New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 624576 =198 (0.980)21 (0.160)
+TGTCTGAGCACTTTTGTACTTTGTCATCTGACTAA
10/126 14.4 13.6% intergenic (‑66/‑309) fepB/entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
?NC_000913_3_KO_pgi = 3046145 221 (1.100)intergenic (‑244/+23) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_KO_pgi/624620‑624576
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAAGGCGCTTCGGCGCCTTTTTAGTCAGATGACAAAGTACAAAAG  <  NC_000913_3_KO_pgi/3046145‑3046101
                                             |||||||||||||||||||||||||||||||||||                                             
cCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTT                           <  1:1063070/99‑1
 CTGTTCCCCTCCCCCTAACCCGCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTT                          <  1:1282886/100‑1
 CTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTT                          <  1:84098/100‑1
    TTCCGCTAACCCTAACCCCCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTA                        <  2:1277691/99‑1
    TTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAG                       <  1:2991002/100‑1
    TTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTA                        <  2:3098417/99‑1
    TTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTA                        <  2:2115734/99‑1
    TTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTT                         <  1:1640884/98‑1
    TTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTT                          <  2:3395140/97‑1
    TTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTT                          <  2:1885502/97‑1
       CCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTCA                    <  1:2903873/100‑1
       CCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTC                     <  1:2996915/99‑1
       CCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTC                     <  1:601078/99‑1
       CCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTC                     <  1:2494033/99‑1
       CCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTC                     <  1:2296109/99‑1
       CCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTC                     <  2:2619155/99‑1
        CCTCACCCTAATCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTCA                    <  2:3329982/99‑1
        CCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTCAG                   <  1:1071847/100‑1
            ACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTCAGATG                <  1:1636269/99‑1
            ACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTCAGATG                <  1:1572778/99‑1
                          CCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCTTTTTAGTCAGATGACAAAGTACAAAAG  <  1:2804155/99‑1
                                             |||||||||||||||||||||||||||||||||||                                             
GCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_KO_pgi/624620‑624576
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AAAGGCGCTTCGGCGCCTTTTTAGTCAGATGACAAAGTACAAAAG  <  NC_000913_3_KO_pgi/3046145‑3046101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.