New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1395541	9 (0.230)	43 (1.110)	39/280	0.1	82.7%	coding (382/1032 nt)	ynaI	mechanosensitive channel protein, very small conductance
	?	NC_000913_3_KO_pgi	1427777 =	9 (0.230)					coding (950/981 nt)	insH1	IS5 transposase and trans‑activator

GAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/1395398‑1395541 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGCCGCTTGATGATGCGAAACGGGTGCTCCACCCTGGCACGGATGCTG  >  NC_000913_3_KO_pgi/1427777‑1427920                                                                                                                                                                                                                                                                                                  GAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTC                                                                                                                                             <  1:281857/149‑1  AAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCC                                                                                                                                            >  1:552927/1‑149      AGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCC                                                                                                                                        <  1:366714/149‑1        AGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGA                                                                                                                                      <  2:552927/149‑1         GCATAATCCCGGAAAAGAAGGTACTCAGAATATCTTTACCGGCCATAACGACAGCCAGACCACCAATACCAACCAAGGTCAGCAAGCCAGAAAACATCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCAATGGTCACCCCGAc                                                                                                                                     >  2:177970/1‑148                                           TTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAATAAATATTTTCCACCCGCAAAAGGATTTCCAGGGTGAATAACATCGCCAGTTG                                                                                                   >  1:504228/1‑149                                             gagCGGCAATAGCGACACCCAGACGAACAATACCACCAGAGGTAAGCAAGCCAGAAAGGCTCATGCCGAAATGTTTGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGT                                                                                                 <  2:345964/146‑1                                               CCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTT                                                                                                    >  1:334292/1‑144                                               CCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTT                                                                                                    <  2:334292/144‑1                                                  GCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCG                                                                                            >  2:218822/1‑149                                                     ATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTT                                                                                         <  1:585153/149‑1                                                        CCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCCACACTTATCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTC                                                                                      >  1:161859/1‑149                                                                CAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCC                                                                              >  1:84617/1‑149                                                                     CACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCCGCCACTTGT                                                                         >  1:219549/1‑149                                                                     CACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCA                                                                                  <  2:502135/140‑1                                                                     CACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCA                                                                                  >  1:502135/1‑140                                                                         AATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCT                                                                     <  2:84617/149‑1                                                                         AATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCT                                                                     >  1:413538/1‑149                                                                            ACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGC                                                                  <  1:9516/149‑1                                                                              CACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTT                                                                >  2:243991/1‑149                                                                              CACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAA                                                                                                               <  2:533741/102‑1                                                                              CACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAA                                                                                                               >  1:533741/1‑102                                                                                  AAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCAC                                                            >  1:458448/1‑149                                                                                   AAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCA                                                             <  1:540145/147‑1                                                                                   AAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCA                                                             >  2:540145/1‑147                                                                                     aGTCAGCAAGCAAGAAGGGGTAGTGCACATATGTTGTCCATAAAGCAGAACAAGCACCACTGGTCCAGCCGAAACAGGTTGGCCAGGGTGAATTACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAA                                                         <  2:32847/148‑1                                                                                       TCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCA                                                                                                       >  1:554524/1‑101                                                                                       TCAGCAAGCCAGAAAGCCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCA                                                                                                       <  2:554524/101‑1                                                                                            AAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAAC                                                  <  1:243991/149‑1                                                                                             AGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTT                                                                           >  1:178032/1‑123                                                                                             AGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTT                                                                           <  2:178032/123‑1                                                                                               CCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTG                                                                                                                             >  2:307733/1‑71                                                                                               CCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTG                                                                                                                             <  1:307733/71‑1                                                                                               CCAGAAAGGCTCACGACGAAATGTGCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGC                                               <  1:503104/149‑1                                                                                                    AAGGCTCAGGCCGAAATGTACGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTCGCCCGGGTGAATAACTTCGCCAGGTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGCCGCTT                                          <  1:313283/149‑1                                                                                                           ATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGTCAGGGTGAATAACATCGCCAGTTGGTTATC                                                                                             >  2:377361/1‑91                                                                                                           ATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGTCAGGGTGAATAACATCGCCAGTTGGTTATC                                                                                             <  1:377361/91‑1                                                                                                           ATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGCCGCTTGCTGATc                                   <  2:529206/149‑2                                                                                                               CGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCG                                                                                            >  2:171961/1‑88                                                                                                               CGAAATGTTCGCCATAAAGCAGAACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCG                                                                                            <  1:171961/88‑1                                                                                                                                     AACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGCCGCTTGATGATGCGAAACGGGTGCTCCACCCTGGCACG         >  2:460333/1‑149                                                                                                                                      ACAAGCACCACTGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGCCGCTTGATGATGCGAAACGGGTGCTCCACCCTGGCACGG        >  1:495602/1‑149                                                                                                                                            cCCACTCGTCCACCCTAAAGAGTTTGGCCAGTGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTGCACGAAGCCGAACTGCCCGACGATTATCCGACACGAGTGCTCCACCCTGGCACGGATGCTc  <  1:652257/148‑2                                                                                                                                                                                                                                                                                                  GAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCACCAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/1395398‑1395541 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGTCCACCCGAAACAGGTTGGCCAGGGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAGCCCCTTGTATCTGGCTTTCACGAAGCCGAACTGCCGCTTGATGATGCGAAACGGGTGCTCCACCCTGGCACGGATGCTG  >  NC_000913_3_KO_pgi/1427777‑1427920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.