BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	1978503–1979272	1979278	7–776	193 [0]	[0] 193	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1978502	0 (0.000)	178 (0.960)	95/180	0.3	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
*	?	NC_000913_3_KO_pgi	1979279 =	0 (0.000)	178 (0.960)	95/180	0.3	100%	intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

Only 100 of 193 total aligned reads displayed.

CAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATG  >  NC_000913_3_KO_pgi/1979184‑1979377

ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtata                                                                                                 <  1:3921301‑M2/4‑1 (MQ=255)

ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  <  2:2264774‑M2/3‑1 (MQ=255)

ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  >  2:1734763‑M2/96‑98 (MQ=255)

ctcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  >  1:4286559‑M2/96‑98 (MQ=255)

ctcgtcactacacgcacatacaacggaggggggctacgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  >  1:1778461‑M2/96‑98 (MQ=255)

 tcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  >  2:2055232‑M2/95‑97 (MQ=255)

 tcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                  >  2:518575‑M2/95‑97 (MQ=255)

   gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                               <  1:4008004‑M2/6‑1 (MQ=255)

   gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGt                                                                                              >  2:4046386‑M2/93‑99 (MQ=255)

   ggcactacgcgcacatacaacgggggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAg                                                                                               <  1:2732881‑M2/6‑1 (MQ=255)

     cactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCa                                                                                            >  1:2426091‑M2/91‑99 (MQ=255)

       ctacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATa                                                                                          <  2:3013847‑M2/11‑1 (MQ=255)

         acacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACtt                                                                                       >  1:774655‑M2/87‑100 (MQ=255)

            cgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACtttt                                                                                     <  2:2273697‑M2/16‑1 (MQ=255)

             gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGCCATACttttgt                                                                                   <  1:2474480‑M2/18‑1 (MQ=255)

              cacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgt                                                                                   <  2:1348130‑M2/18‑1 (MQ=255)

                catacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgttt                                                                                 <  2:3714904‑M2/20‑1 (MQ=255)

                 atacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACttttgtttt                                                                                <  2:3869948‑M2/21‑1 (MQ=255)

                  tacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                              <  1:2253711‑M2/23‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacatagatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:3793273‑M2/24‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:731317‑M2/24‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:4830830‑M2/24‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             <  1:754141‑M2/24‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                              <  2:1441089‑M2/23‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                              <  2:385628‑M2/23‑1 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                            >  1:4540822‑M2/76‑100 (MQ=255)

                    caacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                            >  1:4477351‑M2/76‑100 (MQ=255)

                     aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                             >  2:1181532‑M2/75‑98 (MQ=255)

                     aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgtg                                                                           <  1:3728424‑M2/26‑1 (MQ=255)

                     aacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                            >  2:766273‑M2/75‑99 (MQ=255)

                      acggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgtg                                                                           >  2:1887983‑M2/74‑99 (MQ=255)

                       cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTa                                                                         >  1:1629844‑M2/73‑100 (MQ=255)

                         gaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAtt                                                                       <  1:3248110‑M2/30‑1 (MQ=255)

                            gggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                            <  2:2577566‑M2/25‑1 (MQ=255)

                               ggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAatn                                                                 <  1:4109669‑M2/36‑2 (MQ=255)

                               ggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                  <  2:113811‑M2/35‑1 (MQ=255)

                               ggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                                                 <  1:2019365‑M2/36‑1 (MQ=255)

                                gctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCt                                                                <  1:544626‑M2/37‑1 (MQ=255)

                                 ctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTg                                                               <  1:2198109‑M2/38‑1 (MQ=255)

                                  tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTg                                                               >  2:4462959‑M2/62‑99 (MQ=255)

                                    cgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                              <  2:2955355‑M2/39‑1 (MQ=255)

                                     gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGtt                                                             >  2:4832652‑M2/59‑98 (MQ=255)

                                     gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            >  1:3300‑M2/59‑99 (MQ=255)

                                     gattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa                                                            <  2:816869‑M2/41‑1 (MQ=255)

                                       ttttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaa                                                          >  1:1341128‑M2/57‑99 (MQ=255)

                                        tttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaa                                                          >  1:433878‑M2/56‑98 (MQ=255)

                                         ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaa                                                         <  2:2133653‑M2/44‑1 (MQ=255)

                                         ttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAg                                                       >  1:3071776‑M2/55‑100 (MQ=255)

                                            aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttt                                                    >  1:1222217‑M2/52‑100 (MQ=255)

                                             ataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttt                                                    >  2:2631492‑M2/51‑99 (MQ=255)

                                               aatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                 >  1:330636‑M2/49‑100 (MQ=255)

                                                atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                  <  2:920178‑M2/51‑1 (MQ=255)

                                                atgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                                                 <  1:1624182‑M2/52‑1 (MQ=255)

                                                 tgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttttt                                                  >  1:2662278‑M2/47‑97 (MQ=255)

                                                 tgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgc                                               <  1:4470048‑M2/54‑1 (MQ=255)

                                                   cgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgcta                                             >  1:3701002‑M2/45‑100 (MQ=255)

                                                   cgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTAcgcta                                             >  1:4182777‑M2/45‑100 (MQ=255)

                                                       atgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacg                                           <  2:3414429‑M2/58‑1 (MQ=255)

                                                        tgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                          >  1:1951599‑M2/40‑98 (MQ=255)

                                                        tgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTAAATCTGTTAAAAAGTTTTTcgc                                               >  2:4292014‑M2/40‑93 (MQ=255)

                                                         gcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                          >  2:1124603‑M2/39‑97 (MQ=255)

                                                          cagatcacactaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgcta                                        <  2:3217799‑M2/61‑1 (MQ=255)

                                                           agatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagca                                     <  1:2238610‑M2/64‑1 (MQ=255)

                                                           agatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTag                                       >  2:1207058‑M2/37‑98 (MQ=255)

                                                           agatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTCGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                      >  1:4948154‑M2/37‑99 (MQ=255)

                                                            gatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                      >  1:1099692‑M2/36‑98 (MQ=255)

                                                            gatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                      >  2:4229087‑M2/36‑98 (MQ=255)

                                                            gatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagc                                      >  1:2973504‑M2/36‑98 (MQ=255)

                                                             atcacacaaaatactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                    <  2:3331818‑M2/65‑1 (MQ=255)

                                                             atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                   >  1:563160‑M2/35‑100 (MQ=255)

                                                             atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                   <  1:3451260‑M2/66‑1 (MQ=255)

                                                             atcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTag                                       >  2:4680384‑M2/35‑96 (MQ=255)

                                                              tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgc                                               >  2:3484585‑M2/34‑87 (MQ=255)

                                                              tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                   <  2:4073630‑M2/66‑1 (MQ=255)

                                                              tcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaag                                   >  2:192872‑M2/34‑99 (MQ=255)

                                                                 cacaaaacactcgattacttaacataaatgTATAAGTCATACTCTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagca                                 >  2:2109114‑M2/31‑98 (MQ=255)

                                                                 cacaaaacactcaattacttaacataactgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagca                                 >  2:4129879‑M2/31‑98 (MQ=255)

                                                                 cacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTTTTTACGCTagcaagca                                 >  2:3565957‑M2/31‑98 (MQ=255)

                                                                 cacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                >  1:597482‑M2/31‑99 (MQ=255)

                                                                  acaaaacacccaattacttaacataaatgTATAAGTCATACTTTTTTTTTGAGTGTATTTCAATCTGTTAAAACGTTTTTCGCTACGCTAgcaagcaa                                >  2:661885‑M2/30‑98 (MQ=255)

                                                                   caaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                             <  1:3358657‑M2/72‑1 (MQ=255)

                                                                     aaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa                             <  2:4242603‑M2/72‑1 (MQ=255)

                                                                     aaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                            <  1:2094423‑M2/73‑1 (MQ=255)

                                                                         actcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                        >  1:1340464‑M2/23‑99 (MQ=255)

                                                                          ctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                        >  1:712541‑M2/22‑98 (MQ=255)

                                                                            caattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                     >  1:171438‑M2/20‑99 (MQ=255)

                                                                            caattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                     >  1:1735619‑M2/20‑99 (MQ=255)

                                                                               ttacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTTGCTACGCTAGCAAGCAAAAATGAAACCGGaa                  >  2:2559252‑M2/17‑99 (MQ=255)

                                                                                  cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat              >  1:3161056‑M2/14‑100 (MQ=255)

                                                                                  cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataat              >  1:3066249‑M2/14‑100 (MQ=255)

                                                                                  cttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaataa               <  2:1614297‑M2/86‑1 (MQ=255)

                                                                                   ttaacataaatgTATACGTCATACTTTTGCTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATc             >  1:1571253‑M2/13‑100 (MQ=255)

                                                                                       cataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGaaa         >  1:2070875‑M2/9‑100 (MQ=255)

                                                                                       cataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGa           >  1:3630340‑M2/9‑98 (MQ=255)

                                                                                            atgTATAAGTCATACTTTTTTTTTTTGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATgg      >  2:1593171‑M2/4‑98 (MQ=255)

                                                                                            atgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATggg     <  2:4477741‑M2/96‑1 (MQ=255)

                                                                                             tgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt   >  1:1972896‑M2/3‑100 (MQ=255)

                                                                                              gTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGAt   >  2:3624490‑M2/2‑99 (MQ=255)

                                                                                              gTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg  >  1:790200‑M2/2‑100 (MQ=255)

                                                                                              gTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg  >  1:1516152‑M2/2‑100 (MQ=255)

CAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATG  >  NC_000913_3_KO_pgi/1979184‑1979377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`