| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,840 | +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA | intergenic (‑607/‑500) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233840 | 0 (0.000) | 22 (0.800) +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA |
20/190 | 0.4 | 100% | intergenic (‑607/‑500) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233841 = | NA (NA) | intergenic (‑608/‑499) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
GCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTA > NC_000913_3_KO_pgi/4233707‑4233988 | actacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATg > 1:679830‑M2/135‑149 (MQ=255) cacggtgtaggctggcgctgcttcgaagttcctaaactttcgagagaataggaacttcgaactgcaggtcgacgtatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCACCGTCGATATGTa < 1:92785‑M2/17‑1 (MQ=255) ggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGc > 1:494852‑M2/130‑149 (MQ=255) aggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGAt < 2:114613‑M2/25‑1 (MQ=255) ctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAg < 1:491265‑M2/28‑1 (MQ=255) agctgcttcgaagttccttatctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtttgctttaccgatataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGt < 1:389249‑M2/32‑1 (MQ=255) ttcgaagttcctattctctagaaagtataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc > 2:145160‑M2/112‑149 (MQ=255) atactttctagagactaggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctatcaagcgtggcgcggttaATCATCTTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa > 2:584914‑M2/100‑149 (MQ=255) gaataggaacttccaactgcaggtagacggcgccccggaatgctcgcccaatggtctgagtaaccgctataaagcgcgtcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt < 1:217892‑M2/62‑1 (MQ=255) aattgtaacttcgaactgcaggtcgacggatccccggaatgctccaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGGCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg < 2:301855‑M2/63‑1 (MQ=255) aataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg > 2:498287‑M2/87‑149 (MQ=255) cttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc > 2:491926‑M2/79‑145 (MQ=255) cttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc < 1:491926‑M2/67‑1 (MQ=255) tgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGACGGCTAAGGCGTTCACGACGCTTCCTGCAAAAGATGCCTGATGCgacccggtggtgatt > 2:143361‑M2/71‑142 (MQ=255) gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa > 1:87312‑M2/66‑149 (MQ=255) cgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg > 2:322799‑M2/64‑149 (MQ=255) gacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc > 2:629666‑M2/63‑149 (MQ=255) gcggaatgcttgaccaatgttttgattaactgctataatgcgtggcgcggttaATCATCGTCGATATGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc < 2:527917‑M2/96‑1 (MQ=255) ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc > 1:430130‑M2/54‑149 (MQ=255) ggaaggctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa < 1:646780‑M2/98‑1 (MQ=255) cgaatgctcgaccaacgctcctattaaccgctacaaagcgtggcgcggttaATCATCGTCCATATGTAGGCCGGATAAGGCGTTCACCCCGCATCCGGCAACCGATGCCTGATGCGTCGCGGTCGCGTCTTATCAGGCCTACAGGTCGa < 1:573101‑M2/98‑1 (MQ=255) gaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGAt < 1:629666‑M2/99‑1 (MQ=255) cgcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa > 2:555385‑M2/46‑149 (MQ=255) gcccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCAACCGATGCCTGGTGCGACGCGGTCGCGTCTTAGAAGGCCTACAAGTCGATGCCgatag > 1:112496‑M2/43‑148 (MQ=255) gaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat < 1:555385‑M2/107‑1 (MQ=255) ccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc > 2:89822‑M2/41‑131 (MQ=255) ccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc < 1:89822‑M2/91‑1 (MQ=255) aatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc > 2:432775‑M2/39‑149 (MQ=255) ggttcgcttaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGAAAACGATGACTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTGGATGCCGATATGTACATc < 1:143361‑M2/114‑1 (MQ=255) tctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATcgg > 1:184202‑M2/34‑148 (MQ=255) gattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt > 2:396282‑M2/31‑149 (MQ=255) ttaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc < 2:484507‑M2/101‑1 (MQ=255) ttaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc > 1:484507‑M2/29‑129 (MQ=255) ccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa < 1:18138‑M2/125‑1 (MQ=255) aagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACa > 2:538647‑M2/17‑149 (MQ=255) acgtggcgcgtttaATCATCGTCGATATGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAACCGATGCCTGATGCCACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa < 2:551410‑M2/135‑1 (MQ=255) cgtggcgcggttaATTATCGGCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg < 1:74288‑M2/136‑1 (MQ=255) cgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACga < 1:396282‑M2/141‑1 (MQ=255) taATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgatt > 1:421619‑M2/3‑149 (MQ=255) aATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTa < 1:490915‑M2/148‑1 (MQ=255) | GCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTA > NC_000913_3_KO_pgi/4233707‑4233988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |