New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 624576 =198 (0.980)21 (0.160)
+TGTCTGAGCACTTTTGTACTTTGTCATCTGACTAA
10/126 14.4 13.6% intergenic (‑66/‑309) fepB/entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
?NC_000913_3_KO_pgi = 3046145 221 (1.100)intergenic (‑244/+23) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 198 total aligned reads displayed.

GAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAG  >  NC_000913_3_KO_pgi/624477‑624672
                                                                                                   |                                                                                                
gAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCg                                                                                                  >  1:3062823/1‑100 (MQ=255)
  aGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGt                                                                                                 <  2:4370678/99‑1 (MQ=255)
  aGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTc                                                                                                <  1:2892699/100‑1 (MQ=255)
   gggCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTc                                                                                                >  2:590782/1‑99 (MQ=255)
    ggCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTcc                                                                                               <  2:74198/99‑1 (MQ=255)
    ggCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTcc                                                                                               <  2:2048328/99‑1 (MQ=255)
       gTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTc                                                                                           <  1:3281790/100‑1 (MQ=255)
         tGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGc                                                                                         >  1:3460065/1‑100 (MQ=255)
          gCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGcc                                                                                        >  1:154878/1‑100 (MQ=255)
           cGGTAGAGCGGGGCGAGTCTCACTAATCAGCTTCCTGTTATTAATAAAGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGc                                                                                         <  2:4619300/98‑1 (MQ=255)
           cGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGc                                                                                         <  2:3143646/98‑1 (MQ=255)
             gTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATATGGTTAAGGGGGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGcccc                                                                                      <  2:4809340/99‑1 (MQ=255)
               agagCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGCCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCtt                                                                                    >  2:1909737/1‑99 (MQ=255)
                gagCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTg                                                                                  >  1:2440677/1‑100 (MQ=255)
                gagCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTg                                                                                  <  1:4430257/100‑1 (MQ=255)
                 agCGGGGCGAGTCTCACAAATCAGCGTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTg                                                                                  <  2:3325090/99‑1 (MQ=255)
                   cGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGccc                                                                                       <  2:3517874/92‑1 (MQ=255)
                   cGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTgggg                                                                               <  1:4223814/100‑1 (MQ=255)
                   cGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTgggg                                                                               <  1:1040160/100‑1 (MQ=255)
                   cGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTgggg                                                                               <  1:681645/100‑1 (MQ=255)
                     gggCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTggg                                                                                >  2:456303/1‑97 (MQ=255)
                        cGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAgg                                                                          >  1:4287455/1‑100 (MQ=255)
                         gAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAggg                                                                         >  1:4685622/1‑100 (MQ=255)
                         gAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAggg                                                                         <  1:4062763/100‑1 (MQ=255)
                          aGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAggg                                                                         >  2:1757973/1‑99 (MQ=255)
                          aGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAggg                                                                         <  2:3460112/99‑1 (MQ=255)
                           gTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                        >  1:1402944/1‑99 (MQ=255)
                            tctcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                        >  2:990867/1‑98 (MQ=255)
                            tctcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGt                                                                        >  2:1439913/1‑98 (MQ=255)
                             ctcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAg                                                                     >  1:1869661/1‑100 (MQ=255)
                             ctcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAg                                                                     >  1:3193654/1‑100 (MQ=255)
                              tcACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                    >  1:3954751/1‑100 (MQ=255)
                               cacaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAggg                                                                   >  1:2737982/1‑100 (MQ=255)
                               cacaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                    >  2:2753156/1‑99 (MQ=255)
                                acaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAgg                                                                    <  2:401861/98‑1 (MQ=255)
                                acaAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAg                                                                     <  1:4032726/97‑1 (MQ=255)
                                 caAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGt                                                                  >  1:3264608/1‑99 (MQ=255)
                                   aaTCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                               <  1:268374/100‑1 (MQ=255)
                                   aaTCAGCTTCCTGTTACTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                               >  1:3172796/1‑100 (MQ=255)
                                       aGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAggg                                                             >  2:1325388/1‑98 (MQ=255)
                                       aGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAggg                                                             >  2:1694136/1‑98 (MQ=255)
                                       aGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGa                                                           <  1:294700/100‑1 (MQ=255)
                                       aGCGTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATGCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAg                                                               <  2:3792150/96‑1 (MQ=255)
                                        gCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGaa                                                          >  1:3701128/1‑100 (MQ=255)
                                            ccTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcag                                                       >  2:2432486/1‑99 (MQ=255)
                                            ccTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcag                                                       >  2:1364741/1‑99 (MQ=255)
                                            ccTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAca                                                        <  2:2839063/98‑1 (MQ=255)
                                            ccTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAca                                                        <  1:1784226/98‑1 (MQ=255)
                                             cTGTTATTACTAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTTCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagcc                                                     <  1:4971275/100‑1 (MQ=255)
                                             cTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcag                                                       <  2:4210415/98‑1 (MQ=255)
                                               gTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagccag                                                   >  1:1801983/1‑100 (MQ=255)
                                               gTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagccag                                                   >  1:3239002/1‑100 (MQ=255)
                                                ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGGTAGGGGGAGGGGAAcagccag                                                   >  2:2691610/1‑99 (MQ=255)
                                                ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCACGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAAcagccag                                                   <  1:4878431/99‑1 (MQ=255)
                                                   ttaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACt                                               >  1:743641/1‑100 (MQ=255)
                                                     aataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTgg                                             >  1:1027008/1‑100 (MQ=255)
                                                     aataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTgg                                             >  1:862738/1‑100 (MQ=255)
                                                          ggTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCg                                         >  1:2663529/1‑99 (MQ=255)
                                                           gTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGaa                                       >  1:1865286/1‑100 (MQ=255)
                                                           gTTAAGGGCGAAAGGACAGCTGCGAAAAAGCGCGAAATCCTCCCCCTCCCCCCTTGGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGa                                        <  2:4684230/99‑1 (MQ=18)
                                                                gggCGAAAGGACAAAGTGGAAAAGGCGCAAATCCCGGCCCCTCCCCCTTTGGGGGAGAGGGTTAGGGGGAGGGGAACAGCCAGCACTGGTGCGAACAt                                    <  2:2166808/98‑1 (MQ=16)
                                                                 ggCGTAATGACAAATTCGAAAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGGGGGTTAGGGTGAGGGGACCAGCCAGCACTGGTGCGAACATTa                                  <  1:2543316/99‑1 (MQ=255)
                                                                 ggCGAAATGACAAATTCGACAAGGCGCACAAGCCGTCCCCTCGCCCCTGGGGGGGGGGGGTTAGGGGGGGGGGAACAGCCAGCACTGGTGCGAAc                                      <  2:2434982/95‑1 (MQ=255)
                                                                  gCGTAATGCCAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTGGGGGGGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTaa                                 <  2:1743104/99‑1 (MQ=255)
                                                                  gCGTAATGACAAATTCGACAAAGCGCACAAGCCGTCCCCTCGCCCCTTTGGGGGGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTaa                                 <  2:800158/99‑1 (MQ=255)
                                                                   cgaaaTGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGGGAGGGTTAGGGGGGGGGGAACAGCCAGCACTGGTGCGAACATTAAc                                <  2:607564/96‑1 (MQ=37)
                                                                     tAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCt                             >  1:1933906/1‑100 (MQ=255)
                                                                      aaTGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCt                             >  1:2358300/1‑99 (MQ=255)
                                                                          aCAAATTCGACAAAGCGCACAATCCGTCCCCCCGCCCCTTTGGGGAGAGGGCTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTCACCCTCAcc                         >  2:3158214/1‑99 (MQ=255)
                                                                           cAATTTCGAAAAAGCGCACAATCCGTCCCCTCGCCCCTTGGGGGAGGGGGTTAGGGGGGGGGGACCAGCCAGCACTGGTGCGAACATTAACCGTCAccc                        <  2:4934965/99‑1 (MQ=25)
                                                                           cAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGCTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAccc                        >  1:3768089/1‑99 (MQ=255)
                                                                            aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAccc                        <  1:4892763/98‑1 (MQ=255)
                                                                            aaaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAccc                        >  2:1672983/1‑98 (MQ=255)
                                                                            aaaTTCGACAAAGCGCACAATCCGTCCCCCCGCCCCTTTGGGGAGAGGGTTAGGGTGGGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCAc                          <  2:4123551/96‑1 (MQ=255)
                                                                             aaTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGGGGGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCAcc                         <  1:4760677/96‑1 (MQ=255)
                                                                             aaTTCAACAAGGCGCACAACCCGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAg                     <  1:3617253/100‑1 (MQ=21)
                                                                              aTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAg                     >  2:2556341/1‑99 (MQ=255)
                                                                              aTTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGc                    <  1:1292590/100‑1 (MQ=255)
                                                                                 cGACAAAGCGCACAATCCGTCCCCCCGCCCCTTTGGGGAGAGGGTTAGGGGGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGccc                  <  1:3668266/99‑1 (MQ=255)
                                                                                   aCAAAGCGCACAATGCGGCCCCGCGCCCCTGGGGGGGGGGGGTGGGGGTGGGGGGAACAGCCAGCa                                                 <  2:1067280/66‑1 (MQ=255)
                                                                                     aaaGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAc              >  2:3937330/1‑99 (MQ=255)
                                                                                      aaGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAccc            >  1:1501865/1‑100 (MQ=255)
                                                                                      aaGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGGGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAcc             <  1:3336349/99‑1 (MQ=255)
                                                                                      aaGCGCACAATCCGTCCCCCCGCCCCTTTGGGGGGAGGGTTAGGGTGGGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCAccc            <  1:2392448/100‑1 (MQ=255)
                                                                                      aaGCCCACAACCCGCCCCCTCGCCCCTTTGGGGAGGGGGTAAGGGGGAGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCa               <  1:991685/97‑1 (MQ=21)
                                                                                       aGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCt           >  1:480182/1‑100 (MQ=255)
                                                                                       aGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCt           >  1:477874/1‑100 (MQ=255)
                                                                                         cgcACAATCCGTCCCCTCGCCCCTTTCGGGAGAGGGTTACGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTg          >  1:4595586/1‑99 (MQ=255)
                                                                                          gcACAATCCGTCCCCTCGCCCCTTTGGGGGGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGa        <  1:1436224/100‑1 (MQ=255)
                                                                                          gcACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTgg         >  1:4357038/1‑99 (MQ=255)
                                                                                          gcACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTgg         >  1:4670224/1‑99 (MQ=255)
                                                                                          gcACAATCCGTCCCCTCGCCCCTTGGGGGAGGGGGTTAGGGTGGGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGa        <  1:14451/100‑1 (MQ=255)
                                                                                          gcACAAGCCGCCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCt           <  2:2731330/97‑1 (MQ=255)
                                                                                           cacaATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTgg         >  1:3654871/1‑98 (MQ=255)
                                                                                            ccaATCCGCCCCCTCGCCCCTTTGGGGAGGGGGTTAGGGGGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAg      <  1:3663753/99‑1 (MQ=21)
                                                                                            acaATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTgg         >  2:3404743/1‑97 (MQ=255)
                                                                                             caATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAgg     >  1:541784/1‑100 (MQ=255)
                                                                                                tCCGTCCCCTCGCCCCTTTGGGGGGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGgag  <  1:2093743/100‑1 (MQ=255)
                                                                                                  cGTCCCCTCGCCCTTTTGGGGGGAGGGTTAGGTTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACTCCAGCCCTCACCCTGGAAGGga   <  2:369493/97‑1 (MQ=255)
                                                                                                  cGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGga   >  2:2306306/1‑97 (MQ=255)
                                                                                                   |                                                                                                
GAAGGGCGTTGCGGTAGAGCGGGGCGAGTCTCACAAATCAGCTTCCTGTTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAG  >  NC_000913_3_KO_pgi/624477‑624672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.