Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_pgi	3,440,077	Δ11 bp	coding (943‑953/990 nt)	rpoA ←	RNA polymerase, alpha subunit

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_pgi	3440077	3440087	11	70 [0]	[0] 70	rpoA	RNA polymerase, alpha subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_pgi	= 3440076	0 (0.000)	68 (1.160)	56/274	0.1	100%	coding (954/990 nt)	rpoA	RNA polymerase, alpha subunit
	?	NC_000913_3_pae_pgi	3440088 =	0 (0.000)					coding (942/990 nt)	rpoA	RNA polymerase, alpha subunit

ATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGC  >  NC_000913_3_pae_pgi/3439941‑3440236                                                                                                                                                    |                                                                                                                                                     gattgcggaaaatagcctggcgatggctgctgttgcgtttcagttgacgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCa                                                                                                                                                     <  2:174302‑M2/2‑1 (MQ=255)          acatagcctggcgatggctgctgttgcggttcagttgacgaccactcttacgatggcgcatgaccttatccttctcagtaaaacccttacctttcataagcagccagggcagcgatgcttgccggtggccagttttccCAGAGACAGTc                                                                                                                                            <  1:780070‑M2/11‑1 (MQ=255)               gcctggcgatggctgctgttgcggttcagttgacgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACgg                                                                                                                                       <  1:375020‑M2/16‑1 (MQ=255)                         ggctgctgttgcggttcagttgacgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCa                                                                                                                             <  2:109548‑M2/26‑1 (MQ=255)                          gctgctgttgcggttcagttgacgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCAc                                                                                                                            >  1:962232‑M2/123‑149 (MQ=255)                            tgctgttgcggttcagttgacgaccactcttacgatggcgcatgacgttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTGCACGGGAAGCCAGCACGt                                                                                                                          >  1:6707‑M2/121‑149 (MQ=255)                                 ttgcggttcagttgacgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTa                                                                                                                     <  2:962232‑M2/34‑1 (MQ=255)                                              gacgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAagag                                                                                                        <  1:347777‑M2/47‑1 (MQ=255)                                               acgaccactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCCCAGTAAgaga                                                                                                       >  1:50991‑M2/102‑149 (MQ=255)                                                 gaccacttgtacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtt                                                                                                     <  2:767085‑M2/50‑1 (MQ=255)                                                  accactcttacgatggtgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttt                                                                                                    <  2:837828‑M2/51‑1 (MQ=255)                                                  accactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACgg                                                                                                                                       <  1:676249‑M2/16‑1 (MQ=255)                                                  accactcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACgg                                                                                                                                       >  2:676249‑M2/99‑114 (MQ=255)                                                       tcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                               <  2:973894‑M2/40‑1 (MQ=255)                                                       tcttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                               >  1:973894‑M2/94‑133 (MQ=255)                                                        cttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAg                                                                                                                               <  2:678834‑M2/24‑1 (MQ=255)                                                        cttacgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAg                                                                                                                               >  1:678834‑M2/93‑116 (MQ=255)                                                           acgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTa                                                                                                <  2:859417‑M2/55‑1 (MQ=255)                                                           acgatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTa                                                                                                >  1:859417‑M2/90‑144 (MQ=255)                                                             gatggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGGCTTTAATCTCAGTAAGAGGTTTTTTACCAAGGt                                                                                         >  1:348830‑M2/88‑149 (MQ=255)                                                             gatggcgcatgaccttatccttctaagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACTGGAAGCCAGCACGTCTTTAATCTCAGTGAGAGCTTTTTTGCCAAGGt                                                                                         >  2:192093‑M2/88‑149 (MQ=255)                                                             gatggcgcaagaccttatccttctcagtaaaaccttcacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGTCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGt                                                                                         <  1:301610‑M2/62‑1 (MQ=255)                                                              atggcgcatgaccttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACTGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCCAGGtt                                                                                        >  2:480024‑M2/87‑149 (MQ=255)                                                                          cttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                            >  1:994677‑M2/75‑149 (MQ=255)                                                                           ttatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                                           >  1:316713‑M2/74‑149 (MQ=255)                                                                            tatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                               >  1:33370‑M2/73‑112 (MQ=255)                                                                            tatccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                               <  2:33370‑M2/40‑1 (MQ=255)                                                                            tacccttctcagtaagactttaacctgtgatccggttactcattagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGa                                                                          <  2:576621‑M2/77‑1 (MQ=255)                                                                              tccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggaagccagctttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGgcgc                                                                        >  1:420371‑M2/71‑149 (MQ=255)                                                                               ccttctcagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                       >  2:901654‑M2/70‑149 (MQ=255)                                                                                     cagtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCt                                                                 <  1:604359‑M2/86‑1 (MQ=255)                                                                                      agtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGAAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTc                                                                >  1:718423‑M2/63‑149 (MQ=255)                                                                                       gtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGTCAGCACGTCTTTAAtct                                                                                                                 >  1:752334‑M2/62‑99 (MQ=255)                                                                                       gtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGTCAGCACGTCTTTAAtct                                                                                                                 <  2:752334‑M2/38‑1 (MQ=255)                                                                                       gtaaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                               <  1:901654‑M2/88‑1 (MQ=255)                                                                                        taaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                         >  1:829156‑M2/61‑138 (MQ=255)                                                                                        taaaaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                         <  2:829156‑M2/78‑1 (MQ=255)                                                                                           aaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                            >  2:42209‑M2/58‑132 (MQ=255)                                                                                           aaccttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                            <  1:42209‑M2/75‑1 (MQ=255)                                                                                               ttaacctgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                                       >  1:1036598‑M2/54‑149 (MQ=255)                                                                                                     tgtgatccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                                 >  1:380308‑M2/48‑149 (MQ=255)                                                                                                         atccggttactcgtcagcgatgcttgccggtggccagctttccCAGAGACAGTCCACGAAAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCa                                             >  1:334325‑M2/44‑149 (MQ=255)                                                                                                           ccggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTACCATCTTCTCAACCTCGGTACGCTGTACCAGATCAcc                                           <  1:1039524‑M2/108‑1 (MQ=255)                                                                                                             ggttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGa                                         <  1:162393‑M2/110‑1 (MQ=255)                                                                                                              gttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                            <  1:992173‑M2/75‑1 (MQ=255)                                                                                                              gttactcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                            >  2:992173‑M2/39‑113 (MQ=255)                                                                                                                 actcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGAtata                                     <  2:994677‑M2/114‑1 (MQ=255)                                                                                                                 actcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTACACATGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGAtata                                     <  1:1027148‑M2/114‑1 (MQ=255)                                                                                                                   tcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                                   >  2:438208‑M2/34‑149 (MQ=255)                                                                                                                   tcgtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                                   >  1:537986‑M2/34‑149 (MQ=255)                                                                                                                   tcgtcagcgatgcttgccggtggccagttttccCAGAGACAGGCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                                   <  1:382833‑M2/116‑1 (MQ=255)                                                                                                                     gtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTgg                                 >  2:716448‑M2/32‑149 (MQ=255)                                                                                                                     gtcagcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTgg                                 >  2:185259‑M2/32‑149 (MQ=255)                                                                                                                        agcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGGGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTTTACCAGATGACCGATAGAGTGGatg                              >  1:37655‑M2/29‑148 (MQ=255)                                                                                                                        agcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa                              <  1:438208‑M2/121‑1 (MQ=255)                                                                                                                        agcgacgcttgccggttgcaagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATa                              <  2:6707‑M2/121‑1 (MQ=255)                                                                                                                         gcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGCGCTAACCCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAg                             >  1:725487‑M2/28‑149 (MQ=255)                                                                                                                         gcgatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAg                             >  1:936504‑M2/28‑149 (MQ=255)                                                                                                                           gatgcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCt                           <  2:380308‑M2/124‑1 (MQ=255)                                                                                                                              gcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGa                                                <  2:348960‑M2/103‑1 (MQ=255)                                                                                                                              gcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGa                                                >  1:348960‑M2/23‑125 (MQ=255)                                                                                                                              gcttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCt                        >  1:975549‑M2/23‑149 (MQ=255)                                                                                                                               cgtgccggtggccagttttctCACAGACAGTCAACGGGAAGCCAGCATGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                       <  1:889863‑M2/128‑1 (MQ=255)                                                                                                                                ttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATCGCTTCTGc                      >  2:387234‑M2/21‑149 (MQ=255)                                                                                                                                ttgccggtggccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                      >  2:926913‑M2/21‑149 (MQ=255)                                                                                                                                         gccagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa             <  2:537986‑M2/138‑1 (MQ=255)                                                                                                                                           cagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt           >  2:202728‑M2/10‑149 (MQ=255)                                                                                                                                           cagttttccCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt           >  2:599157‑M2/10‑149 (MQ=255)                                                                                                                                                    cAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCACGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTAAGCTGTACCAGCTCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTACCAGAGc  >  2:562141/1‑149 (MQ=255)                                                                                                                                                    cAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGc  >  2:407883/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     ATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGC  >  NC_000913_3_pae_pgi/3439941‑3440236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑