| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,840 | +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA | intergenic (‑607/‑500) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233840 | 0 (0.000) | 22 (0.800) +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA |
20/190 | 0.4 | 100% | intergenic (‑607/‑500) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233841 = | NA (NA) | intergenic (‑608/‑499) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
TTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGA > NC_000913_3_KO_pgi/4233696‑4233985 | tagaagagtattgctaacgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTCCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc < 1:272371‑M2/87‑5 (MQ=255) gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCcccggaatgctcgc > 2:217764‑M2/61‑143 (MQ=255) actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatg > 1:679830‑M1/6‑88 (MQ=255) cacggtgtAGGCTGGCGCTGCTTCGAAGTTCCTAAACTTTCGAGAGAATAGGAACTTCGAACTGCAGGTCGACGTATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcaccgtcgatatgta < 1:92785‑M1/146‑64 (MQ=255) ggTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggc > 1:494852‑M1/1‑83 (MQ=255) aGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggat < 2:114613‑M1/149‑72 (MQ=255) cTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataag < 1:491265‑M1/149‑75 (MQ=255) aGCTGCTTCGAAGTTCCTTATCTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtttgctttaccgatataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgt < 1:389249‑M1/149‑79 (MQ=255) ttCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgc > 2:145160‑M1/1‑65 (MQ=255) ataCTTTCTAGAGACTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctatcaagcgtggcgcggttaatcatcttcgatatgtaggccggataaggcgttcacgccgcatccggcaa > 2:584914‑M1/1‑53 (MQ=255) gaATAGGAACTTCCAACTGCAGGTAGACGGCGCCCCGGAATgctcgcccaatggtctgagtaaccgctataaagcgcgtcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgat < 1:217892‑M1/149‑109 (MQ=255) aattgtaacttcgaACTGCAGGTCGACGGATCCCCGGAATgctccaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcggcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatg < 2:301855‑M1/143‑110 (MQ=255) aaTAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatg > 2:498287‑M1/1‑40 (MQ=255) cTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgac < 1:491926‑M1/145‑114 (MQ=255) cTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgac > 2:491926‑M1/1‑32 (MQ=255) tGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggacggctaaggcgttcacgacgcttcctgcaaaagatgcctgatgcgacccggtggtgatt > 2:143361‑M1/1‑24 (MQ=255) gTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatca > 1:87312‑M1/1‑19 (MQ=255) cGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcagg > 2:322799‑M1/1‑17 (MQ=255) gACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggc > 2:629666‑M1/1‑16 (MQ=255) gcgGAATgcttgaccaatgttttgattaactgctataatgcgtggcgcggttaatcatcgtcgatatggaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc < 2:527917‑M1/148‑143 (MQ=255) ccGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtc > 1:430130‑M1/1‑7 (MQ=255) ggAAGgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcacgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcga < 1:646780‑M1/149‑145 (MQ=255) cgaATgctcgaccaacgctcctattaaccgctacaaagcgtggcgcggttaatcatcgtccatatgtaggccggataaggcgttcaccccgcatccggcaaccgatgcctgatgcgtcgcggtcgcgtcttatcaggcctacaggtcga < 1:573101‑M1/148‑145 (MQ=255) gAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggccggataaggcgttcgcgccgcatccggcaaccgatgcctgatgcgacgcggtcgcgtcttatcaggcctacaggtcgat < 1:629666‑M1/149‑146 (MQ=255) | TTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGA > NC_000913_3_KO_pgi/4233696‑4233985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |