BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	1978503–1979272	1979278	7–776	193 [0]	[0] 193	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1978502	0 (0.000)	178 (0.960)	95/180	0.3	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
*	?	NC_000913_3_KO_pgi	1979279 =	0 (0.000)	178 (0.960)	95/180	0.3	100%	intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

Only 100 of 193 total aligned reads displayed.

TACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCC  >  NC_000913_3_KO_pgi/1978406‑1978600

tACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATG                                                                                                    <  1:1303782‑M1/97‑1 (MQ=255)

 aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                 >  1:4027240‑M1/1‑96 (MQ=255)

  cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                 <  2:2264774‑M1/98‑4 (MQ=255)

   tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                 >  2:518575‑M1/1‑94 (MQ=255)

     gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                             >  2:4046386‑M1/1‑92 (MQ=255)

     gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                             >  2:2117767‑M1/1‑92 (MQ=255)

     gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagt                                                                                             >  1:3502303‑M1/1‑92 (MQ=255)

         cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcata                                                                                         <  2:3013847‑M1/99‑12 (MQ=255)

           acacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactt                                                                                      >  1:1529336‑M1/1‑86 (MQ=255)

            cacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatact                                                                                       <  1:2688273‑M1/98‑14 (MQ=255)

            cacGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatact                                                                                       <  1:1738517‑M1/98‑14 (MQ=255)

              cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                   <  1:4380200‑M1/100‑18 (MQ=255)

              cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                   >  1:2552295‑M1/1‑83 (MQ=255)

              cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttg                                                                                   >  1:4327937‑M1/1‑83 (MQ=255)

              cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttt                                                                                    <  2:2273697‑M1/99‑17 (MQ=255)

               gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagccatacttttgt                                                                                  <  1:2471811‑M1/100‑19 (MQ=255)

                cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                 >  1:4248991‑M1/1‑81 (MQ=255)

                  caTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt                                                                                <  2:1430249‑M1/99‑21 (MQ=255)

                  caTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt                                                                                <  2:3714904‑M1/99‑21 (MQ=255)

                   aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtttt                                                                               <  2:3869948‑M1/99‑22 (MQ=255)

                     aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACGAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                            >  1:643455‑M1/1‑76 (MQ=255)

                     aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                            >  1:780054‑M1/1‑76 (MQ=255)

                      cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                           >  1:592774‑M1/1‑75 (MQ=255)

                      cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                            <  1:307449‑M1/99‑25 (MQ=255)

                      cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                             <  2:385628‑M1/98‑24 (MQ=255)

                      cAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                             <  2:2079020‑M1/98‑24 (MQ=255)

                       aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                          >  1:3344805‑M1/1‑74 (MQ=255)

                       aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                          <  1:3728536‑M1/100‑27 (MQ=255)

                       aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                           >  2:766273‑M1/1‑74 (MQ=255)

                       aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggg                                                                            >  2:1181532‑M1/1‑74 (MQ=255)

                        aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                          >  2:1133995‑M1/1‑73 (MQ=255)

                         cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                        >  1:1629811‑M1/1‑72 (MQ=255)

                         cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                        >  1:1631376‑M1/1‑72 (MQ=255)

                         cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtg                                                                          >  2:1726201‑M1/1‑72 (MQ=255)

                           gAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatt                                                                      >  1:414251‑M1/1‑70 (MQ=255)

                            aGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatt                                                                      <  2:1513541‑M1/99‑31 (MQ=255)

                              gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggt                                                                           <  2:2577566‑M1/92‑26 (MQ=255)

                                 ggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatc                                                                <  1:2020287‑M1/100‑37 (MQ=255)

                                 ggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                 <  2:114552‑M1/99‑36 (MQ=255)

                                   cTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctg                                                              <  1:2989060‑M1/100‑39 (MQ=255)

                                    tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                             >  1:1863509‑M1/1‑61 (MQ=255)

                                    tGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctg                                                              >  2:4462959‑M1/1‑61 (MQ=255)

                                      cGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                             <  1:56031‑M1/98‑40 (MQ=255)

                                      cGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                             <  2:2955355‑M1/98‑40 (MQ=255)

                                       gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtta                                                           >  1:4509319‑M1/1‑58 (MQ=255)

                                       gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtta                                                           >  1:3300‑M1/1‑58 (MQ=255)

                                       gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtta                                                           <  2:816869‑M1/99‑42 (MQ=255)

                                       gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                            >  2:4832652‑M1/1‑58 (MQ=255)

                                         ttttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                         >  1:1340565‑M1/1‑56 (MQ=255)

                                          tttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                         >  1:3295229‑M1/1‑55 (MQ=255)

                                           ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaag                                                      >  1:2017108‑M1/1‑54 (MQ=255)

                                           ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                       <  1:2240851‑M1/99‑46 (MQ=255)

                                            tCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagt                                                     >  1:4859781‑M1/1‑53 (MQ=255)

                                            tCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaag                                                      <  1:2299546‑M1/99‑47 (MQ=255)

                                              aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                   >  1:1222217‑M1/1‑51 (MQ=255)

                                               ataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttt                                                   >  2:2631492‑M1/1‑50 (MQ=255)

                                                  atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttc                                                <  1:519975‑M1/99‑53 (MQ=255)

                                                  atGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                 <  2:920178‑M1/98‑52 (MQ=255)

                                                   tGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgc                                              <  1:4470048‑M1/100‑55 (MQ=255)

                                                     cGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgct                                             <  2:2046204‑M1/99‑56 (MQ=255)

                                                     cGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaa                                                       <  1:1594956‑M1/89‑46 (MQ=255)

                                                      gTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctac                                           >  1:2186783‑M1/1‑43 (MQ=255)

                                                         aTGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacg                                          <  2:3414429‑M1/98‑59 (MQ=255)

                                                          tGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                         >  1:760087‑M1/1‑39 (MQ=255)

                                                          tGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttaaatctgttaaaaagtttttcgc                                              >  2:4292014‑M1/1‑39 (MQ=255)

                                                            cAGATCACACTAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcta                                       <  2:3217799‑M1/98‑62 (MQ=255)

                                                              gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagc                                     >  1:1099692‑M1/1‑35 (MQ=255)

                                                              gATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagc                                     >  2:4229087‑M1/1‑35 (MQ=255)

                                                               aTCACACAAAATACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaa                                   <  2:3331818‑M1/99‑66 (MQ=255)

                                                               aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctag                                      >  2:4680384‑M1/1‑34 (MQ=255)

                                                                tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                  <  2:4073630‑M1/99‑67 (MQ=255)

                                                                tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaag                                  >  1:4687624‑M1/1‑33 (MQ=255)

                                                                tCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgc                                              >  2:3484585‑M1/1‑33 (MQ=255)

                                                                   cacaAAACACTCAATTACTTAACATAACTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagca                                >  2:4129879‑M1/1‑30 (MQ=255)

                                                                   cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttttttacgctagcaagca                                >  2:3565957‑M1/1‑30 (MQ=255)

                                                                    acaAAACACCCAATTACTTAACATAAATGtataagtcatactttttttttgagtgtatttcaatctgttaaaacgtttttcgctacgctagcaagcaa                               >  2:661885‑M1/1‑29 (MQ=255)

                                                                     caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                            >  1:2233123‑M1/1‑28 (MQ=255)

                                                                     caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                            <  1:3360315‑M1/100‑73 (MQ=255)

                                                                     caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                            >  1:3719507‑M1/1‑28 (MQ=255)

                                                                       aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatg                          <  1:394936‑M1/100‑75 (MQ=255)

                                                                       aaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaa                            <  2:4242603‑M1/98‑73 (MQ=255)

                                                                         acacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                         <  2:1077653‑M1/99‑76 (MQ=255)

                                                                          cacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                       <  1:172937‑M1/100‑78 (MQ=255)

                                                                           acTCAATTACTTAACATAAATGtataagtcatactgtggttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaac                      <  1:2817593‑M1/100‑79 (MQ=255)

                                                                            cTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaca                     <  1:3067332‑M1/100‑80 (MQ=255)

                                                                            cTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                       >  1:713572‑M1/1‑21 (MQ=255)

                                                                              cAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                    >  1:1373727‑M1/1‑19 (MQ=255)

                                                                               aaTTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagg                   <  1:3644977‑M1/99‑82 (MQ=255)

                                                                                 ttACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttttgctacgctagcaagcaaaaatgaaaccggaa                 >  2:2559252‑M1/1‑16 (MQ=255)

                                                                                    cTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa              <  2:1614261‑M1/99‑87 (MQ=255)

                                                                                     ttAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc            >  1:3191203‑M1/1‑12 (MQ=255)

                                                                                      tAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc            >  1:1953854‑M1/1‑11 (MQ=255)

                                                                                       aaCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatc            >  2:1897256‑M1/1‑10 (MQ=255)

                                                                                         cATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa        >  1:2070875‑M1/1‑8 (MQ=255)

                                                                                          aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat       <  1:739175‑M1/100‑94 (MQ=255)

                                                                                          aTAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaa        >  2:1337817‑M1/1‑7 (MQ=255)

                                                                                           tAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatg      <  1:3879982‑M1/100‑95 (MQ=255)

                                                                                           tAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaat       >  2:179552‑M1/1‑6 (MQ=255)

                                                                                              aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggg    <  2:4477741‑M1/99‑97 (MQ=255)

                                                                                                gtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat  >  2:3624490‑M1/1‑1 (MQ=255)

TACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCC  >  NC_000913_3_KO_pgi/1978406‑1978600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`