| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_KO_pgi | 4,160,854 | C→T | intergenic (‑64/‑270) | sthA ← / → fabR | pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_KO_pgi | 4,160,854 | 0 | C | T | 92.6% | 71.8 / ‑1.2 | 27 | intergenic (‑64/‑270) | sthA/fabR | pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (10/15); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01 | |||||||||||
GGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACC > NC_000913_3_KO_pgi/4160718‑4160998 | ggCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATcccc > 2:368898/1‑149 (MQ=255) cATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGTTAAGTCAATTGATCCCCGTCa < 2:636966/149‑1 (MQ=255) cAGCGCCTTCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTAt > 1:380480/1‑149 (MQ=255) gcgcCTTCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCt < 1:603198/149‑1 (MQ=255) ttCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTACTCGTACGAGTGTAGACTCGTATGGCTTATCTGTTCTTATACATAAAAGAAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCAGTTATCTGTCtc > 2:83479/1‑148 (MQ=255) aCCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCctttg < 1:154789/149‑1 (MQ=255) gtaGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGcc > 2:607062/1‑149 (MQ=255) aGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCt < 2:579113/149‑1 (MQ=255) gCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTa > 1:703478/1‑149 (MQ=255) gTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGa < 1:620420/149‑1 (MQ=255) gTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGa < 2:423604/149‑1 (MQ=255) ttACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTAt < 2:494611/149‑1 (MQ=255) tACCAGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATc < 1:83479/149‑1 (MQ=255) gTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATATGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATcgacga > 1:419277/1‑149 (MQ=255) aTAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGtt < 2:470412/118‑1 (MQ=255) aTAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGtt > 1:470412/1‑118 (MQ=255) aTAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCGATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGt > 2:663867/1‑149 (MQ=255) aTAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGtt > 2:129356/1‑118 (MQ=255) aTAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGtt < 1:129356/118‑1 (MQ=255) aaGCAACAGAATGGTAACATTTTATCGCGGGTCAGTCAATTCAATCCCGGCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTaa < 1:563077/149‑1 (MQ=255) gCAACAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGc < 1:120567/149‑1 (MQ=255) aCAGAATGGTAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGc < 2:209202/149‑1 (MQ=255) aGAATGATAACATTTTATCGCGGGTCAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGTGCGGCCTTTGCTTTGTTCGTTTCTTACTTTTGCCCTGCCGTTTTATTGGATTTTTATCTACGATACTCTCCGTTTAAGCGGCAg < 1:653850/149‑1 (MQ=255) aaTCGTAACATTTTATCGCGGGTAAGTCAATTCATCCCCGTCATTTATCTGGCCATATCCTGAGCGGCCTTTGCTTTGTTTGTTTCTTCCTTTTGCCCTGACCTTTTATTTGATTTTTTTCGCAGATACTCTCGGTTTACTCGGCCGGt > 2:647311/1‑149 (MQ=255) tAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCg > 1:249475/1‑149 (MQ=255) tAACATTTTATCGCGGGTAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCg < 2:703478/149‑1 (MQ=255) tAAGTCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAAcc < 1:718534/149‑1 (MQ=255) | GGCCCATTGCAGCGCCTTCGCCGCCGGGGCCGGAACCTATTACTATGGCATCGTAATCGTAGGAATGTGGCATGGTAGGGCTTACCTGTTCTTATACATAAAAGCAACAGAATGGTAACATTTTATCGCGGGTAAGCCAATTGATCCCCGTCATTTATCTGGCTATATCCTGAGCGGCCTTTGCTTTGTCTGTTTCTTACTTTTGCCCTGACGTTTTATTGGATTTTTATCGACGATACTCTCCGTTTAAGCGGCAGGTTTCCGCTGTACGTAAAAGAACC > NC_000913_3_KO_pgi/4160718‑4160998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |