaccctacgag                                             >  7:101530‑M1/1‑43 (MQ=255)                                                                                                                ttCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagcta                                                               <  2:193723‑M1/124‑87 (MQ=255)                                                                                                                   aaCGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagt                                     >  2:133490‑M1/1‑35 (MQ=255)                                                                                                                    aCGTCTTGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaag                                  <  1:133490‑M1/149‑116 (MQ=255)                                                                                                                         ttGCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtacaccatacgagaagccagtaaggacac                             >  4:325445‑M1/1‑29 (MQ=255)                                                                                                                           gCTGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgt                           >  1:147597‑M1/1‑27 (MQ=255)                                                                                                                             tGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttg                         >  6:170717‑M1/1‑25 (MQ=255)                                                                                                                             tGATAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttg                         >  2:308652‑M1/1‑25 (MQ=255)                                                                                                                               aTAAGTGATGGAGATTATATGATaaatcgttacgcgatgtttgttttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttg                         <  2:105894‑M1/147‑125 (MQ=255)                                                                                                                                tAAGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcag                      <  7:37557‑M1/149‑128 (MQ=255)                                                                                                                                  aGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcaggc                    >  4:75328‑M1/1‑20 (MQ=255)                                                                                                                                  aGTGATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcaggc                    <  3:88163‑M1/149‑130 (MQ=255)                                                                                                                                     gATGGCGATTATATGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcaggcca                  <  1:309340‑M1/148‑132 (MQ=255)                                                                                                                                                tatGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcaggccaaagataaaact       <  1:308652‑M1/148‑143 (MQ=255)                                                                                                                                                 atGAGaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttcgcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcaggccaaagataaaactta     >  1:344488‑M1/1‑5 (MQ=255)                                                                                                                                                    agaaatcgttacgcgatgtttgtgttatctttaatattcaccctgaagagaatcagggcttagcaaccctgtcattaaggaggagctatgtcgtcaaccctacgagaagccagtaaggacacgttgcaggccaaagctaaaacttcccc  >  4:57735‑M1/1‑2 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTTCAGGTCAAAAGATTGAATTATTCACTGGAGACGATTCGTAAATTCGCTGGATTTTTGACTAAAGTTTCTTTAGGATTTGTCTCATCGTATGATTAATTACACCAATCGTTCAACGTCTTGCTGATAAGTGATGGCGATTATATGAGGTTAGCAATGCCTTCTGGAAATCAGGAACCCCGCCGCGATCCTGAATTAAAACGTAAAGCCTGGCTGGCGGTTTTTCTTGGTTCTGCACTTTTCTGGGTGGTTGTCGCACTGCTGATTTGGAAAGTGTGGGGATAACTATGGTCGGT  >  NC_000913/1335004‑1335299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑