breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 668,742 | C→G | E99Q (GAA→CAA) | rsfS ← | ribosomal silencing factor |
RA | 948,983 | A→T | Y196N (TAT→AAT) | ycaN ← | LysR family putative transcriptional regulator |
RA | 1,275,721 | C→T | E37K (GAA→AAA) | narL ← | response regulator in two‑component regulatory system with NarX |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,513,351 | C→A | L104M (CTG→ATG) | nupC → | nucleoside (except guanosine) transporter |
RA | 3,440,137 | T→G | K298T (AAA→ACA) | rpoA ← | RNA polymerase, alpha subunit |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 4,160,854 | C→T | intergenic (‑64/‑270) | sthA ← / → fabR | pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB |
JC | 4,233,757 | +46 bp | intergenic (‑524/‑583) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
JC | 4,233,840 | +46 bp | intergenic (‑607/‑500) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
RA | 4,294,815 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913_3_KO_pgi | 3423725–3424233 | 3424555–3424239 | 7–831 | 14 [13] | [13] 14 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913_3_KO_pgi | 1207790 = | 7 (0.170) | 27 (0.750) | 25/250 | 0.6 | 76.9% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913_3_KO_pgi | 1209619 = | 10 (0.280) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913_3_KO_pgi | = 1207805 | 7 (0.170) | 23 (0.640) | 19/250 | 1.1 | 74.0% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913_3_KO_pgi | = 1209602 | 10 (0.280) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |